Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of Fusarium oxysporum Isolated from Paprika in Korea

  • Published : 2007.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In the present study we first report in Korea the identification and characterization of Fusarium oxysporum isolated from rotten stems and roots of paprika (Capsicum annuum var. grossum) at Masan, Kyungsangnamdo in 2006. The fungal species produced white aerial mycelia accompanying with dark violet pigment on PDA. The optimal temperature and pH for the growth of the species was $25^{\circ}C$ and pH 7, respectively. Microscopic observation of one of isolates of the species shows that its conidiophores are unbranched and monophialides, its microconidia have oval-ellipsoidal shape with no septate and are of $3.0{\sim}11{\times}1.5{\sim}3.5\;{\mu}m$ sizes, its macroconidia are of $15{\sim}20{\times}2.0{\sim}3.5\;{\mu}m$ sizes and have slightly curved or slender shape with $2{\sim}3$ septate. The results of molecular analysis show that the ITS rDNA of F. oxysporum from paprika shares 100% sequence identity with that of known F. oxysporum isolates. The identified species proved it's pathogenicity by causing rotting symptom when it was inoculated on paprika fruits. The growth of F. oxysporum from paprika was suppressed on PDA by agrochemicals such as benomyl, tebuconazole and azoxystrobin. The identified species has the ability of producing extracelluar enzymes that degrade cellobiose and pectin.

Keywords

References

  1. Anonymous. 1960. Index of Plant Diseases in the United States. U.S. Dept. Agric. Handbook, No. 165. Washington, D.C. 531 p
  2. Alfieri, S. A., Jr., Langdon, K. R., Wehlberg, C. and Kimbrough, J. W. 1984. Index of Plant Diseases in Florida. Florida Dept. of Agric & Consumer Services, Div. of Plant Industry. Bull. No. 389 p
  3. Booth, C. 1970. Fusarium oxysporum. CMI descriptions of pathogenic fungi and bacteria. Kew, Surey, England
  4. Booth, C. 1971. The Genus Fusarium. Commonwealth Mycological Institute, Kew, Surrey, United Kingdom
  5. Choi, H. S., Ko, S. J., Kim, M. K., Park, J. W., Lee, S. H., Kim, K. H., Were, H. K., Choi, J.-K. and Takanami, Y. 2005. Characterization of Potato virus Y from paprika in Korea. Plant Pathol. J. 21: 349-354 https://doi.org/10.5423/PPJ.2005.21.4.349
  6. Farr, D. F., Bills, G. F., Chamuris, G. P. and Rossman, A. Y. 1989. Fungi on plants and plant products in the United States 5th edition, The American Phytopathological Society, Minnesota, USA
  7. Grand, L. F. 1985. North carolina plant disease index. North Carolina Agric. Res. Serv. Tech. Bull. 240: 1-157
  8. Jee, H. J., Ryu, K. Y., Shim, C. K. and Nam, K. W. 2005. Occurrence of stem and fruit rot paprika caused by Nectria haematococca. Plant Pathol. J. 21: 317-321 https://doi.org/10.5423/PPJ.2005.21.4.317
  9. Jeon, Y. J., Kwon, H. W., Nam, J. S. and Kim, S. H. 2006. Characterization of Sclerotinia sclerotiorum isolated from Paprika. Mycobiology 34: 154-157 https://doi.org/10.4489/MYCO.2006.34.3.154
  10. Joffe, A. Z. and Palti, J. 1977. Species of Fusarium found in uncultivated desert-type soils in Israel. Phytoparasitica 5: 119-122 https://doi.org/10.1007/BF02981151
  11. Leslie, J. F. and Summerell, B. A. 2006. The Fusarium Laboratory Manual. In: Identification strategies: The special case of Fusarium oxysporum, p. 109. Blackwell Publishing, Iowa, USA
  12. Kim, J. H., Choi, G. S. and Choi, J. K. 2002. Characterization of Cucumber mosaic virus subgroup II isolated from paprika (Capsicum annuum var. grossum), in Korea. Plant Pathol. J. 18: 6-11 https://doi.org/10.5423/PPJ.2002.18.1.006
  13. Kim, J.-H., Choi, G. S., Kim, J.-S. and Choi, J.-K. 2004. Characterization of Tomato spotted wilt virus from paprika in Korea. Plant Pathol. J. 20: 297-301 https://doi.org/10.5423/PPJ.2004.20.4.297
  14. Kim, S. H., Uzunovic, A. and Breuil, C. 1999. Rapid detection of Ophiostoma piceae and O. querqus in stained wood by PCR. Appl. Environ. Microbiol. 65: 187-190
  15. Kommedahl, T., Abbas, H. K., Burnes, P. M. and Mirocha, C. J. 1988. Prevalence and toxigenicity of Fusarium spp. from soils of Norway near the Arctic Circle. Mycologia 80: 790-794 https://doi.org/10.2307/3807556
  16. Leyendecker, P. J. and Nakayama, R. M. 1956. 1955 plant disease summary for New Mexico. Pl. Dis. Reporter 40: 159-161
  17. Link, H. F. 1809. Observations in ordines plantarum naturals, Dissetatio I. Mag. Ges. Naturf. Freunde. Berlin 3: 3-42
  18. Mandeel, Q. A., Abbas, J. A. and Saeed, A. M. 1995. Survey of Fusarium species in an arid environment of Bahrain: II. Spectrum of species on five isolation media. Sydowia 47: 223-239
  19. McMullen, M. P. and Stack, R. W. 1983. Fusarium species associated with grassland soils. Can. J. Bot. 61: 2530-2538 https://doi.org/10.1139/b83-277
  20. McMullen, M. P. and Stack, R. W. 1984. The effects of surface mining and reclamation on Fusarium populations of grassland soils. Reclamation and Revegetation Research 2: 253-266
  21. The Korean Society of Plant Pathology. 2004. List of plant diseases in Korea 4th edition, p. 172. Jung Hang Publishing, Seoul, Korea
  22. Raabe, R. D., Conners, I. L. and Martinez, A. P. 1981. Checklist of Plant Diseases in Hawaii. Hawaii Institute of Tropical Agriculture and Human Resource. College of Tropical Agriculture and Human Resources, University of Hawaii. Information Text Series no. 22. 313 p
  23. White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocol: a guide to methods and applications, ed. by M. A. Innis, D. H. Gelfand and J. J. Sninsky, Pp 315-322. Academic Press, London, UK

Cited by

  1. In vitro inhibitory effect of selected fungicides on mycelial growth of ambrosia fungus associated with the black coffee twig borer, Xylosandrus compactus Eichhoff (Coleoptera: Curculionidae) in Uganda vol.10, pp.23, 2015, https://doi.org/10.5897/AJAR12.1705
  2. Environmental effects on growth and sporulation of Fusarium spp. causing internal fruit rot in bell pepper vol.149, pp.4, 2017, https://doi.org/10.1007/s10658-017-1235-4
  3. in ginger pp.1465-3443, 2017, https://doi.org/10.1080/09540105.2017.1365820