Mycobiology

  • 제35권2호
  • /
  • Pages.76-81
  • /
  • 2007
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

Inhibition of Aflatoxin Production of Aspergillus flavus by Lactobacillus casei

  • Chang, In-Jeong (Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University) ;
  • Kim, Jeong-Dong (Institute of Industrial Biotechnology, Department of Biological Engineering, Inha University)
  • 발행 : 2007.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Lactobacillus casei KC-324 was tested for its ability to inhibit aflatoxin production and mycelial growth of Aspergillus flavus ATCC 15517 in liquid culture. flatoxin $B_{1}$ biosynthesis and mycelial growth were inhibited in both simultaneous culture and individual antagonism assays, suggesting that the inhibitory activity was due to extracellular metabolites. produced in cell-free supernatant fluids of the cultured broth of L. casei KC-324. In cell-free supernatant fluids of all media tested, deMan, Rogosa and Sharpe broth, potato dextrose broth, and Czapek-Dox broth+1% yeast extract showed higher antiaflatoxigenic activity. In these case, fungal growths, however, was not affected as measured by mycelial dry weight. The antiaflatoxigenic metabolites from L. casei KC-324 were produced over wide range of temperatures between $25^{\circ}C$ and $37^{\circ}C$. However, these metabolites were not thermostable since the inhibitory activity of the supernatant was inactivated within 30 minutes at $100^{\circ}C$ and $121^{\circ}C$. The inhibitory activity was not influenced by changing pH of supernatant between 4 and 10. However, the antiaflatoxigenic activity was slightly reduced at pH 10.

키워드

참고문헌

  1. Batish, V. K., Lal, R. and Grover, S., 1990. Studies of environmental and nutritional factors on production of antifungal substance by Lactobacillus acidophilus R. Food Microbiol. 7: 199-206 https://doi.org/10.1016/0740-0020(90)90025-D
  2. Campbell, T. C. and Stoloff, L. 1974. Implications of mycotoxins for human health. J. Agric. Food Chem. 22: 1006-1015 https://doi.org/10.1021/jf60196a016
  3. Choudhary, A. K. 1992. Influence of microbial co-inhabitants on aflatoxin synthesis of Aspergillus flavus on maize kernels. Lett. Appl. Microbiol. 14: 143-147 https://doi.org/10.1111/j.1472-765X.1992.tb00670.x
  4. Coallier-Ascah, J. and Idriak, E. E. 1985. Interaction between Streptococcus lactis and Aspergillus flavus on production of aflatoxin. Appl. Envirn. Microbiol. 49: 163-167
  5. Dantigny, P. and Molin, P. 2000. Influence of the modeling approach on the estimation of the minimum temperature for growth in Bilehraedek-type models. Food Microbiol. 17: 597-604 https://doi.org/10.1006/fmic.2000.0355
  6. Eaton, D. L. and Callagher, E. P. 1994. Mechanisms of aflatoxin carcinogenesis. Ann. Rev. Pharmacol. Toxicol. 34: 135-172 https://doi.org/10.1146/annurev.pa.34.040194.001031
  7. El-Nezami, H., Kankaanpaa, P., Salminen, S. and Ahokas, J. 1998. Ability of dairy strains of lactic acid bacteria to bind a common food carcinogen, aflatoxin $B_{1}$. Food Chem. Toxicol. 36: 321-326 https://doi.org/10.1016/S0278-6915(97)00160-9
  8. Fan, J. J. and Chen, J. H. 1999. Inhibition of aflatoxin-producing fungi by Welsh onion extracts. J. Food Prot. 62: 414-417 https://doi.org/10.4315/0362-028X-62.4.414
  9. Farr, D. F., Bills, G. F., Chamuris, G. P. and Rossman, A. Y. 1989. In Fungi on Plants and Plant Products in the United States, American Phytopathological Society Press, St. Paul, MN, USA
  10. Gournama, H. 1997. Inhibition of growth of mycotoxin production of Penicillium by Lactobacillus species. Food Sci. Technol.-LWT 30: 279-283 https://doi.org/10.1006/fstl.1996.0183
  11. Gournama, H. and Bullerman, L. B. 1995. Aspergillus flavus and Aspergillus parasiticus: aflatoxigenic fungi of concern in foods and feeds: a review. J. Food Protect. 58: 1395-1404 https://doi.org/10.4315/0362-028X-58.12.1395
  12. Han, E. M., Park, H. R., Hu, S. J., Kwon, K. S., Lee, H., Ha, M. S., Kim, K. M., Ko, E. J., Chun, H. S., Chung, D. H. and Bae, D. H. 2006. Monitoring of aflatoxin $B_{1}$ in livestock feeds using ELISA and HPLC. J. Microbiol. Biotechnol. 16: 643-646
  13. Karunaratne, A., Wezcnberg, E. and Bullerman, L. B. 1990. Inhibition of mold growth and aflatoxins production by Lactobacillus spp. J. Food Prot. 53: 230-236 https://doi.org/10.4315/0362-028X-53.3.230
  14. Kim, J.-D. 2005. Antifungal activity of lactic acid bacteria isolated from Kimchi against Aspergillus fumigatus. Mycobiology 33: 210-214 https://doi.org/10.4489/MYCO.2005.33.4.210
  15. Kimura, N. and Hiram, S. 1988. Inhibitory strains of Bacillus subtilis for growth and aflatoxin production of aflatoxigenic fungi. Agric. Biol. Chem. 52: 1173-1179 https://doi.org/10.1271/bbb1961.52.1173
  16. Klich, M. A. and Pitt, J. I. 1988. Differentiation of Aspergillus flavus from A. parasiticus and other closely related species. Trans. Br. Mycol. Soc. 91: 99-108 https://doi.org/10.1016/S0007-1536(88)80010-X
  17. Leifert C., Li, H., Siripun, C., Hampson, S., Workman, S., Sigee, D., Epton, H. A. S. and Harbour, A. 1995. Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45. J. Appl. Bacteriol. 78: 97-108 https://doi.org/10.1111/j.1365-2672.1995.tb02829.x
  18. Magnusson, J. and Schnurer, J. 2001. Lactobacillus coryniformis subsp. coryniformis strain Si3 produces a broad-spectrum proteinaceous antifungal compound. Appl. Environ. Microbiol. 67: 1-5 https://doi.org/10.1128/AEM.67.1.1-5.2001
  19. Munimbazi, C. and Bullerman, L. B. 1998. Inhibition of aflatoxin production of Aspergillus parasiticus NRRL 2999 by Bacillus pumilus. Mycopathologia 140: 163-169 https://doi.org/10.1023/A:1006832827266
  20. Muyncka, C. D., Leroya, A. I. J., Maeseneirea, S. D., Arnautb, F., Soetaerta, W. and Vandammea, E. J. 2004. Potential of selected lactic acid bacteria to produce food compatible antifungal metabolites. Microbiol. Res. 159: 339-346 https://doi.org/10.1016/j.micres.2004.07.002
  21. Paster, N., Juven, B. J. and Harshemesh, H. 1988. Antimicrobial activity and inhibition of aflatoxin $B_{1}$ formation by olive plant tissue constituents. J. Appl. Bacteriol. 64: 293-297 https://doi.org/10.1111/j.1365-2672.1988.tb01874.x
  22. Rasooli, I. and Razzaghi-Abyaneh, M. 2004. Inhibitory effects of Thyme oils on growth and aflatoxin production by Aspergillus parasiticus. Food Control 15: 479-483 https://doi.org/10.1016/j.foodcont.2003.07.002
  23. Razzaghi-Abyaneh, M., Allameh, A., Tiraihi, T., Shams-Ghahfarokhi, M. and Ghorbanian, M. 2005. Morphological alterations intoxigenic Aspergillus parasiticus exposed to neem (Azadirachta indica) leaf and seed aqueous extracts. Mycopathologia 159: 565-570 https://doi.org/10.1007/s11046-005-4332-4
  24. Squire, R. A. 1981. Ranking animal carcinogens: a proposed regulatory approach. Science 214: 877-881 https://doi.org/10.1126/science.7302565
  25. van Egmond, H. P. 1995. Mycotoxins: regulations, quality assurance and reference materials. Food Addit. Contam. 12: 321-330 https://doi.org/10.1080/02652039509374309

피인용 문헌

  1. vol.36, pp.1, 2008, https://doi.org/10.4489/MYCO.2008.36.1.013
  2. Reduction of Aflatoxin B1 Toxicity by Lactobacillus plantarum C88: A Potential Probiotic Strain Isolated from Chinese Traditional Fermented Food “Tofu” vol.12, pp.1, 2017, https://doi.org/10.1371/journal.pone.0170109
  3. Exploration, antifungal and antiaflatoxigenic activity of halophilic bacteria communities from saline soils of Howze-Soltan playa in Iran pp.1433-4909, 2017, https://doi.org/10.1007/s00792-017-0979-2
  4. Functional role of selenium-fortified yogurt against aflatoxin-contaminated nuts in rats vol.7, pp.1, 2018, https://doi.org/10.1186/s40066-018-0171-7
  5. Lactobacillus bulgaricus or Lactobacillus rhamnosus Suppresses NF-κB Signaling Pathway and Protects against AFB1-Induced Hepatitis: A Novel Potential Preventive Strategy for Aflatoxicosis? vol.11, pp.1, 2019, https://doi.org/10.3390/toxins11010017