Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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EXPRESSION OF TGF-${\beta}1$, OSTEONECTIN AND BMP-4 ON BONE REGENERATION IN MANDIBULAR DISTRACTION OSTEOGENESIS COMBINATION WITH A COMPRESSION STIMULATION

하악골 신장술에서 압축자극을 통한 골재생시 TGF-${\beta}1$, Osteonectin 및 BMP-4의 발현

  • Park, Seong-Jin (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Kim, Uk-Kyu (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Chung, In-Kyo (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Hwang, Dae-Seok (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Kim, Yong-Deok (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Shin, Sang-Hun (Dept. of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University) ;
  • Kim, Cheol-Hoon (Dept. of OMS, Dong-A Medical Center) ;
  • Byun, June-Ho (Dept. of OMS, Gyeongsang National University)
  • 박성진 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 김욱규 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 정인교 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 황대석 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 김용덕 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 신상훈 (부산대학교 치과대학 구강악안면외과학교실) ;
  • 김철훈 (동아의료원 구강악안면외과) ;
  • 변준호 (경상대학교 의과대학 구강악안면외과)
  • Published : 2007.08.31
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Abstract

Distraction osteogenesis(DO) is a technique of lengthning bone including soft tissue by gradual separation of surgically divided bone surfaces. Distraction osteogenesis combination with a compression stimulation(DO-CO) was a new technique by authors to enhance new bone quality and to shorten the consolidation period. The purpose of this study was to compare DO with DO combined with compression force in efficiency by evaluating the expression of TGF-${\beta}1$, osteonectin and BMP-4 on bone regenerate in rabbit mandible. Fourty two rabbits were used for this experiment. On the control group, the distraction was carried out at the rate of 1 mm per day to obtain the amount of 8 mm distraction for 8 days. On the experimental group, the distraction was carried out at the rate of 1 mm per day for 10 days, 3 days-latency period, and then the compression was carried out as counter direction 1 mm per day for 2 days. After 0 day, 5 days, 13 days, 20 days, 27 days, 34 days and 41 days, three rabbits on each group were sacrificed and the distracted portion of mandible were cut and treated for RT-PCR observation. The level of expression of TGF-${\beta}1$ and osteonectin were shown more and longer expression in the experimental group than in the control group. The expression of BMP-4 was maintained with high level during the entire experimental period in both groups. These findings suggested that DO with compression stimulation could be a favorable technique for obtaining a good new bone quality.

Keywords

References

  1. Codivilla A: On the means of lengthening in the lower limb, the muscles and tissues which are shortened through deformity. Am J Orthop Surg 1905;2:353-369
  2. Ilizarov GA: A new principle of osteosynthesis with the use of crossing pins and rings. In Collection of Scientific Works of the Kurgan Regional Scientific Medical society. Kurgan, USSR 1954:145-160
  3. Ilizarov GA: The tension-stress effect on the genesis and growth of tissues: I, The influence of stability of fixation and soft-tissue preservation. Clin Orthop 1989:238-249
  4. Ilizarov GA: The tension-stress effect on the genesis and growth of tissues: II, The influence of the rate and frequency of distraction. Clin Orthop 1989:239-263
  5. Snydr CC, Levine GA, Swanson HM, Browne EZ: Mandibular lengthening by gradual distraction. Plast Reconstr Surg 1973;51:506-508 https://doi.org/10.1097/00006534-197305000-00003
  6. McCarthy JG, Schreiber J, Karp NS, Throne CH, Grayson BH : Lengthning the human mandible by gradual distraction. Plast Reconstr Surg 1992;89:1-10 https://doi.org/10.1097/00006534-199289010-00001
  7. Pepper JR, Herbert MA, Anderson JR, et al: Effect of capacitive coupled electrical stimulation on regenerate bone. J Orthop Research 1996;14:296 https://doi.org/10.1002/jor.1100140219
  8. Greenwald JA, Luchs JS, Mehrara BJ, et al: 'Pumping the tegenetate': an evaluation of oscillating distraction osteogenesis in the rodent mandible. Ann Plast Surg 2000;44:516 https://doi.org/10.1097/00000637-200044050-00010
  9. Mizumoto Y, Moseley T, Timothy BS, et al: Acceleration of tegenerate ossification during distraction osteogenesis with tecombinant human bone morphogenetic protein-7. J Bone Joint Surg Am 2003;85:124
  10. Mofid MM, Inoue N, Atabey A, Marti G, Chao EY, Manson PN, Vandrt Kolk CA: Callus stimulation in distraction osteogenesis. Plast Reconstr Surg 2002 Apr;15:109:1620-1629
  11. Sakurakichi K, Tsuchiya H, Uehara K, Yamashiro T, Tomita K, Qzuma Y: Effect of timing of low-intensity pulsed ultrasound on distraction osteogenesis. J Orthop Res 2004;Mar:22(2):395-403 https://doi.org/10.1016/S0736-0266(03)00181-5
  12. Claes L, Rutter A, Mayer E: Low-intensity ultrasound enhances maturation of callus after segmental transport. Ciln Orthop Relat Res 2005;Jan:430:189-94
  13. 김욱규, 이광호, 신상훈, 정인교, 박봉수: 백서 하악골에서 신연골 형성술시 신장력과 수축력 복합적용후의 조직반응. 대한구강악안면외과학회지 2002;28:103
  14. 김욱규, 신상훈, 정인교, 김철훈, 허준, 윤일: 수축력과 신장력을 병용한 골신장술에서의 다양한 힘의 비와 부여시기에 따른 신연골 반응. 대한악안면성형재건외과학회지 2005;27:403
  15. Marie-Pierre Hello, Le Graverand, Carol Reno et al: Gene expression in menisci from knees of skeletally immature and mature female rabbits. Orthop Res 1999;17:738
  16. Morone MA, Boden SD, Hair G: Gene expression during autograft lumbar spine fusion and the effect of Bone Morphogenetic Protein 2. Clin Orthop 1998;351:252-265
  17. Delloye C, Delefortrie G, Coutelier L, Vincent A: Bone regenerate formationin cortical bone during distraction lengthning. An experimental study. Clin Orthop 1990;250:34-42
  18. Yasui N, Kojimoto H, Sasaki K, Kitada A, Shinomura Y: Factors affecting callus distraction in limb lengthning. Clin Orthop 1993;293:55-60
  19. White SH, Kenwright J: The timing of distraction of an osteotomy. J Bone Joint Surg Br 1990;72:356-361
  20. White SH, Kenwright J: The importance of delay in distraction of osteotomies Orthop Clin North Am 1991;22:569-579
  21. Tavakoli K, Walsh WR, Bonar F, Smart R, Wulf S, Poole MD: The role of latency in mandibular osteodistraction. J Craniomaxillofac Surg 1998;26:209-219 https://doi.org/10.1016/S1010-5182(98)80016-4
  22. Aronson J, Shen XC, Skinner RA, Hogue WR, Badger TM, Lumpkin CK Jr: Rat model of distraction osteogenensis. J Orthop Res 1997;15:221-226 https://doi.org/10.1002/jor.1100150210
  23. Stewart KJ,Lvoff GO,White AS, Bonar SF, Wslsh WR, Smart RC, er al: Mandibular distractio osteogenesis: A comparision of distraction rates in the rabbit model. J Craniomaxillofac Surg 1998;26:43-49 https://doi.org/10.1016/S1010-5182(98)80034-6
  24. 신선아, 지유진, 송현철: 가토하악골에서 신연 골형성술시 신연속도에 따른 $TGF-{\beta}1$. IGF-I, bFGF의 발현. 대한악안면성형재건외과학회지 2005;27:205-217
  25. Wolff J: The law of bone remodeling. Orthopade, 1995;24:378
  26. Kojimoto H, Yasui N, Goto T, Matsuda S, Shimomura Y: Bone lengthning in rabbit by callus distraction. J Bone Joint Surg Br 1988;70B:543-549
  27. Yasui N, Sato M, Ochi T, Kimura T, Kawahata H, Nomura S: Three modes of ossification during distraction osteogenesis in the rat. J Bone Joint Surg Br 1997;79:5:824-830 https://doi.org/10.1302/0301-620X.79B5.7423
  28. Buckley MJ, Banes AJ, Jordan RD: The effects of mechanical strain on osteoblasts in vitro. J Oral Maxillofac Surg 1990;48:276-282 https://doi.org/10.1016/0278-2391(90)90393-G
  29. Burger EH, Klein-Nulend J, Veldhuijzen JP: Mechanical sterss and steogenesis in vitro. J Bone Miner Res. Suppl 1992;2:S397-401
  30. Yeung HY, Lee KM, Fung KP, Leung KS: Substained expression of transforming growth factor-beta 1 by distraction during distraction osteogenesis in vitro. Life Sci 2002;24:71(1):67-79 https://doi.org/10.1016/S0024-3205(02)01575-8
  31. Rowe NM, Mehrara BJ, Dudziak ME, Steinbrech DS, Mackool RJ, Gittes GK et al: Rat mandibular distraction osteogenesis : Part I. Histologic and radiographic analysis. Plast Reconstr Surg 1998; 102:2022-2032 https://doi.org/10.1097/00006534-199811000-00033
  32. Mehrara BJ, Rowe NM, Steinbrech DS,Dudziak ME, Saadeh PB, McCarthy JG et al: Rat mandibular distraction ostegenesis: II, Molecular analysis of transforming growth factor beta 1 and osteocalcin gene expression. Plast Reconstr Surg 1999;103:536-547 https://doi.org/10.1097/00006534-199902000-00026
  33. 송정호, 신상훈, 김종렬: 백서 하악골에서 신연빈도에 따른 골형성 비교연구. 대한악안면성형재건외과학회지 2002;24:2:115-125
  34. Hiltunen A, Hannu TA, Vuorio E: Regulation of extracellular matrix genes during fracture healing. Clin Orthop 1993;297:23-27
  35. Meyer U, Meyer T, Vosshans J, Joos U: Decreased expression of osteocalcin and osteonectin in relaion to high strains and decreased mineralization in mandibular distraction osteogenesis. J Craniomaxillofac Surg 1999;27:222-227 https://doi.org/10.1016/S1010-5182(99)80033-X
  36. 백선호, 안병근, 박영주, 박희건, 박준우, 이건주 외: 성견 하악골 절단 후 기계적 골견인에 의해 형성된 신장부에 대한 시기별 조직학적 변화. 대한구강악안면외과학회지 2001;27:5:404-413
  37. 김동준, 지유진, 송현철: 가토하악골에서 신연 골형성술시 osteonectin의 발현. 대한악안면성형재건외과학회지 2004;30:5:391-399
  38. Urist MR: Bone formation by autoinduction. Science 1965;150:893-899 https://doi.org/10.1126/science.150.3698.893
  39. James H, Beaty MD Editor. Orthopaedic knowledge update, 1993;30-32
  40. 조태준, 최인호, 정진엽: 신연골 형성술과 골절 치유에서 제2형 및 제4형 골형성 단백 유전자 mRNA의 시간적, 공간적 발현. 대한정형외과학회지 1998;33:595-603
  41. Li G, Berven S, Simpson H, Triffitt JT: Expression of BMP-4 mRNA during distraction osteogenesis in rabbits. Acta Orthop Scand 1998;69:42-45