Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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ANTICANCER EFFECTS OF CAESALPINIA SAPPAN EXTRACTS ON ORAL CARCINOMA AND OSTEOSARCOMA CELLS

소목 추출물의 구강암 및 골육종 세포주에 대한 항암작용에 관한 연구

  • Lee, Jong-Su (Dept. of Oral Maxillofacial Surgery, School of Dentistry, Kyung Hee University) ;
  • Kim, Jeong-Hee (Dept. of Oral Biochemistry, School of Dentistry and Institute of Oral Biology, KyungHee University) ;
  • Kim, Yeo-Gab (Dept. of Oral Maxillofacial Surgery, School of Dentistry, Kyung Hee University)
  • 이종수 (경희대학교 치과대학 치의학과 구강악안면외과학교실) ;
  • 김정희 (경희대학교 치과대학 치의학과 구강생화학교실 및 구강생물학연구소) ;
  • 김여갑 (경희대학교 치과대학 치의학과 구강악안면외과학교실)
  • Published : 2007.12.31
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Abstract

Anticancer effect of methanol extract of Caesalpinia sappan L. on oral carcinoma(KB) and osteosarcoma(HOS) cells were investigated in this study. In order to elucidate the anticancer mechanism of Caesalpinia sappan L, we analyzed telomerase inhibitory effect of the methanol extract of Caesalpinia sappan L. In addition, we prepared 5 fraction samples according to its polarity differences and analyzed anticancer effects on oral carcinoma and osteosarcoma cells. Following results are obtained in this study. 1. 50% cell proliferation inhibitory value($IC_{50}$) of the methanol extract of Caesalpinia sappan L. against oral carcinoma(KB) cells and osteosarco ma(HOS) cells were $9.0{\mu}g/ml\;and\;10.9{\mu}g/ml$, respectively. 2. The methanol extract of Caesalpinia sappan L. showed inhibitory effect of telomerase which is required for cancer cell immortality. Therefore, it seems that the anticancer effect of methanol extract of Caesalpinia sappan is at least partially due to telomerase inhibitory effect. 3. Five fraction samples were prepared according to its polarity and 88.7% of ingredient of total methanol extract was transferred to ethylacetate fraction. Thin layer chromatography analysis showed that dichloromethane fraction contained ingredient with relatively high polarity and ethylacetate fraction contained similar ingredient found in total methanol extract. 4. Anticancer effect was observed in n-hexane, dichloromethane, and ethylacetate fractions. The highest anticancer effect was found in dichloromethane fraction which had $IC_{50}$ value of $4.4{\mu}g/ml$ and > $4.0{\mu}g/ml$ against oral carcinoma(KB) cells and osteosarcoma(HOS) cells, respectively.

Keywords

References

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