Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Effect of fur on pyrC Gene Expression

  • Chai, Sang-Ho (Department of Biomedical Sciences, Hallym University) ;
  • Song, Chang-Kyu (Department of Biomedical Sciences, Hallym University) ;
  • Kim, Seong-Kwun (Department of Biomedical Sciences, Hallym University) ;
  • Park, Jun-Ho (Department of Biomedical Sciences, Hallym University) ;
  • Wee, Se-Chan (Department of Biomedical Sciences, Hallym University)
  • Published : 2007.12.31
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Abstract

The promoter region of pyrC (dihydroorotase) gene of Escherichia coli was shown to have Fur protein binding properties by gel retardation assay. In vivo regulation of the pyrC expression was studied by measuring dihydroorotase activity and ${\beta}$-galactosidase level in the $fur^+$ and $fur^-$ genetic background. The expression of chromosomal dihydroorotase activity and ${\beta}$-galactosidase activity of pyrC-lacZ fusion plasmid was repressed to about 30% and 17%, respectively in the $fur^+$ strain compared to those in the $fur^-$ strain. Divalent ions such as $Fe^{2+}$ and $Zn^{2+}$ were not required for the repression. PyrC expression was also reduced to one half by 1 mM uracil. The effect of uracil was independent on the fur gene.

Keywords

References

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