Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Antioxidant Effect of Cynomorii Herba on HepG2 Cells and Diphenyl-picryl-hydrazyl (DPPH) Radical Scavenging Activity

쇄양(鎖陽)의 Diphenyl-picryl-hydrazyl (DPPH) 소거 활성 및 HepG2 세포에 대한 항산화 효과

  • Chang, Mun-Seog (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Yang, Woong-Mo (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Do-Rim (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Park, Eun-Hwa (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Park, Soo-Yeon (College of Physical education, Kyung Hee University) ;
  • Park, Seong-Kyu (Dept. of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
  • 장문석 (경희대학교 한의과대학 처방제형학교실) ;
  • 양웅모 (경희대학교 한의과대학 처방제형학교실) ;
  • 김도림 (경희대학교 한의과대학 처방제형학교실) ;
  • 박은화 (경희대학교 한의과대학 처방제형학교실) ;
  • 박수연 (경희대학교 체육대학) ;
  • 박성규 (경희대학교 한의과대학 처방제형학교실)
  • Published : 2007.12.30
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Abstract

The purpose of this study was to investigate the anti-oxidant effect of Cynomorium songaricum. The extract of Cynomorii Herba was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, HepG2 cell viability and $H_2O_2$-induced cytotoxicity by a modified MTT assay. DPPH radical scavenging activity was measured after 30 minutes. The extract was tested by 1. 5, 10, 50, 100 and 500 ${\mu}g/ml$ concentrations. HepG2 cell viability by a modified MTT assay was measured in the concentrations of 10, 50, 100, 250, 500 ug/ml for 24 h. The results showed that the extract scavenged DPPH radical up to 52.2% with 50 ug/ml concentration. The extract did not reduced the cell viability and $H_2O_2-induced$ cytotoxicity (69.4%) was blocked by the extract in the concentrations of 50, 100, 250 and 500 ${\mu}g/ml$. In conclusion, the extract of Cynomorii Herba has potent antioxidant activity.

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