대한의생명과학회지 (Biomedical Science Letters)
- 제13권4호
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- Pages.361-368
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- 2007
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Improvement of Histopathological Sample Preparation by Employing Microwave Heating Method on Frozen Section Specimens
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안승주
(대구보건대학 임상병리과)
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Ahn, Seung-Ju
(Department of Clinical Pathology, Daegu Health College)
- 발행 : 2007.12.31
초록
Biological samples can be fixed either by chemical method by using chemical solution or physical methods by using heat treatment. The problem in traditional heat fixation is unsatisfactory quality due to uneven heat conduction in specimen and loss of inner cell contents. Chemical fixation method also bears several intrinsic problems like the limit in specimen size, time consumption in fixative impregnation, and loss of low molecular weight cell components. These factors deteriorate the quality of fixed specimen, thus limit the magnification and contrast of tissue pictures. Microwave heat has been reported to be a good alternative to current chemical methods to overcome these problem. In this study, we tried to introduce the microwave energy method to routine fixation work in hospital. We replaced chemical fixative with saline to provide moderate reaction condition, and used frozen section to reduce time for sample preparation. Temperature was measured at each experiment. The fixation of rat kidney tissue with 2.45 GHz electromagnetic wave and saline showed similar result to the control group fixed with traditional chemical method. Human tumor tissue fixed with 2.45 GHz electromagnetic in frozen section was improved in terms of histochemistry of PAS and immunohistochemistry of tumor marker like cytokeratin. Total turnaround time was reduced from