Removal of Heavy Metals by Cladophora sp. in Batch Culture: The Effect of Wet-mixed Solidified Soil (loess) on Bioremoval Capacities

  • Kim, Jin-Hee (Department of Biology, Kyungpook National University) ;
  • Lee, Kyung-Lak (Department of Biology, Kyungpook National University) ;
  • Kim, Sook-Chan (Institute of Public Health & Environment, Gyeongsangbuk Do) ;
  • Kim, Han-Soon (Department of Biology, Kyungpook National University)
  • Published : 2007.12.31

Abstract

The heavy metal removal capacity of filamentous green alga Cladophora sp. cultured together with wet-mixed solidified soil (loess) was tested. A Cladophora sp. was cultured for 5d, with added Chu No. 10 medium, in stream water contaminated by high concentration of heavy metals from a closed mine effluent. Heavy metal ion concentrations of the medium and in algal tissue were measured every day during the experiment. Dissolved metals (Al, Cd, Cu, Fe, Mn, Zn) in medium were rapidly removed (over 90% elimination) within 1-2d when alga and loess were added. Dissolved heavy metals dropped by only 10% when algae were cultured without loess. The Cladophora sp. accumulated much more heavy metals when cultured with loess than when the alga was cultured alone. Cladophora sp. exhibited a maximum uptake capacity for Al ($17,000{\mu}g^{-1}$ algal dry weight). The metal bioremoval capacities of the algae were in the order Al, Fe, Cu, Mn, Zn and Cd. The heavy metal removal capacity of Cladophora sp. showed significant increases when wet-mixed solidified soil was added to culture media.

Keywords

References

  1. Aksu, Z., D. Ozer, A. Ozer, T. Kutsal and A. Caglar. 1998. Investigation of the column performance of cadmium (II) biosorption by Cladophora crispate floes in a packed bed. Separat. Sci. Technol. 33: 667-682 https://doi.org/10.1080/01496399808544782
  2. Anderson, D.M. 1997. Turning back the harmful red tide. Nat. 388: 513-515 https://doi.org/10.1038/41415
  3. APHA. 1995. Standard methods for the examination of water and wastewater. American Public Health Association, Washington, DC
  4. Avnimelech, Y., B.W. Troeger and L.W. Leed. 1982. Mutual flocculation of algae and clay: Evidence and implications. Science 216: 63-65 https://doi.org/10.1126/science.216.4541.63
  5. Bae, H.M., H. Choi, W.C. Lee and S.J. Yoon. 1998. Control of the red tide by yellow loess dispersion. p. 53-60. In: Proceedings of the Korea-China joint symposium on harmful algal bloom
  6. Bux, F., B.W. Atkinson and H.C. Kasen. 1997. Laboratory and pilot-plant bioreactor development for remediation of metal-contaminated wastewater using activated sludge as biosorbent. Water Research Commission Report no. 688(1/97). Pretoria. p.97
  7. Chantana, L., K. Maleeya, P. Prayad, E.S. Upatham and S. Varasaya. 2005. Toxicity and accumulation of lead and cadmium in the filamentous green alga Cladophora fracta (O.F. Muller ex Vahl) Kutzing: A laboratory study. Science Asia 31: 121-127 https://doi.org/10.2306/scienceasia1513-1874.2005.31.121
  8. Gupta, V.K., A.K. Shrivastava and N. Jain. 2001. Biosorption of chromium (VI) from aqueous solutions by green algae Spirogyra species. Water Res. 35: 4079-4085 https://doi.org/10.1016/S0043-1354(01)00138-5
  9. Howell, B.R. and R.G.J. Shelton. 1970. The effect of China clay on the bottom faubna of St. Austell and Mevagissey bays. J. Mar. Biol. Ass. U.K. 50: 593-706 https://doi.org/10.1017/S0025315400004902
  10. Lasheen, M.R. 1990. Effect of cadmium, copper and chromium (VI) on the growth of Nile algae. Water Air Soil Pollut. 50: 19-30
  11. Leborans, G.F. and A Novillo. 1996. Toxicity and bioaccumulation of cadmium in Olishodiscus luteus (Raphidophyceae). Water Res. 30: 57-62 https://doi.org/10.1016/0043-1354(95)00084-X
  12. Lettermann, R.D. and W.J. Mitsch. 1978. Impact of mine drainage on a mountain stream in Pennsylvania. Environ. Pollut. 17: 53-73 https://doi.org/10.1016/0013-9327(78)90055-1
  13. Na, G., W. Choi and C. Yoon. 1996. A study on red tide control with loess suspension. J. Aquacult. 9: 239-245
  14. Nakajima, A. and T. Sakaguchi. 1986. Selective accumulation of heavy metals by microorganisms. Appl. Microbiol. Biotechnol. 24: 59-64
  15. Ozer, D., A. Ozer and G. Dursan. 2000 Investigation of zinc (II) adsorption on Cladophora crispata in a two-staged reactor. J. Chem. Technol. Biotechnol. 75: 410-416 https://doi.org/10.1002/(SICI)1097-4660(200005)75:5<410::AID-JCTB226>3.0.CO;2-X
  16. Portman, J.E. 1970. The effect of China clay on the sediments of St. Austell and Mevagissey bays. J. Mar. Biol. Ass. U.K. 50: 577-591 https://doi.org/10.1017/S0025315400004896
  17. Raskin, I., PBAN. Kumar, S. Dushenkov and D. Salt. 1994. Bioconcentration of heavy metals by plants. Curr. Opin. Biotechnol. 28: 115-126
  18. Roy, D., P.N. Greenlaw and B.S. Shane. 1992. Adsorption of heavy metals by green algae. J. Environ. Sci. Health A. 28: 37-50 https://doi.org/10.1080/10934529309375861
  19. Sahu, R.K., S. Katiyar, J. Tiwari and G.C. Kisku. 2007. Assessment of drain water receiving effluent from tanneries and its impact on soil and plants with particular emphasis on bioaccumulation of heavy metals. J. Environ. Biol. Supp. 28: 685-690
  20. Sengco, M.R., A. Li, K. Tugend, D. Kulis and D.M. Anderson. 2001. Removal of red and brown tide cells using clay flocculation: 1. Laboratory culture experiments with Gymnodinium breve and Aureococcus anophagefferens. Mar. Ecol. Prog. Ser. 210: 41-53 https://doi.org/10.3354/meps210041
  21. Singh, A., S.K. Mehta and J.P. Gaur. 2007. Removal of heavy metals from aqueous solution by common freshwater filamentous algae. World J. Microbiol Biotechnol. 23: 1115-1120 https://doi.org/10.1007/s11274-006-9341-z
  22. Sobhan, R. and S.P.K. Sternberg. 1999. Cadmium removal using Cladophora. J. Environ. Sci. Health A. 34: 53-72 https://doi.org/10.1080/10934529909376823
  23. Sternberg, S.P.K. and R.W. Dorn. 2002. Cadmium removal using Cladophora in batch, semi-batch and flow reactors. Bioresource Technology. 81: 249-255 https://doi.org/10.1016/S0960-8524(01)00131-6
  24. Volesky, B. and Z.R. Holan. 1995. Biosorption of he avy metals. Biotechnol. Prog. 11: 235-250 https://doi.org/10.1021/bp00033a001
  25. Wilde, E.W. and J.R. Benemann. 1993. Bioremoval of heavy metals by the use of microalgae. Biotech. Adv. 11: 781-812 https://doi.org/10.1016/0734-9750(93)90003-6
  26. Yu, Q., J.T. Matheickal, P. Yin and P. Kaewsarn. 1999. Heavy metal uptake capacities of common marine macro algal biomass. Water Res. 33: 1534-1537 https://doi.org/10.1016/S0043-1354(98)00363-7