생태와환경 (Korean Journal of Ecology and Environment)

  • 제40권4호
  • /
  • Pages.489-494
  • /
  • 2007
  • /
  • 2288-1115(pISSN)
  • /
  • 2288-1123(eISSN)
한국수생태학회 (The Korean Society of Limnology)
권호 표지

Effects of Acute Metal Exposures on the Viability and mRNA Expression of Metallothionein in Hemibarbus mylodon Fry

  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Cho, Young-Sun (Department of Aquaculture, Pukyong National University) ;
  • Lee, Il-Rho (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Nam, Yoon-Kwon (Department of Aquaculture, Pukyong National University)
  • 발행 : 2007.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Transcriptional modulation of metallothionein (MT) during acute metal exposures (cadmium, copper or zinc) was examined in fry of Hemibarbus mylodon, a threatened fish species in Korean peninsula. Viability of H. mylodon fry was most affected by copper exposure (up to 79% of mortality at 1 ppm for 48 hours) and considerably by cadmium exposure (21 to 54% of mortality). On the other hand, Zn showed the least adverse effect on the viability (0 to 13% of mortality) of this species. Based on the semi-quantitative RT-PCR analysis, the stimulation of MT mRNA in response to metal exposures followed generally in a dose-dependent fashion where cadmium was the most potent inducer for the induction of MT transcripts in fry (up to more than 5-fold) while the lowest response was observed in zinc-exposed group (2-fold at maximum). From the exposure using environmentally realistic doses of cadmium (0 to 0.05 ppm for 24 hours), MT expression at mRNA level was also sensitively modulated toward upregulation up to more than 3-fold as relative to non-exposed control. Results from the present study would be a good basis for understanding the adaptive capacity and stress physiology of this endangered fish species during metal pollution.

키워드

참고문헌

  1. Alexandra, L. and A. Hontela, 2004. A comparative assessment of the adrenotoxic effects of cadmium in two teleost species, rainbow trout, Oncorhynchus mykiss, and yellow perch, Perea flavescens. Aquat. Toxicol. 67: 13-21 https://doi.org/10.1016/j.aquatox.2003.11.010
  2. Alvarado, N.E., I. Quesada, K. Hylland, I. Marigomeza and M. Soto. 2006. Quantitative changes in metallothionein expression in target cell-types in the gills of turbot (Scophthalmus maxim us) exposed to Cd, Cu, Zn and after a depuration treatment. Aquat. Toxicol. 77: 64-77 https://doi.org/10.1016/j.aquatox.2005.10.017
  3. Basha, P.S. and A.U. Rani. 2003. Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus (Tilapia). Ecotoxicol. Environ. Saf. 56: 218-221 https://doi.org/10.1016/S0147-6513(03)00028-9
  4. Cho, Y.S., B.N. Choi, E.M. Ha, K.H. Kim, S.K. Kim, D.S. Kim and Y.K. Nam. 2005. Shark (Scyliorhinus torazame) metallothionein: cDNA cloning, genomic sequence, and expression analysis. Mar. Biotechnol. (NY) 7: 350-362 https://doi.org/10.1007/s10126-004-0043-y
  5. Cho, Y.S., B.N. Choi, K.H. Kim, S.K. Kim, D.S. Kim, I.C. Bang and Y.K. Nam. 2006. Differential expression of CU/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnathus fasciatus). Aquaculture 253: 667-679 https://doi.org/10.1016/j.aquaculture.2005.05.047
  6. Coyle, P., J.C. Philcox, L.C. Carey and A.M. Rofe. 2002. Metallothionein: the multipurpose protein. Cell. Mol. Life Sci. 59: 627-647 https://doi.org/10.1007/s00018-002-8454-2
  7. Dang, Z., M.H.G. Berntssen, A.K. Lundebye, G. Flik, S.E. Wendelaar Bonga and R.A.C. Lock. 2001. Metallothionein and cortisol receptors expression in gills of Atlantic salmon, Salmo salar, exposed to dietary cadmium. Aquat. Toxicol. 53:91-101 https://doi.org/10.1016/S0166-445X(00)00168-5
  8. De Boeck, G., T.T.H. Ngo, K.V. Campenhout and R. Blust. 2003. Differential metallothionein induction patterns in three freshwater fish during sublethal copper exposure. Aquat. Toxicol. 65: 413-424 https://doi.org/10.1016/S0166-445X(03)00178-4
  9. Grosell, M., J. Blanchard, K.V. Brix. and R. Gerdes. 2007. Physiology is pivotal for interactions between salinity and acute copper toxicity to fish and invertebrates. Aquat. Toxicol. 84: 162-172 https://doi.org/10.1016/j.aquatox.2007.03.026
  10. Haq, F., M. Mahoney and J. Koropatnick. 2003. Signaling events for metallothionein induction. Mutation Res. 533: 211-266 https://doi.org/10.1016/j.mrfmmm.2003.07.014
  11. Hermesz, E., M. Abraham and J. Nemcsok. 2001. Tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock. Comp. Biochem. Physiol. 128C: 457-465
  12. Jang, M.-H., M.C. Lucas and G.-J. Joo. 2003. The fish fauna of mountain streams in South Korean national parks and its significance to conservation of regional freshwater fish biodiversity. Biol. Conservation 114: 115-126 https://doi.org/10.1016/S0006-3207(03)00016-8
  13. Kim, K.Y., S.Y. Lee, Y.S. Cho, I.C. Bang, D.S. Kim and Y.K. Nam. 2007. Characterization and phylogeny of two B-cytoskeletal actins from Hemibarbus mylodon (Cyprinidae, Cypriniformes), a threatened fish species in Korea. DNA Seq. (in press; DOI: 10.1080/10425170701445691)
  14. Kock, G., R. Hoper and S. Wograth. 1995. Accumulation of trace metals Cd, Pb, Cu, Zn in arctic char Salvelinus alpinus from oligotrophic alpine lakes: relation to alkalinity. Can. J. Fish. Aquat. Sci. 52: 2367-2376 https://doi.org/10.1139/f95-829
  15. Matsuo, A.Y.O., C.M. Wood and A.L. Val. 2005. Effects of copper and cadmium on ion transport and gill metal binding in the Amazonian teleost tambaqui (Colossoma macropomum) in extremely soft water. Aquat. Toxicol. 74: 351-364 https://doi.org/10.1016/j.aquatox.2005.06.008
  16. McGeer, J.C., S. Nadella, D.H. Alsop, L. Hollis, L.N. Taylor, D.G. McDonald and C.M. Wood. 2007. Influence of acclimation and cross-acclimation of metals on acute Cd toxicity and Cd uptake and distribution in rainbow trout (Oncorhynchus myhiss). Aquat. Toxicol. 84: 190-197 https://doi.org/10.1016/j.aquatox.2007.03.023
  17. Meinelt, T. R.C. Playle, M. Pietrock, B.K. Burnison, A. Wienke and C.E.W. Steinberg. 2001. Interaction of cadmium toxicity in embryos and larvae of zebrafish (Danio rerio) with calcium and humic substances. Aquat. Toxicol. 54: 205-215 https://doi.org/10.1016/S0166-445X(01)00145-X
  18. Olsson, P-E., P. Kling and C. Hogstrand. 1998. Mechanisms of heavy metal accumulation and toxicity in fish. In: Langston, W.J., M.J. Bebiano (Eds.), Metal Metabolism in Aquatic Environments. Chapman and Hall, London, p. 321-350
  19. Olsvik, P.A., P. Gundersen, R.A. Andersen and K.E. Zachariassen. 2001. Metal accumulation and metallothionein in brown trout, Salmo trutta, from two Norwegian rivers differently contaminated with Cd, Cu and Zn. Comp. Biochem. Physiol. 128C: 189-201
  20. Pierron, F., M. Baudrimont, A. Bossy, J.P. Bourdineaud, D. Brethes, P. Elie and J.C. Massbuau. 2007. Impairment of lipid storage by cadmium in the European eel (Anguilla anguilla). Aquat. Toxicol. 81: 304-311 https://doi.org/10.1016/j.aquatox.2006.12.014
  21. Risso-de Faverney, C., A. Devaux, M. Lafaurie, J.P. Girad, B. Bailly and R. Rahmani. 2001. Cadmium induces apoptosis and genotoxicity in rainbow trout hepatocytes through generation of reactive oxygen sepecies. Aquat. Toxicol. 53: 65-76 https://doi.org/10.1016/S0166-445X(00)00154-5