Application of Pulsed Electric Fields with Square Wave Pulse to Milk Inoculated with E. coli, P. fluorescens, and B. stearothermophilus

  • Shin, Jung-Kue (Department of Korean Traditional Food Culture, Jeonju University) ;
  • Jung, Kwan-Jae (Department of Biotechnology, Yonsei University) ;
  • Pyun, Yu-Ryang (Department of Biotechnology, Yonsei University) ;
  • Chun, Myong-Soo (Department of Food Science and Technology, Ewha Womans University)
  • Published : 2007.12.31

Abstract

Ultra-high temperature (UHT) processed full fat milk inoculated with Escherichia coli, Pseudomonas fluorescens, and Bacillus stearothermophilus was exposed to 30-60 kV/cm square wave pulsed electric field (PEF) with $1\;{\mu}sec$ pulse width, and $26-210\;{\mu}sec$ treatment time in a continuous PEF treatment system. Eight log reduction was obtained for E. coli and P. fluorescens and 3 logs reduced for B. stearothermophilus under PEF treatment conditions of $210\;{\mu}sec$ treatment time, 60 kV/cm pulse intensity at $50^{\circ}$. There was no significant change in pH and titration acidity of milk after PEF treatment. The electrical energy required to achieve 8 log reduction for E. coli and P. fluorescens was estimated to be about 0.74 kJ/L.

Keywords

References

  1. Ho SY, Mittal GS, Cross JD, Griffiths MW. Inactivation of P. fluorescens by high voltage pulsed electric pulse. J. Food Sci. 60: 1337-1340 (1995) https://doi.org/10.1111/j.1365-2621.1995.tb04586.x
  2. Lubicki P, Jayaram S. High voltage pulse application for the destruction of the Gram-negative bacteria Yersinia enterocolitica. Bioelectroch. Bioener. 43: 135-141 (1997) https://doi.org/10.1016/S0302-4598(96)05153-7
  3. Mazurek B, Lubicki P, Staroniewicz Z. Effect of short duration HV pulses on bacteria and fungi. IEEE T. Dielect. El. In. 2: 418-425 (1995) https://doi.org/10.1109/94.395420
  4. Amiali M, Ngadi MO, Smith JP, Raghavan GSV. Synergistic effect of temperature and pulsed electric field on inactivation of Escherichia coli O157:H7 and Salmonella enteritidis in liquid egg yolk. J. Food Eng. 79: 689-694 (2007) https://doi.org/10.1016/j.jfoodeng.2006.02.029
  5. Fleischman GJ, Ravishankar S, Balasubramaniam VM. The inactivation of Listeria monocytogens by pulsed electric field (PEF) treatment in a static chamber. Food Microbiol. 21: 91-95 (2004) https://doi.org/10.1016/S0740-0020(03)00015-7
  6. Knorr D, Guelen M, Grahl T, Sitzmann W. Food application of high voltage electric field pulses. Trends Food Sci. Tech. 5: 71-75 (1994) https://doi.org/10.1016/0924-2244(94)90240-2
  7. Zhang Q, Barbosa-Canovas GV, Swanson BG. Inactivation of E. coli for food pasteurization by high-strength pulsed electric fields. J. Food Process Pres. 19: 103-118 (1995) https://doi.org/10.1111/j.1745-4549.1995.tb00281.x
  8. Evrendilek GA, Zhang Q, Richter ER. Application of pulsed electric fields to skim milk inoculated with Staphylococcus aureus. Biosyst. Eng. 87: 137-144 (2004) https://doi.org/10.1016/j.biosystemseng.2003.11.005
  9. Jayaram S, Castle GSP. Kinetics of sterilization of Lactobacillus brevis cells by the application on high voltage pulses. Biotechnol. Bioeng. 40: 1412-1420 (1992) https://doi.org/10.1002/bit.260401116
  10. Vega-Mercado H, Martin-Belloso O, Barbosa-Canovas GV, Swanson BG. Non-thermal food preservation (pulsed electric fields). J. Food Sci. Tech. Mys. 8: 151-157 (1997) https://doi.org/10.1016/S0924-2244(97)01016-9
  11. Qin BL, Barbosa-Canovas GV, Swanson BG. Inactivating microorganisms using a pulsed electric field continuous treatment system. IEEE T. Ind. Appl. 34: 43-50 (1998)
  12. Biziak RB, Swartzel KR, Jones VA. Energy use for continuous thermal processing of milk. J. Food Sci. 50: 1607-1614 (1985) https://doi.org/10.1111/j.1365-2621.1985.tb10544.x
  13. Chandarana DI, Frey BC, Stewart LE, Mattick JF. UHT milk processing effect on process energy requirements. J. Food Sci. 49: 977-978 (1984) https://doi.org/10.1111/j.1365-2621.1984.tb13262.x
  14. Grahl T, Markl H. Killing of microorganisms by pulsed electric fields. J. Appl. Microbiol. Biotechnol. 45: 148-157 (1996) https://doi.org/10.1007/s002530050663
  15. Hulsheger H, Pottel J, Niemann EG. Killing of bacteria with electric pulses of high field strength. Radiat. Environ. Bioph. 20: 53-65 (1981) https://doi.org/10.1007/BF01323926