Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Evaluation of the Estrogenic Activity by Yeast Two-hybrid Assay and Enzyme-linked Immunosorbent Assay in Sewage Treatment Plant

하수처리장의 내분비계장애물질에 대한 Yeast Two-hybrid Assay와 Enzyme-linked Immunosorbent Assay에 의한 에스트로겐활성도 평가

  • Lee, Byoung-Cheun (Department of Environment Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Ra, Jin-Sung (Department of Environment Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Kim, Sang-Don (Department of Environment Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Kawai, Hukiko (Huji Electric Advanced Technology Co., Ltd.) ;
  • Lee, Chul-Hee (Department of Environmental Engineering, Yeungnam University)
  • 이병천 (광주과학기술원 환경공학과) ;
  • 나진성 (광주과학기술원 환경공학과) ;
  • 김상돈 (광주과학기술원 환경공학과) ;
  • ;
  • 이철희 (영남대학교 건설환경공학부)
  • Published : 2007.07.31
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Abstract

Several endocrine disrupting chemicals(EDCs) were monitored to evaluate the estrogenic activities and the concentrations by yeast two-hybrid assay and enzyme-linked immunosorbent assay(ELISA) in sewage treatment plant(STP) which consist of industrial and domestic line. In the influent of domestic line, estrone, 17$\beta$-estradiol, 17$\alpha$-ethinylestradiol and alkylphenolethoxylate(APE) were detected up to 167.1, 39.7, 7.3 and 145.4 ng/L, respectively. The average removal efficiency of 17$\beta$-estradiol after the activated sludge process was 77.5% and further removed to 80.8% after the sand filtration-ozonation step. These results suggests that the activated sludge process has limited potential to remove the estrogenic activity effectively. The contributions of the estrogenic chemicals to the estrogenic activities were 70.7, 23.3, 3.7 and 2.3% for estrone, 17$\beta$-estradiol 17$\alpha$-ethinylestradiol and APE, respectively, in the domestic line effluents. Therefore, 17$\beta$-estradiol and estrone contributed most of the estrogenic activity in the domestic line effluents.

가정계열과 공단계열로 분리하여 처리되는 하수처리장에서 에스트로겐 활성을 평가하기 위하여 yeast two-hybrid assay와 enzyme-linked immunosorbent assay(ELISA)를 이용하여 내분비계장애물질의 농도와 활성도를 측정하였다. 그 결과 가정계열 유입수 중에서 estrone (E1), 17$\beta$-estradiol(E2), 17$\alpha$-ethinylestradiol(EE2) 그리고 APE의 농도는 각각 최대 167.1, 39.7, 7.3, 145.4 ng/L까지 검출되었다. 활성슬러지법에 의한 처리로, 17$\beta$-estradiol의 평균제거율은 77.5%, 고도처리 공정인 모래여과와 오존산화를 거친 후에는 80.8%까지 제거되는 것으로 나타났다. 동시에 Yeast two-hybrid assay로 각 내분비계장애물질의 농도-반응곡선으로부터 반응식을 구하여, 에스트로겐 활성에 미치는 각 물질의 기여도를 분석한 결과, 가정계의 활성슬러지법에 의한 처리수에서 estrone, 17$\beta$-estradiol 17$\alpha$-ethinylestradiol, APE 가 각각 70.7, 23.3, 3.7, 2.32%로 나타났다. 즉, 생물학적 처리공정을 통해 배출된 처리수의 에스트로겐 활성에 영향을 미치는 주된 기여물질은 estrone과 17$\beta$-estradiol인 것으로 나타났다.

Keywords

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