생명과학회지 (Journal of Life Science)

  • 제17권1호
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  • Pages.150-161
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  • 2007
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
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흰쥐 대뇌세포의 저산소증 모델에서 석창포(石菖浦 Acori graminei rhizoma. AGR)에 의한 유전자 표현 변화의 microarray 분석

Microarray Analysis of Alteration in Gene Expression by Acori graminei rhizoma (AGR) Water-Extract in a Hypoxic Model of Cultured Rat Cortical Cells

  • 박동준 (동국대학교 한의과대학 내과학교실) ;
  • 정승현 (동국대학교 한의과대학 내과학교실) ;
  • 문일수 (동국대학교 의과대학 해부학교실) ;
  • 이원철 (동국대학교 한의과대학 내과학교실) ;
  • 신길조 (동국대학교 한의과대학 내과학교실)
  • 발행 : 2007.01.29
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초록

Acori graminei Rhizomn (AGR) is a perennial herb which has been used clinically as a traditional oriental medicine against stroke, Alzheimer's disease, and vascular dementia. We investigated the effect of AGR on the modulation of gene expression profile in a hypoxic model of cultured rat cortical cells. Rat cerebrocortical cells were grown in Neurobasal medium. On DIV12, cells were treated with AGR $(10ug/m\ell)$, given a hypoxic shock (2% $O_2$, 3 hr) on DIV14, and total RNAs were prepared one day after shock. Microarray analyses indicated that the expression levels of most genes were altered within the global M values +0.5 and -0.5, i.e., 40% increase or decrease. There were 750 genes which were upregulated by < global M +0,2, while 700 genes were downregulated by > global M -0.2. The overall profile of gene expression suggests that AGR suppresses apoptosis (upregulation of anti-apopotic genes such as TEGT, TIEG, Dad, p53, and downregulation of pro-apopotic genes such as DAPK, caspase 2, pdcd8), ROS (upregulation of RARa, AhR), and that AGR has neurotrophic effects (upregulation of Aktl, Akt2). These results provide a platform for investigation of the molecular mechanism of the effect of AGR in neuroprotection.

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참고문헌

  1. Ashktorab, H., A. Ahmed, G. Littleton, X. W. Wang, C. R. Allen, R. Tackey, C. Walters and D. T. Smoot. 2003. p53 and p14 increase sensitivity of gastric cells to H. pylori-induced apoptosis, Dig Dis Sci. 48, 1284-1291 https://doi.org/10.1023/A:1024198807619
  2. Banasiak, K. J. and G. G. Haddad. 1998. Hypoxia-induced apoptosis: effect of hypoxic severity and role of p53 in neuronal cell death. Brain Res. 797, 295-304 https://doi.org/10.1016/S0006-8993(98)00286-8
  3. Barks, J.D. and F. S. Silverstein. 1992. Excitatory amino acids contribute to the pathogenesis of perinatal hypoxic-ischemic brain injury. Brain Pathol. 235-243
  4. Beilharz, E. J. and C. E. Williams, M. Dragunow, E. S. Sirmanne and P. D. Gluckman. 1995. Mechanisms of delayed cell death following hypoxic-ischemic injury in the immature rat: evidence for apoptosis during selective neuronal loss. Mol Brain Res. 29, 1-14 https://doi.org/10.1016/0169-328X(94)00217-3
  5. Besnard, S., C. Heymes, R. Merval, M. Rodriguez, J. P. Galizzi, J. A. Boutin, J. Mariani and A. Tedgui, 2002. Expression and regulation of the nuclear receptor RORalpha in human vascular cells. FEBS Lett. 511, 36-40 https://doi.org/10.1016/S0014-5793(01)03275-6
  6. Brewer, G. J., J. R. Torricelli, E. K. Evege and P. J. Price. 1993. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J. Neurosci Res. 35, 567-676 https://doi.org/10.1002/jnr.490350513
  7. Brewster, J.L., S. L. Martin, J. Toms, D. Goss, K. Wang, K. Zachrone, A. Davis, G. Carlson, L. Hood and J. D. Coffin. 2000. Deletion of Dad1 in mice induces an apoptosis-associated embryonic death. Genesis 26, 271-278 https://doi.org/10.1002/(SICI)1526-968X(200004)26:4<271::AID-GENE90>3.0.CO;2-E
  8. Chae, H. J., N. Ke, H. R. Kim, S. Chen, A. Codzik, M. Dickman and J. C. Reed. 2003. Evolutionarily conserved cytoprotection provided by Bax Inhibitor-1 homologs from animals, plants, and yeast. Gene 323, 101-113 https://doi.org/10.1016/j.gene.2003.09.011
  9. Chang, H. H. P. and P. H. But. 1986. In Pharmacology and Applications of Chinese Materia Medica vol. 1, pp. 334-337. World Scientific Publishing, Singapore
  10. Chauvet, C., B. Bois-Joyeux and J. L. Danan. 2002. Retinoic acid receptor-related orphan receptor(ROR) alpha4 is the predominant isoform of the nuclear receptor RORalpha in the liver and is up-regulated by hypoxia in HepG2 human hepatoma cells. Biochem J. 364, 449-456 https://doi.org/10.1042/BJ20011558
  11. Cho, J., N. E. Joo, J. Y. Kong, D. Y. Jeong, K. D. Lee and B. S. Kang, 2000. Inhibition of excitotoxic neuronal death by methanol extract of Acori graminei rhizoma in cultured rat cortical neurons. J. Ethnopharmacol. 73, 31-37 https://doi.org/10.1016/S0378-8741(00)00262-2
  12. Cho, J., Y. H. Kim, J. Y. Kong, C. H. Yang and C. G. Park, 2002. Protection of cultured rat cortical neurons from excitotoxicity by asarone, a major essential oil component in the rhizomes of Acorus gramineus. Life Sci. 71, 591-599 https://doi.org/10.1016/S0024-3205(02)01729-0
  13. Colantuoni, C., A. E. Purcell, C. M. Bouton and J. Pevsner. 2000. High throughput analysis of gene expression in the human brain. J. Neurosci Res. 59, 1-10 https://doi.org/10.1002/(SICI)1097-4547(20000101)59:1<1::AID-JNR1>3.0.CO;2-2
  14. Duggan, D. J., M. Bittner, Y. Chen, P. Meltzer and J. M. Trent. 1999. Expression profiling using cDNA microarrays. Nat Genet. 21, 10-14 https://doi.org/10.1038/4434
  15. Ebert, B. L., J. D. Firth, P. J. Ratcliffe. 1995. Hypoxia and mitochondrial inhibitors regulate expression of glucose transporter-1 via distinct Cis-acting sequences. J. Biol Chem. 270, 29083-29089 https://doi.org/10.1074/jbc.270.49.29083
  16. Friedman, J. E. and G. G. Haddad. 1993. Major differences in $Ca^{2+}$, response to anoxia between neonatal and adult rat CA1 neurons: role of $Ca^{2+}$ and $Na^+$. J. Neurosci. 13, 63-72 https://doi.org/10.1523/JNEUROSCI.13-01-00063.1993
  17. Fujio, Y., Y. Mitsuuchi, J. R. Testa and K. Walsh. 2001. Activation of Akt2 Inhibits anoikis and apoptosis induced by myogenic differentiation. Cell Death Differ. 8, 1207-1212 https://doi.org/10.1038/sj.cdd.4400919
  18. Goldberg M. A., S. P. Dunning and H. F. Bunn. 1988. Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein. Science. 242, 1412-1415 https://doi.org/10.1126/science.2849206
  19. Gonzalez, F.J., P. Fernandez-Salguero. 1998. The aryl hydrocarbon receptor: studies using the AHR-null mice. Drug Metab Dispos. 26, 1194-1198
  20. Gu, Y. Z., J. B. Hogenesch, C. A. Bradfield. The PAS superfamily: Sensors of environmental and developmental signals. Annu Rev. Pharmacol. Toxicol. 40, 519-561 https://doi.org/10.1146/annurev.pharmtox.40.1.519
  21. Halliwell, B., J. M. Gutteridge. 1985. The importance of free radicals and catalytic metal ions in human diseases. Mol. Aspects Med. 8, 89-193 https://doi.org/10.1016/0098-2997(85)90001-9
  22. Hansen, A. 1977. Extracellular potassium concentration in juvenile and adult brain cortex during anoxia. Acta. Physio.l Scand. 99, 412-420 https://doi.org/10.1111/j.1748-1716.1977.tb10394.x
  23. Hochachka, P.W., P. Lutz. 2001. Mechanism, origin, and evolution of anoxia tolerance in animals. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 130, 435-459 https://doi.org/10.1016/S1096-4959(01)00408-0
  24. Hsieh, M. T., W. H. Peng, C. R. Wu and W. H. Wang. 2000. The ameliorating effects of the cognitive-enhancing Chinese herbs on scopolamine-induced amnesia in rats. Phytother. Res. 14, 375-377 https://doi.org/10.1002/1099-1573(200008)14:5<375::AID-PTR593>3.0.CO;2-5
  25. Jeong, H. W., S. Y. Kang, S. W. Bak. 1999. Effect of Rhizoma Acori Graminei Extract on Blood Pressure and Regional Cerebral Blood Flow in Rats. The Korea Association of Herbology 14, 81-88
  26. Jin, W., L. F. Qu, P. Min, S. Chen, H. Li, H. Lu and Y. T. Hou. 2004. Identification of genes responsive to apoptosis in HL-60 cells. Acta Pharmacol Sin. 25, 319-326
  27. Kang, S. J., H. S. Keum, Y. Y. Jeon, E. J. Lee, C. S. Park, C. G. Park, J. H. Heo., C. H. Yang and J. S. Cho. 2001. The neuropropetective effect of Acori graminei rhizoma extract against cerebral ischemia in rats. Korean J. Orient.Int. Med. 22, 341-351
  28. Kerr, J. F. R., A. H. Wyllie and A. R. Currie. 1972. Apoptosis : a basic biological phenomenon with wideranging implications in tissue kinetics. Br. J. Cancer 26, 239-245 https://doi.org/10.1038/bjc.1972.33
  29. Kim, G. O., H. J. Kim, G. H. Park, G. H. Seok, S. Y. Kim, D. K. Ahn and H. C. Kim. 2000. Neuroprotective Effects of Acori graminei mizoma on the Brain Ischemia induced by Four - Vessel Occlusion in Rats. The Korea Association of Herbology 15, 79-85
  30. Kirino, T. 1982. Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res. 239, 57-69 https://doi.org/10.1016/0006-8993(82)90833-2
  31. Kirino, T. 1982. Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res. 239,57-69 https://doi.org/10.1016/0006-8993(82)90833-2
  32. Kirino, T., A. Tamura and K. Sano. 1985. Selective vulnerability of the hippocampus to ischemia-reversible and irreversible types of ischemic cell damage. Progress in Brain Res. 63, 39-58 https://doi.org/10.1016/S0079-6123(08)61974-3
  33. Kobori, N., G. L. Clifton and P. Dash. 2002. Altered expression of novel genes in the cerebral cortex following experimental brain injury. Brain Res. Mol. Brain Res. 104, 148-158 https://doi.org/10.1016/S0169-328X(02)00331-5
  34. Lee, U. J., G. N. Park S. G. Jeong, Y. Jun and S. G. Kim. 2003. Original Articles : Change in the Intrathecal Cytokine level in Hypoxic-ischemic Encephalopathy after Cardiac Arrest. The Korean society of Emergency Medicine 14, 494-498
  35. Levy, A.P., N. S. Levy, S. Wegner and M. A. Goldberg. 1995. Transcriptional regulation of the rat vascular endothelial growth factor gene by hypoxia. J. Biol Chem. 270, 13333-13340 https://doi.org/10.1074/jbc.270.22.13333
  36. Martin, L. J., A. M., Brambrink, C Lehmann, C Portera-Cailliau, R. C. Koehler, J. Rothstein and R. J. Traystman. 1997. Hypoxia-ischemia causes abnormalities in glutamate transporters and death of astroglia and neurons in newborn striatum. Ann Neurol. 42, 335-348 https://doi.org/10.1002/ana.410420310
  37. McDonald, W., F. S. Silverstein and M. V. Johnston. 1988. Neurotoxicity of N-methyl-D-aspartate is markedly enhanced in developing rat central nervous system. Brain Res. 459, 200-203 https://doi.org/10.1016/0006-8993(88)90306-X
  38. Nilsson, G. E. 2001. Surviving anoxia with the brain turned on. News Physiol Sci. 16, 217-221
  39. Nie, M., A. L. Blankenship and J. P. Giesy. 2001. Interactions between aryl hydrocarbon receptor(AhR) and hypoxia signaling pathways. Environ. Toxicol. Pharmacol. 10, 17-27 https://doi.org/10.1016/S1382-6689(01)00065-5
  40. Ramsay, G. 1998. DNA chips: state-of-the art. Nature Biotechnol. 16, 40-44 https://doi.org/10.1038/nbt0198-40
  41. Ribeiro, A., S. F. Bronk, P. J. Roberts, R. Urrutia and G. J. Gores. 1999. The ransforming growth factor beta(1)-inducible transcription factor TIEG1, mediates apoptosis through oxidative stress. Hepatology 30, 1490-1497 https://doi.org/10.1002/hep.510300620
  42. Rosenbaum, D. M., M. Michaelson, D. K. Batter, P. Doshi and J. A. Kessler. 1994. Evidence for hypoxia-induced, programmed cell death of cultured neurons. Ann Neurol. 36, 864-870 https://doi.org/10.1002/ana.410360610
  43. Schena, M., R. A. Heller, T. P. Theriault, K. Konrad, E. Lachenmeier and R. W. Davis. 1998. Microarrays: biotechnology's discovery platform for functional genomics. Trends Biotechnol. 16, 301-306 https://doi.org/10.1016/S0167-7799(98)01219-0
  44. Schwartzman, R. A., J. A. Cidlowski, 1993. Apoptosis: the biochemistry and molecular biology of programmed cell death. Endocrinol. 14, 133-151
  45. Semenza, G. L. 1998. Hypoxia-inducible factor 1: master regulator of $O_2$ homeostasis. Curr. Opin. Genet. Dev. 8, 588-594 https://doi.org/10.1016/S0959-437X(98)80016-6
  46. Semenza, G. L., P. H. Roth,H. M. Fang and G. L. Wang. 1994. Transcriptional regulation of genes encoding glycolytic enzymes by hypoxia-inducible factor 1. J. Biol Chem. 269, 23757-23763
  47. Semenza. G. L., B. H. Jiang, S. W. Leung, R. Passantino, J. P. Concordet, P. Maire and Giallongo A. 1996. Hypoxia response elements in the aldolase A, enolase 1, and lactate dehydrogenase A gene promoters contain essential binding sites for hypoxia-inducible factor 1. J. Biol Chem. 271, 32529-32537 https://doi.org/10.1074/jbc.271.51.32529
  48. Seo, G. S. 2003. Effects of Acori graminei rhizorna(石菖蒲) on the Modulation of ROS, MMP, in a Hypoxic Model of Cultured Rat Cortical Cells. Dongguk graduate school
  49. Watson, A., A. Mazumder, M. Stewart and S. Balasubramanian. 1998. Technology for microarray analysis of gene expression. Curr Opin Biotechnol. 9, 609-614 https://doi.org/10.1016/S0958-1669(98)80138-9
  50. Whitney, L.W., K. G. Becker, N. J. Tresser,C. I. Caballero-Ramos, P. J. Munsun, V. V. Prabhu, J. M. Trent, H. F. McFarland and W. E. Biddison. 1999. Analysis of gene expression in multiple sclerosis lesions using cDNA microarrays. Ann Neurol. 46, 425-428 https://doi.org/10.1002/1531-8249(199909)46:3<425::AID-ANA22>3.0.CO;2-O
  51. Widlak, P. 2004. DNA microarrays, a novel approach in studies of chromatin structure. Acta Biochim Pol. 51, 1-8
  52. Xu, Q., J. C. Reed. 1998. Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast. Mol. Cell. 1, 337-346 https://doi.org/10.1016/S1097-2765(00)80034-9
  53. Yukawa, K., K. Hoshino, M. Kishino, M. Mune, N. Shirasawa, A. Kimura, Y. Tsubota, K. Owada-Makabe, T. Tanaka, M. Ichinose, M. Maeda, K. Takeda and S. Akira. 2004. Deletion of the kinase domain in death-associated protein kinase attenuates renal tubular cell apoptosis in chronic obstructive uropathy. Int J. Mol Med. 13, 515-520
  54. Zimmermann, K. C., D. R. Green. 2001. How cells die: Apoptosis pathways. J. Allergy Clin Immunol. 108, 99-103 https://doi.org/10.1067/mai.2001.117819
  55. Son S. Y. 1979. sin-nong-bon-cho-gyung, pp. 38-39, Moonguangdoseoyuhangongsa, Taipei
  56. Lee S. J. 1999. Bon-cho-kang-mok, pp. 410-411, Science techonology book publisher. Baijing
  57. Wang-Ang. 1989. Bon-cho-bi-yo, pp. 44-45, Komoonsa. Seoul
  58. Hco. J. 1999. Dong-Eui-Bo-Gam, pp. 965, 961, 1955. Bubinbooks. Seoul

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