Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antimicrobial and Antioxidant Activities and Inhibition of Nitric Oxide Synthesis of Oak Wood Vinegar

참나무 목초액의 항균 및 항산화 활성과 일산화질소 합성 저해연구

  • Jung, Il-Sun (Department of Food and Nutrition, College of Medical Life Science Silla University) ;
  • Kim, Yu-Jung (Department of Food and Nutrition, College of Medical Life Science Silla University) ;
  • Gal, Sang-Wan (Department of Microbiological Engineering, Jinju National University) ;
  • Choi, Young-Ju (Department of Food and Nutrition, College of Medical Life Science Silla University)
  • 정일선 (신라대학교 의생명과학대학 식품영양학과) ;
  • 김유정 (신라대학교 의생명과학대학 식품영양학과) ;
  • 갈상완 (진주산업대학교 미생물공학과) ;
  • 최영주 (신라대학교 의생명과학대학 식품영양학과)
  • Published : 2007.01.29
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Abstract

This study was carried out to investigate the biological effects oak wood vinegar. Antimicrobial activity was tested in five microbial species at the concentration of 5 to $50{\mu}l$ of oak wood vinegar by paper disc method. Growth of P. oleovoranse, P. vulgaris, E. coli, S. aureus and Prevotella intermedia was inhibited at a dose of as low as $50{\mu}l$ of oak wood vinegar. Antioxidant activities were measured by using DPPH radical scavenging and SOD-like activity. DPPH radical scavenging and SOD-like activities were 90% and 65% at the concentration of $25{\mu}l\;and\;50{\mu}l$ of oak wood vinegar, respectively. Stimulation of the macrophages RAW264.7 cells with lipopolysaccharide (LPS) resulted in increased production of nitric oxide (NO) in the medium. However, the oak wood vinegar showed marked inhibition of NO synthesis in a dose-dependent manner. This result suggest that oak wood vinegar plays significant role for activation of immune system in the pathogenesis of inflammatory diseases.

참나무 목초액은 예로부터 농축산업에 이용되어 왔으며 최근에는 항균 및 항산화 효과가 있는 것으로 밝혀지면서 목초액에 대한 과학적인 연구가 활발히 진행되고 있다. 참나무 목초액의 항균 및 항산화 활성과 면역기능에 미치는 영향을 조사하였다. 참나무 목초액의 항균활성은 식품 부패균 및 치주질환 원인균에 대하여 항균활성을 조사하였는데 목초액 $50{\mu}l$ 첨가시 대부분의 식품부패균 및 치주질환 원인균의 생육이 현저히 저해되었다. 참나무 목초액의 항산화력은 DPPH법에서 $20{\mu}l$ 첨가시 95%의 라디칼 소거능을 보였으며 SOD유사활성은 $50{\mu}l$ 첨가시 65%의 항산화력을 나타내었다. 참나무 목초액의 항염증효과는 세균의 LPS를 처리하여 유도된 RAW264.7 세포에서 조사되었는데 LPS를 처리하여 유도된 NO 활성을 현저히 감소(86%)시켰으며 이 농도에서 세포독성 없이 높은 면역 효과를 나타내었다.

Keywords

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