Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of fisetin on UVB-induced apoptosis and DNA single strand breaks in NIH3T3 cells

NIH3T3 세포에서 UVB에 의한 세포고사와 DNA 단사절단에 미치는 fisetin의 효과

  • Jeong, Se-Jin (Institute of Basic Natural Science, Wonkwang University) ;
  • Kim, Don-Young (Division of Biological Science, Wonkwang University) ;
  • Han, Seol-Hee (Division of Biological Science, Wonkwang University) ;
  • Shin, Sang-Min (Division of Biological Science, Wonkwang University) ;
  • Cha, Jae-Young (Division of Biological Science, Wonkwang University) ;
  • Park, Nou-Bog (Department of Floriculture, Korea National Agricultural College) ;
  • Lee, Jung-Sup (Department of Biotechnology, Chosun University) ;
  • Park, Jong-Kun (Division of Biological Science, Wonkwang University)
  • 정세진 (원광대학교 기초과학연구소) ;
  • 김돈영 (원광대학교 생명과학부) ;
  • 한설희 (원광대학교 생명과학부) ;
  • 신상민 (원광대학교 생명과학부) ;
  • 차재영 (원광대학교 생명과학부) ;
  • 박노복 (한국 농업전문학교 화훼학과) ;
  • 이정섭 (조선대학교 생명공학과) ;
  • 박종군 (원광대학교 생명과학부)
  • Published : 2007.01.29
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Abstract

In the present study, we have investigated the effect of fisetin on the apoptosis and DNA single strand breaks in ultraviolet light B (UVB)-exposed NIH3T3 cells. Exposure of cells to UVB light $(200J/m^2)$ and post-incubation in growth medium for 48 hr resulted in about 50% of cells with apoptotic nuclear fragmentation. Addition of various concentrations of fisetin in the postincubation medium, however, significantly reduced the apoptotic nuclear fragmentation as compared with the values expected when the effects are additive and independent. DNA single strand breaks induced by UVB exposure were also significantly decreased by postincubation with fisetin. By Western blot analysis, fisetin post-incubation was shown to attenuate the p53 upregulation upon UVB exposure. Furthermore, the decrease of proliferating cell nuclear antigen (PCNA) level upon UVB exposure was alleviated by fisetin postincubation. These results suggest that fisetin decrease the apoptosis and increae DNA repair in a possible association with alteration of p53 and PCNA levels in UVB-exposed cells.

본 연구에서는 UVB에 조사된 NIH3T3 세포에서 세포고사와 DNA 단사절단에 미치는 fisetin후처리의 효과에 대해서 연구하였다. 세포에 UVB $(200J/m^2)$를 조사하고 정상배지에서 48시간 배양한 세포의 세포고사에 수반되는 핵분절은 50% 정도의 세포에서 관찰되었다. 흥미롭게도 배양배지에 fisetin이 첨가될 경우 핵분절을 보이는 세포의 빈도는 상당한 감소를 보였다. 알칼리 아가로스 겔에 의한 DNA 단사절단 분석에서 자외선 조사 후 fisetin처리는 정상배지 배양시보다 단사절단의 빈도를 감소시켜 DNA크기의 증가를 유도하였는데 이는 fisetin이 UVB에 의한 DNA 상해의 회복에 긍정적 효과를 나타냄을 시사한다 Western blot 분석에 의해 fisetin은 자외선 조사에 의해 활성화되는 p53의 수준을 유의한 수준으로 감소시키며 자외선 상해의 결과 세포주기의 정지에 수반되는 PCNA의 감소 경향을 다소 완화시켰다. 이러한 결과들은 fisetin이 DNA 회복의 활성을 통해 세포고사의 감소에 기여하며 이 과정에서 p53 및 PCNA의 수준변화와 관련하여 행동함을 시사한다.

Keywords

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