Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Optimal Culture Conditions for the Production of a Novel Extracellular Alkaline Lipase from Yarrowia lipolytica NRRL Y-2178

  • Lee, Geon-Ho (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Bae, Jae-Han (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Suh, Min-Jung (Department of Animal Science and Biotechnology, Kyungpook National University) ;
  • Kim, Hak-Ryul (Department of Animal Science and Biotechnology, Kyungpook National University)
  • Published : 2007.06.30
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Abstract

Lipases are industrially useful versatile enzymes that catalyze numerous different reactions. Among lipases functioning under extreme conditions, alkaline lipase is useful in detergent industry. Lipase from yeast strain Yarrowia lipolytica NRRL Y-2178 was most active under alkaline condition, and initial medium pH for most lipase production was also alkaline [Lee et al., 2007, J Microbiol Biotechnol, 17(6)]. High lipase production was achieved using Y. lipolytica NRRL Y-2178. Optimal incubation time for lipase production at $25^{\circ}C$ was 72 h. Optimal temperature, when incubated for 72 h, was $27.5^{\circ}C$. Lipase production but not cell growth was very sensitive to concentrations of glucose and glycerol as efficient carbon sources, showing optimal concentrations of 1.0 and 1.5% (w/v), respectively. Lipase production was highly stimulated by $Ca^{2+},\;K^+,\;and\;Na^+$, but was inhibited by $Co^{2+},\;Cu^{2+},\;Mn^{2+},\;Na^+,\;and\;Fe^{2+}$. Maximum lipase production at 0.1 mM $Ca^{2+}$ for 72 h incubation at $27.5^{\circ}C$ was 649 units/mL.

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References

  1. Aloulou A, Rodriguez JA, Puccinelli D, Mouz N, Leclaire J, Leblond Y, and Carriere F (2007) Purification and biochemical characterization of the LIP2 lipase from Yarrowia lipolytica. Biochim Biophys Acta Mol Cell Biol Lipids 1771, 228-237 https://doi.org/10.1016/j.bbalip.2006.12.006
  2. Castro-Ochoa LD, Rodriguez-Gomez C, Valerio-Alfaro G, and Ros RO (2005) Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. Enzyme Microb Technol 37, 648-654 https://doi.org/10.1016/j.enzmictec.2005.06.003
  3. Corzo G and Revah S (1999) Production and characteristics of the lipase from Yarrowia lipolytica 681. Bioresource Technol 70, 173-180 https://doi.org/10.1016/S0960-8524(99)00024-3
  4. Eckel RH (1989) Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases. N Engl J Med 320, 1060-1068 https://doi.org/10.1056/NEJM198904203201607
  5. Essamri M, Deyris V, and Comeau L (1998) Optimization of lipase production by Rhizopus oryzae and study on the stability of lipase activity in organic solvents. J Biotechnol 60, 97-103 https://doi.org/10.1016/S0168-1656(97)00193-4
  6. Fickers P, Ongena M, Destain J, Weekers F, and Thonart P (2006) Production and down-stream processing of an extracellular lipase from the yeast Yarrowia lipolytica. Enzyme Microb Technol 38, 756-759 https://doi.org/10.1016/j.enzmictec.2005.08.005
  7. Gulati R, Saxena RK, Gupta R, Yadav RP, and Davidson WS (1999) Parametric optimisation of Aspergillus terreus lipase production and its potential in ester synthesis. Process Biochem 35, 459-464 https://doi.org/10.1016/S0032-9592(99)00090-4
  8. Gulomova K, Ziomek E, Schrag JD, Davranov K, and Cygler M (1993) Purification and characterization of a Penicillium sp. Lipase which discriminates against diglycerides. Lipids 31, 379-384 https://doi.org/10.1007/BF02522923
  9. Gupta N, Sahai V, and Gupta R (2007) Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor. Process Biochem 42, 518-526 https://doi.org/10.1016/j.procbio.2006.10.006
  10. Harwood J (1989) The versatility of lipases for industrial uses. Trends Biochem Sci 14, 125-126 https://doi.org/10.1016/0968-0004(89)90140-0
  11. Hiol A, Jonzo MD, Druet D, and Comeau L (1999) Production, purification and characterization of an extracelluar lipase from Mucor hiemalis f. hiemalis. Enzyme Microb Technol 25, 80-87 https://doi.org/10.1016/S0141-0229(99)00009-5
  12. Hou CT (1997) Characterization of new yeast lipases. J Am Oil Chem Soc 74, 1391-1394 https://doi.org/10.1007/s11746-997-0242-6
  13. Kanwar L, Gogoi BK, and Goswami P (2002) Production of a Pseudomonas lipase in n-alkane substrate and its isolation using an improved ammonium sulfate precipitation technique. Bioresource Technol 84, 207-211 https://doi.org/10.1016/S0960-8524(02)00061-5
  14. Karadzic I, Masui A, Zivkovic LI, and Fujiwara N (2006) Purification and characterization of an alkaline lipase from Pseudomonas aeruginosa isolated from putrid mineral cutting oil as component of metalworking fluid. J Biosc Bioeng 102, 82-89 https://doi.org/10.1263/jbb.102.82
  15. Lee GH, Bae JH, Suh MJ, Kim IH, Hou CT, and Kim HR (2007) New finding and optimal production of a novel extracellular alkaline lipase from Yarrowia lipolytica NRRL Y-2178. J Microbiol Biotechnol (in press)
  16. Lin ES, Wang CC, and Sung SC (2006) Cultivating conditions influence lipase production by the edible Basidiomycete Antrodia cinnamomea in submerged culture. Enzyme Microb Technol 39, 98-102 https://doi.org/10.1016/j.enzmictec.2005.10.002
  17. Ratledge C and Tan KH (1990) Oils and fats: production degradation and utilization by yeasts. In Yeasts, Biotechnology and Biocatalysis, Verachter H and Mot D (eds.) pp 223-253. Marcel Dekker, New York
  18. Sarda L and Desnuelle P (1958) Action de la Lipase pancreatique Sur Les Esters en Emulsion. Biochim Biophys Acta 30, 513-521 https://doi.org/10.1016/0006-3002(58)90097-0
  19. Tan T, Zhang M, Wang B, Ying C, and Deng L (2003) Screening of high lipase producing Candida sp. and production of lipase by fermentation. Process Biochem 39, 459-465 https://doi.org/10.1016/S0032-9592(03)00091-8
  20. Vorderwiilbecke T, Kieslich K, and Erdmann H (1992) Comparison of lipases by different assays. Enzyme Microb Technol 14, 631-639 https://doi.org/10.1016/0141-0229(92)90038-P
  21. Yu M, Qin S, and Tan T (2007) Purification and characterization of the extracellular lipase Lip2 from Yarrowia lipolytica. Process Biochem 42, 384-391 https://doi.org/10.1016/j.procbio.2006.09.019