Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Comparison of Protein Binding Polysaccharide from Agaricus blazei Murill Prepared by Ultrafiltration and Spray-Drying Process

  • Hong, Joo-Heon (Bio Industry Center, Daegu New Technology Agency) ;
  • Choi, Yong-Hee (Department of Food Science and Technology, Kyungpook National University) ;
  • Youn, Kwang-Sup (Department of Food Science and Technology, Catholic University of Daegu)
  • Published : 2007.03.30
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Abstract

Chemical properties of spray-dried powders separated based on molecular weight from crude protein binding polysaccharide (CP-SD) of Agraricus blazei were examined. Contents of ${\beta}$-glucan in SD-1, SD-2 and SD-3 were 18.67%, 48.24%, and 37.15% respectively, and SD-2 (10-150 kDa) showed the highest molecular weight. Obtained ${\beta}$-glucans were not pure glucan, but was determined to be an acidic proteo-heteroglycan with a large amount of glucose (74.46-80.05%), galactose (8.91-15.2%), and mannose (4.9-5.46%). Composition of their amino acids was mainly aspartic and glutamic acids. FT-IR spectrum revealed SD-1, SD-2 and SD-3 were structures of ${\beta}$-1,3-glucans and ${\alpha}$-1,6-glucans at 890 and 930 $cm^{-1}$, respectively, signals of ${\alpha}$-1,6-glucans for CP-SD was not found. Useful CP-SD was recovered from A. blazei for preparation of three powder types as food materials.

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References

  1. Blumenkrantz N and Asboe-Hansen G (1973) New method for quantitative determination of uronic acids. Anal Biochem 54, 484-489 https://doi.org/10.1016/0003-2697(73)90377-1
  2. Chawla A, Taylor KMG, Newton JM and Johnson MCR (1994) Production of spray- dried salbutamol sulphate for use in dry powder aerosol formulations. Int J Pham 108, 233-240 https://doi.org/10.1016/0378-5173(94)90132-5
  3. Chen HK (1986) Studies on the characteristics of tasteactive components in mushroom concentrate and its powderization. MS Thesis, National Chung-Hsing University, Taichung, Taiwan
  4. Fujimiya Y, Suzuki Y, Oshima K, Kobori H and Moriguchi K (1998) Selective tumoricidal effect of soluble proteoglucan extracted from the basidiomycete, Agaricus blasei Murill, mediated via natural killer cell activation and apoptosis. Cancer Immunol Immun 46, 147-159 https://doi.org/10.1007/s002620050473
  5. Kacurakova M, Capek P, Sasinkova V, Wellner N and Ebringerova A (2000) FT-IR study of plant cell wall model compound: pectic polysaccharides and hemicelluloses. Carbohyd Poly 43, 195-203 https://doi.org/10.1016/S0144-8617(00)00151-X
  6. Kim GY, Park HS, Nam BH, Lee SJ and Lee JD (2003) Purification and characterization of acidic proteo-heteroglycan from the fruiting body of Phellinus linteus (Berk. & M.A. Curtis) Teng Biores Tech 89, 81-87 https://doi.org/10.1016/S0960-8524(02)00273-0
  7. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein measurement with the Folin-phenol reagents. J Biol Chem 193, 265-275
  8. McCleary BV and Holmes MG (1985) Enzymic quantification of (1-3),(1-4) $\beta$-glucan in barley and malt. J Institute Brewing 91, 285-295 https://doi.org/10.1002/j.2050-0416.1985.tb04345.x
  9. Mizuno T, Hagiwara T, Nakamura T, Ito H, Shimura K, Sumiya T and Asakura A (1990) Antitumor activity and some properties of water-soluble polysaccharides from 'Himematsutake', the fruiting body of Agaricus blazei Murill. Agric Biol Chem 54, 2889-2896 https://doi.org/10.1271/bbb1961.54.2889
  10. Nakajima A, Ishida T, Koga M and Takeuchi M (2002) Effect of hot water extract from Agaricus blasei Murill on antibody-producing cells in mice. Int Immunopharmacology 2, 1205-1211 https://doi.org/10.1016/S1567-5769(02)00056-5
  11. Ping H, Stanley S and Lisbeth I (1999) Chitosan microspheres prepared by spray- drying. Int J Pharmaceutics 187, 53-65 https://doi.org/10.1016/S0378-5173(99)00125-8
  12. Rey L, May J and Marcel D (2001) Freeze-drying/Lyophilization of Phamaceutical and Biological products. Eur J Pharma Biopharm 51, 163-164
  13. Saha SK and Brewer CF (1994) Determination of the concentrations of oligosaccharides, complex type carbohydrates, and glyco-proteins using the phenol-sulfuric acid method. Carbohyd Res 254, 157-167 https://doi.org/10.1016/0008-6215(94)84249-3
  14. Vlasta MG, Romano B and Gerwin JP (2001) Vibrational spectroscopic characterization of wild growing mushrooms and toadstools. Spetrochimi Acta A 57, 2815- 2829 https://doi.org/10.1016/S1386-1425(01)00584-4
  15. Yoshiyuki A, Naohito O, Masumi O, Shozo O and Toshiro Y (1990) Change of biological activities of (1-3)-$\beta$-Dglucan from Grifola frondosa upon molecular weight reduction by heat treatment. Chem Pharm Bull 38, 477- 481 https://doi.org/10.1248/cpb.38.477
  16. Zhbankov RG, Adrianov VM and Marchewka MK (1997) Fourier transform IR and Raman spectroscopy and structure of carbohydrates. J Mol Struct 436, 637-654 https://doi.org/10.1016/S0022-2860(97)00141-5