The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Selection and Identification of a Hyperparasite, Ampelomyces quisqualis 94013 for Biocontrol of Cucumber Powdery Mildew

오이 흰가루병 생물적 방제를 위한 중복기생균 Ampelomyces quisqualis 94013의 선발 및 동정

  • Lee, Sang-Yeob (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Hong, Sung-Kee (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kim, Yong-Ki (Plant Pathology Division, National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Kim, Hong-Gi (Department of Agricultural Biology, Chungnam National University)
  • 이상엽 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 홍성기 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 김용기 (농촌진흥청 농업과학기술원 식물병리과) ;
  • 김홍기 (충남대학교 농업생명과학대학 농생물학과)
  • Published : 2007.12.31
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Abstract

308 isolates of Ampelomyces sp. were isolated from powdery mildew fungi of 73 plant species in Korea for selection of biocontrol agents. An isolate 94013 isolated from powdery mildew fungus of red bean was selected as an effective biological control agent against cucumber powdery mildew in greenhouse. The morphological characteristics of the isolate is as follows. Pycnidia were sub-globose or elongated to pyriform, pale to dart brown, $52.5{\sim}82.5\;{\times}\;35.0{\sim}47.5\;(ave.\;62.5{\times}40.5){\mu}m$, and conidia were guttulate, straight cylindrical to fusiform, pale brown, $5.0{\sim}8.0\;{\times}\;2.5{\sim}4.3\;(ave.\;6.0{\times}3.0){\mu}m$. The isolate 94013 was identified as Ampelomyces quisqualis by morphological characteristics and rDNA ITS sequencing. The isolate A. quisqualis 94013 was different from that of the commercial product $AQ10^R$ in the rDNA ITS sequence.

국내에서 73종의 식물에서의 흰가루병균에서 중복기생균인 Ampelomyces 속 308 균주를 분리하였다. 팥의 흰가루병균에서 분리한 AQ94013 균주는 오이 흰가루병균에 가장 기생력이 우수한 균주로 선발되었다. 94013 균주의 병자각은 담갈색이나 다갈색이며, 구형이나 긴 서양배 모양으로, 그 크기는 $52.5{\sim}82.5{\times}35.0{\sim}47.5$(평균 $62.5{\times}40.5){\mu}m$이다. 병포자는 옅은 갈색의 단세포이며, 세포안에 유적이 보이고, 곧은 원통모양의 방추형이며, 크기는 $5.0{\sim}8.0{\times}2.5{\sim}4.3$ (평균 $6.0{\times}3.0){\mu}m$이다. 그러므로 AQ94013 균주는 형태학적 및 분자적 특성을 조사한 결과에서 Ampelomyces quisqualis로 동정하였다. 또한 Ampelomyces quisqualis 94013균주는 기 상업화한 Ampelomyces sp. AQ10균주 등의 rDNA ITS sequence를 비교 분석한 결과에서 다른 균주임이 증명되었다.

Keywords

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