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유산균을 함유한 알긴산 미세입자의 제조와 특성

Preparation and Characterization of Lactic Acid Bacteria Encapsuled with Alginate Microsphere

  • 최창용 (순천대학교 고분자공학과) ;
  • 강성구 (순천대학교 공동실험실습관) ;
  • 박석규 (순천대학교 식품영양학과) ;
  • 장미경 (순천대학교 고분자공학과) ;
  • 나재운 (순천대학교 고분자공학과)
  • Choi, Chang-Yong (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Kang, Seong-Koo (Research Instrument Center, Sunchon National University) ;
  • Park, Seok-Kyu (Department of Food and Nutrition, Sunchon National University) ;
  • Jang, Mi-Kyeong (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Nah, Jae-Woon (Department of Polymer Science and Engineering, Sunchon National University)
  • 발행 : 2007.12.30

초록

해조류에서 추출한 다양한 생리활성을 갖고 있는 alginate를 유산균 담지체로 하여 유산균을 담지한 결과, 아주 작은 구형의 유산균 미세입자를 제조할 수 있었다. 유산균 미세입자는 위에서는 전혀 분해가 일어나지 않은 상태에서 유산균을 안정하게 담지하고 있다가 대장에서 분해가 됨으로써 유산균의 활성을 나타내는 것을 알 수 있었다. 제조된 유산균 미세입자는 위에서 유산균의 사멸을 방지하므로서 효과적으로 대장까지 전달시킬 수 있을 것이다. 특히 유산균 미세입자는 유산균의 자체효과 외에 유산균을 담지하기 사용된 alginate와 코팅제로 사용된 키토산의 유용한 생리 활성을 모두 나타낼 수 있는 다기능성 유산균 미세입자로 응용이 가능할 것으로 판단된다.

This study is performed to assess the preparation and characterization of lactic acid bacteria (Sterpoccoccus thermophilus) loaded with alginate microsphere using alginate and chitosan for the efficient delivery of lactic acid bacteria to large intestine. Size and morphology of alginate microsphere were confirmed $6{\sim}10{\mu}m$ with spherical shape by scanning electronic microscope (SEM). Biodegradation study of alginate was investigated at different buffer solutions (pH 1.2 and 7.4). This result showed that alginate microsphere did not degrade at pH 1.2 buffer solution but it's degradation occurred from first day at pH 7.4 buffer solution. Survivability test of lactic acid bacteria in alginate microsphere showed that it was keeping activity of lactic acid bacteria by chroma meter. Therefore, the introduction of alginate microsphere might be a potential system to efficiently delivery lactic acid to large intestine.

키워드

참고문헌

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