Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Preparation and Characterization of Lactic Acid Bacteria Encapsuled with Alginate Microsphere

유산균을 함유한 알긴산 미세입자의 제조와 특성

  • Choi, Chang-Yong (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Kang, Seong-Koo (Research Instrument Center, Sunchon National University) ;
  • Park, Seok-Kyu (Department of Food and Nutrition, Sunchon National University) ;
  • Jang, Mi-Kyeong (Department of Polymer Science and Engineering, Sunchon National University) ;
  • Nah, Jae-Woon (Department of Polymer Science and Engineering, Sunchon National University)
  • 최창용 (순천대학교 고분자공학과) ;
  • 강성구 (순천대학교 공동실험실습관) ;
  • 박석규 (순천대학교 식품영양학과) ;
  • 장미경 (순천대학교 고분자공학과) ;
  • 나재운 (순천대학교 고분자공학과)
  • Published : 2007.12.30
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Abstract

This study is performed to assess the preparation and characterization of lactic acid bacteria (Sterpoccoccus thermophilus) loaded with alginate microsphere using alginate and chitosan for the efficient delivery of lactic acid bacteria to large intestine. Size and morphology of alginate microsphere were confirmed $6{\sim}10{\mu}m$ with spherical shape by scanning electronic microscope (SEM). Biodegradation study of alginate was investigated at different buffer solutions (pH 1.2 and 7.4). This result showed that alginate microsphere did not degrade at pH 1.2 buffer solution but it's degradation occurred from first day at pH 7.4 buffer solution. Survivability test of lactic acid bacteria in alginate microsphere showed that it was keeping activity of lactic acid bacteria by chroma meter. Therefore, the introduction of alginate microsphere might be a potential system to efficiently delivery lactic acid to large intestine.

해조류에서 추출한 다양한 생리활성을 갖고 있는 alginate를 유산균 담지체로 하여 유산균을 담지한 결과, 아주 작은 구형의 유산균 미세입자를 제조할 수 있었다. 유산균 미세입자는 위에서는 전혀 분해가 일어나지 않은 상태에서 유산균을 안정하게 담지하고 있다가 대장에서 분해가 됨으로써 유산균의 활성을 나타내는 것을 알 수 있었다. 제조된 유산균 미세입자는 위에서 유산균의 사멸을 방지하므로서 효과적으로 대장까지 전달시킬 수 있을 것이다. 특히 유산균 미세입자는 유산균의 자체효과 외에 유산균을 담지하기 사용된 alginate와 코팅제로 사용된 키토산의 유용한 생리 활성을 모두 나타낼 수 있는 다기능성 유산균 미세입자로 응용이 가능할 것으로 판단된다.

Keywords

References

  1. Armstrong, B., A. J. Van Merwyk and H. Coates. 1977. Blood pressure in seventh-day advantist vegeterians. Am. J. Epidemiol. 105, 444-449 https://doi.org/10.1093/oxfordjournals.aje.a112403
  2. Brine, C. J., P. A. Sandford and J. P. Zikakis. 1992. Advances in Chitin and Chitosan. pp. 659-670. Elsevier Applied Science, London
  3. Cosby, N. C. 1950. Microencapsulation of single, multiple, zona pellusida-free mouse preimplatation embryos in sodium alginate and their development in vitro. J. Repro. Fert. 90, 19-57
  4. Deeth, H. C. 1984. Yoghurt and culture's products. Aus. J. Dairy Technol. 39, 111-113
  5. Fujihara, M. and T. Nagumo. 1993. An influence of the structure of alginate on the chemotectic activity of macrophages and the antitumor activity. Carbohyadrate Res. 243, 211-245 https://doi.org/10.1016/0008-6215(93)84094-M
  6. Fujike, K., H. Matsuyama and T. Yano. 1994. Protective effect of sodium alginates against bacterial infection in common carp, Cyprinus carpio L. J. Fish Dis. 17, 349-354 https://doi.org/10.1111/j.1365-2761.1994.tb00230.x
  7. Go, K. M., J. S. Koo, Y. I. Kim and J. H. Yang. 1999, Preparation and stability of sodium alginate beads containing $\beta$-carotene. J. Kor. Pharm. Sci. 29, 323-327
  8. Guerrero, L., F. Omil, R. Mendez and J. M. Lema. 1998. Protein recovery during the overall treatment of waste waters from fish-meal factories. Bioresource Technology 63, 221-228 https://doi.org/10.1016/S0960-8524(97)00140-5
  9. Gebelein, C. G. and R. L. Dunn. 1990. Progress in Biomedical Polymers. pp. 283-290. Plenum Press, New York
  10. Haug, A., B. Larsen and O. Smidsrod. 1967. Studies on the sequence of the uronic acid residue in alginic acid. Acta. Chem. Scand. 21, 691-704 https://doi.org/10.3891/acta.chem.scand.21-0691
  11. Haug, A. and O. Smidsrod. 1965. The effect of divalent metals on the properties of alginate. Acta. Chem. Scand. 19, 341-347 https://doi.org/10.3891/acta.chem.scand.19-0341
  12. Han, S. C., E. J. Heo, K. Y. Lee, Y. Z. Kim and J. C. Kim. 2003. Antioxidant effect of vitamin-C/alginate gel-entrapped liposomes for resistance of DHA autoxidation. Korean J. Biotechnol. Bioeng. 18, 229-233
  13. Ilum, L. 1998. Chitosan and its use as a pharmaceutical excipient. Pharmaceutical Research 15, 1326-1332 https://doi.org/10.1023/A:1011929016601
  14. Jung, J. Y., S. S. Hui and Y. H. Choi. 1999. Studies on the efficient extraction process of alginic acid in sea tangle. Food Engineering Progress 3, 90-97
  15. Kim, K., K. I. Jang, C. H. Kim and K. Y. Kim. 2002. Optimization of culture conditions and encapsulation of Lactobacillus fermentum YL-3 for probiotics. Korean J. Food Sci. Technol. 34, 255-262
  16. Kimmura, T., K. Takahashi, Y. Ueda, H. Obika, Y. Kobayashi and K. Tsuji. 1993. Effects of the primary structure of alginate on fecal excretion of sodium in rats. Nippon Nogeikagaku Kaishi 67, 1177-1183 https://doi.org/10.1271/nogeikagaku1924.67.1177
  17. Kobayashi, N., Y. Kanazawa, S. Yamabe, K. Iwata, M. Nishizawa, T. Yamagishi, O. Nishikaze and K. Tsuji. 1997. Effects of depolymerized sodium alginate on serum total cholesterol in healthy women with a high cholesterol intake. J. Home Ecom. Japan 48, 255-230
  18. Lee, K. W., K. I. Jang, Y. B. Lee, J. S. Sohn and K. Y. Kim. 2007. Development of probiotic microcapsules for the preservation of cell viability. Korean J. Food Sci. Technol. 39, 66-70
  19. Lim, Y. S. and B. J. You. 2005. Effects of dialysis and various drying methods on physical properties of alginates prepared from sea tangle, Laminaria japonica. J. Kor. Fish. Soc. 38, 226-231 https://doi.org/10.5657/kfas.2005.38.4.226
  20. Lee, H. J., H. S. Kim, K. H. Chung, E. S. Lee and U. H. Chun. 1999. Optimization of the condition of immobilized Photobacterium phosphoreum with strontium alginate. Kor. J. Appl. Microbiol. Biotechnol. 27, 136-144
  21. Lee, K. Y. and T. R. Heo. 1998. Particle size effects in buffer system using calcium carbonate bead immobilized with alginate for the cultivation of Bifidobacterium. Korean J. Food Sci. Technol. 30, 425-433
  22. Lee, S. B., S. M. Seo, Y. M. Lim, S. K. Cho and Y. M. Lee. 2004. Preparation of alginate/poly(N-isopropylacrylamide) hydrogels using gamma-ray irradiation grafting. Maromolecular Res. 12, 269-275
  23. Modrzejewska, Z. and W. Kaminski. 1999. Separation of Cr(VI) on chitosan membranes. Industrial Engineering Chemistry Res. 38, 4946-4950 https://doi.org/10.1021/ie980612g
  24. Morichi., T. 1974. Preservation of lactic acid bacteria by freeze-drying. Jpn. Agric. Rec. Quart. 8, 171-176
  25. Olivia, F., B. Pierre and G. Robert. 1998. Chitosan: A unique polysaccharide for drug delivery. Drug Development Industrial Pharmacy 24, 979-985 https://doi.org/10.3109/03639049809089942
  26. Park, B. G., J. H. Lee, H. K. Shin, J. H. Lee and P. S. Chang. 2006. Optimization of conditions for the double layer microencapsulation of lactic acid bacteria. Korean J. Food Sci. Technol. 38, 767-772
  27. Rege, P. R., D. J. Shukla and L. H. Block. 1999. Chitosans as tableting excipients for modified release delivery systems. International J. Pharmaceutics 181, 49-60 https://doi.org/10.1016/S0378-5173(98)00416-5
  28. Son, E. H., Y. S. Yoon and S. Pyo. 1999. Immunomodulating activity of alginate. J. Applied Pharmacolcogy 7, 377-384
  29. Singh, D. K. and A. R. Ray. 1999. Controlled release of glucose through modified chitosan membranes. J. Membrane Science 155, 107-122 https://doi.org/10.1016/S0376-7388(98)00311-1
  30. Yang, J. H. and J. P Lim. 1998. Antibacterial effect of calcium alginate microcapsule containing chitosan. J. Kor. Pharm. Sci. 28, 151-158

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