Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Single Total Body Irradiation (TBI) on the Peripheral Blood of Piglets

돼지에서 단일 전신 방사선 조사 용량에 따른 혈액변화 관찰

  • Lee, Jae-Hoon (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Kang, Eun-Hee (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Chung, Dai-Jung (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Dong-Ku (College of Medicine, CHA Stem Cell Institute Pochon CHA University) ;
  • Kim, Jae-Hwan (Cell and Gene Therapy Research Institute, CHA Stem Cell Institute, Pochon CHA University) ;
  • Shin, Hyun-Soo (Department of Radiation Oncology, Bundang CHA Hospital, Pochon CHA University) ;
  • Ahn, Jung-Yong (Department of Neurosurgery, Yonsei Brain Research Institute, Yonsei University College of Medicine) ;
  • Park, Jin-Ki (Animal Biotechnology Division, National Institute of Animal Science) ;
  • Chang, Won-Kyong (Animal Biotechnology Division, National Institute of Animal Science) ;
  • Choi, Chi-Bong (Department of Biomedical Engineering, College of Medicine, The Catholic University of Korea) ;
  • Kim, Hwi-Yool (Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University)
  • Published : 2007.12.30
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Abstract

We examined total body irradiation (TBI)-induced effects by complete blood count (CBC) and fluorescence-activated cell sorter analysis (FACS) in the piglet following radiation irritation. A CBC included red blood cell count, white blood cell count, and platelet cell count. Four piglets were examined in this study and each piglet was divided by irradiation dose, two piglets with 4 Gy, two with 6 Gy, one with 8 Gy. All piglets showed leukopenia, thrombocytopenia after irradiation. In 6 and 8 Gy group, three piglets showed severe hemostatic disorder and gastrointestinal disorder suchas diarrhea and anorexia, and they died between 10 and 15 days after radiation irritation. In 4 Gy, two piglets showed no clinical sign after radiation injury, but persistent leukopenia was shown in blood examination. We suggest that a single TBI dose less than 6 Gy is adequate for conditioning piglet for bone marrow transplantation.

4주령의 돼지에서 여러 용량의 방사선 조사를 하고 경시적으로 혈액 수치의 변화, helper T-cell 및 cytotoxic T-cell에 대한 변화를 관찰하였다. 6 Gy 이상을 조사한 돼지에 3일 이후 백혈구와 혈소판의 수의 감소되어 회복되지 않았으며, 식욕 부진,설사 등의 부작용을 보이며, 조사 후 10일부터 15일 사이에 모두 폐사 하였다. 4 Gy 전신 단일 조사한 돼지에서는 조사 후 임상적인 부작용을 보이지 않았으며, 9일 이후 백혈구, 혈소판이 증가하여 회복되는 경향을 보였다. 본 연구의 결과는 돼지를 사용한 방사선 생물학적 실험에서 혈액변화 및 면역억압 관찰에 대한 기초 자료로 활용 될 수 있을 것이다.

Keywords

References

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