생명과학회지 (Journal of Life Science)

  • 제17권12호
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  • Pages.1622-1626
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  • 2007
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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DOI QR Code

버크셔 품종의 돼지 성장과 육질관련 후보유전자의 단일염기 다형성에 관한 연구

Investigation of Single Nucleotide Polymorphisms in Porcine Candidate Gene for Growth and Meat Quality Traits in the Berkshire Breed

  • 김상욱 (부산대학교 생명자원과학대학 생명자원과학부) ;
  • 정지혜 (부산대학교 생명자원과학대학 생명자원과학부) ;
  • 도경탁 (충북대학교 축산학과) ;
  • 김관석 (충북대학교 축산학과) ;
  • 도창희 (충남대학교 농업생명대학 동물자원학부) ;
  • 박준규 (경상남도 축산진흥연구소) ;
  • 주영국 (경상남도 축산진흥연구소) ;
  • 김태숙 (경상남도 축산진흥연구소) ;
  • 최봉환 (농촌진흥청 축산연구소) ;
  • 김태헌 (농촌진흥청 국제기술협력과) ;
  • 송기덕 (미국국립보건원) ;
  • 조병욱 (부산대학교 생명자원과학대학 생명자원과학부)
  • Kim, Sang-Wook (Department of Animal Science, Pusan National University) ;
  • Jung, Ji-Hye (Department of Animal Science, Pusan National University) ;
  • Do, Kyung-Tag (Department of Animal Science, Chungbuk National University) ;
  • Kim, Kwan-Suk (Department of Animal Science, Chungbuk National University) ;
  • Do, Chang-Hee (Department of Animal Science, Chungnam National University) ;
  • Park, Jun-Kyu (Livestock Veterinary Research Institute) ;
  • Joo, Young-Kuk (Livestock Veterinary Research Institute) ;
  • Kim, Tae-Suk (Livestock Veterinary Research Institute) ;
  • Choi, Bong-Hwan (Animal Genomics and Bioinformatics Division, National Livestock Research Institute) ;
  • Kim, Tae-Hun (International Technical Cooperation Center, Rural Development Administration) ;
  • Song, Ki-Duk (NICHD/National Institutes of Health) ;
  • Cho, Byung-Wook (Department of Animal Science, Pusan National University)
  • 발행 : 2007.12.30
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초록

4개의 후보 유전자를 분석해본 결과, 돼지의 주요 염색체 부위 및 유전자들이 주요 경제성 요인들과 관계가 있는 것으로 확인됐다. 양돈업계에서 DNA 기술을 이용한 염색체 정보를 활용하기 위해 본 연구에서는 4개의 후보 유전자에서 생성된 중합효소연쇄반응(PCR) 생성물을 비교 재 서열 함으로써 단일염기변이(SNP) 표지들을 개발했다. 또한 이들 4개의 SNP에 대해 PCR 제한효소 절편길이 다형 성(RFLP)분석을 전개한 후, 이를 대한민국 내 버크셔 종 돼지 개체군의 유전자형을 분석하는데 활용했다. 본 연구는 유용한 단일염기변이를 식별하고 돼지개체군 내 경제적으로 중요한 특성들과 SNP의 연관성을 확인하는 데 그 목적이 있다.

This study was conducted to identify useful single nucleotide polymorphisms (SNPs) and determine their association with economically important traits in pig population. Four candidate gene analyses have identified important chromosomal regions and major genes associcated whit economic traits of the pig. For application of the chromosomal information to the pig industry using DNA technology, SNP markers were developed by comparative re-sequencing of polymerase chain reaction (PCR) products of 4 candidate genes (CSF2, IL4, MYOD, RIP140). PCR restriction fragment length polymorphism (PCR-RFLP) assays were developed for these 4 SNPs and used to genotype Berkshire pig populations in Korea.

키워드

참고문헌

  1. Fahrenkrug, S. C., T. P. L. Smith, G. A. Rohrer and J. W. Keele. 2002. Single nucleotide polymorphism (SNP) discovery in porcine expressed genes. Animal Genetics 33, 186-195 https://doi.org/10.1046/j.1365-2052.2002.00846.x
  2. Fujii, J., K. Otsu, F. Zorzato, L. S. de, V. K. Khanna, J. E. Weiler, P. J. O'Brien and D. H. MacLennan. 1991. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science 253, 448-451 https://doi.org/10.1126/science.1862346
  3. Jungerius, B. J., A. P. Rattink, R. P. Crooijmans, J. J. Poel, B. A. Oost, M. F. Pas and M. A. Groenen. 2003. Development of a single nucleotide polymorphism map of porcine chromosome 2. Anim. Genet. 34, 429-437 https://doi.org/10.1046/j.0268-9146.2003.01056.x
  4. Kim, J. J., M. F. Rothschild, J. Beever, S. Rodriguez-Zas and J. C. Dekkers. 2005. Joint analysis of two breed cross populations in pigs to improve detection and characterization of quantitative trait loci. J. Anim. Sci. 83, 1229-1240 https://doi.org/10.2527/2005.8361229x
  5. Kim, T. H., K. S. Kim, B. H. Choi, D. H. Yoon, G. W. Jang, K. T. Lee, H. Y. Chung, H. Y. Lee, H. S. Park and J. W. Lee. 2005. Genetic structure of pig breeds from Korea and China using microsatellite loci analysis. J. Anim. Sci. 83, 2255-2263 https://doi.org/10.2527/2005.83102255x
  6. Kollers, S., K. Megy and D. Rocha. 2005. Analysis of public single nucleotide polymorphisms in commercial pig populations. Animal Genetics 36, 426-431 https://doi.org/10.1111/j.1365-2052.2005.01329.x
  7. Le, R. P., J. Naveau, J. M. Elsen and P. Sellier. 1990. Evidence for a new major gene influencing meat quality in pigs. Genet. Res. 55, 33-40 https://doi.org/10.1017/S0016672300025179
  8. Malek, M., J. C. Dekkers, H. K. Lee, T. J. Baas, K. Prusa, E. Huff-Lonergan and M. F. Rothschild. 2001, A molecular genome scan analysis to identify chromosomal regions influencing economic traits in the pig. II. Meat and muscle composition. Mamm. Genome 12, 637-645 https://doi.org/10.1007/s003350020019
  9. Meyers, S. N., M. B. Rogatcheva, D. M. Larkin, M. Yerle, D. Milan, R. J. Hawken, L. B. Schook and J. E. Beever. 2005. Piggy-BACing the human genome II. A high-resolution, physically anchored, comparative map of the porcine autosomes. Genomics 86, 739-752 https://doi.org/10.1016/j.ygeno.2005.04.010
  10. Park, K. G., K. S. Park, M. J. Kim, H. S. Kim, Y. S. Suh, J. D. Ahn, K. K. Park, Y. C. Chang and I. K. Lee. 2004. Relationship between serum adiponectin and leptin concentrations and body fat distribution. Diabetes Res. Clin. Pract. 63, 135-142 https://doi.org/10.1016/j.diabres.2003.09.010
  11. Van, D. S. H., G. F. W. Prall and G. S. Plastow. 2005. Application of genomics in the pork industry. Journal of Animal Science 83, E1-E8 https://doi.org/10.2527/2005.8311
  12. Van, W. H. J., B. Dibbits, E. E. Baron, A. D. Brings, B. Harlizius, M. A. Groenen, E. F. Knol and H. Bovenhuis. 2006. Identification of quantitative trait loci for carcass composition and pork quality traits in a commercial finishing cross. J. Anim. Sci. 84, 789-799 https://doi.org/10.2527/2006.844789x
  13. Wimmers, K., I. Fiedler, T. Hardge, E. Murani, K. Schellander and S. Ponsuksili. 2006. QTL for microstructural and biophysical muscle properties and body composition in pigs. BMC Genet. 9, 7-15

피인용 문헌

  1. Comparative transcriptomic analysis by RNA-seq to discern differential expression of genes in liver and muscle tissues of adult Berkshire and Jeju Native Pig vol.546, pp.2, 2014, https://doi.org/10.1016/j.gene.2014.06.005
  2. Comparative transcriptomic analysis to identify differentially expressed genes in fat tissue of adult Berkshire and Jeju Native Pig using RNA-seq vol.41, pp.9, 2014, https://doi.org/10.1007/s11033-014-3513-y