Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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The Chemical Constituents of the Stem Barks of Fraxinus rhynchophylla

물푸레나무(Fraxinus rhynchophylla) 수피의 추출성분

  • Yang, Eun-Ju (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Dong-Geun (Agrobiotechnology Education Center, Kyungpook National University) ;
  • Lee, Jong-Won (Catholic University of Daegu) ;
  • Kim, Yae-Sil (Hypoxy Co., Ltd., Catholic University of Daegu) ;
  • Lim, Sun-Ha (Hypoxy Co., Ltd., Catholic University of Daegu) ;
  • Song, Kyung-Sik (School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University)
  • 양은주 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 이동근 (경북대학교 생물건강.농업생명 인재양성사업단) ;
  • 이종원 (대구가톨릭대학교 의과대학) ;
  • 김예실 ((주)하이폭시) ;
  • 임선하 ((주)하이폭시) ;
  • 송경식 (경북대학교 농업생명과학대학 응용생명과학부)
  • Published : 2007.12.31
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Abstract

The stem barks of Fraxinus rhynchophylla was extracted with 95% EtOH, and the concentrated extract was successively partitioned with $CH_2Cl_2$, n-BuOH, and $H_2O$ in order to investigate the major phytochemicals. From the $CH_2Cl_2$ soluble fraction, a sterol (1) was isolated through the repeated silica gel column chromatographies. Three additional compounds (2-4) were isolated from the n-BuOH soluble fraction through silica gel, RP-18, and Sephadex LH-20 column chromatographies. Their chemical structures were elucidated as daucosterol $(1;{\beta}-sitosterol-3-O-{\beta}-D-glucopyranoside)$, caffeic acid (2), 6,8-dihydroxy-7-methoxycoumarin (3), and coniferaldehyde glucoside (4) by comparing their spectral data with those in the literatures. All isolates (1-4) were the first to be isolated from F. rhynchophylla.

물푸레나무(F. rhynchophylla)의 수피를 95% EtOH로 추출하여 얻어진 추출물을 $CH_2Cl_2$, n-BuOH 및 $H_2O$ 순으로 분획하였다. 이 중 $CH_2Cl_2$, 가용성 분획에 대하여 silica gel column chromatography를 실시하여 1종의 sterol 화합물(1)을 분리하였다. 한편 n-BuOH 가용성 분획에 대해서도 silica gel, RP-18 및 Sephadex LH-20 등의 충진제를 사용하여 column chromatography를 행하여 3종의 phenylpropanoid 화합물(2-4)을 얻었다. 각 화합물의 구조는 $^1H-NMR$와 LC-MS data를 해석하고 문헌과 비교하여 daucosterol(1), caffeic acid(2), 6,8-dihydroxy-7-methoxycoumarin(3) 및 coniferaldehyde glucoside(4)로 동정하였다. 이들 화합물은 진피에서 처음으로 분리되었다.

Keywords

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