Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Evaluation of Selective Media for Isolation of Foodborne Bacteria

주요 식중독균 분리용 선택배지의 타당성 연구

  • Jo, Seo-Hee (Department of Food Science and Technology, Chung-Ang University) ;
  • Ha, Ji-Hyoung (Department of Food Science and Technology, Chung-Ang University) ;
  • Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University) ;
  • Shim, Young-Hwan (Department of Food Science and Biotechnology and Institute of Bioscience and Biotechnology, Kangwon National University) ;
  • Kwon, Ki-Sung (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Han, Jeong-A (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Hwang, In-Gyun (Food Microbiology Division, Korea Food & Drug Administration) ;
  • Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University) ;
  • Oh, Deog-Hwan (Department of Food Science and Biotechnology and Institute of Bioscience and Biotechnology, Kangwon National University)
  • 조서희 (중앙대학교 식품공학과) ;
  • 하지형 (중앙대학교 식품공학과) ;
  • 김근성 (중앙대학교 식품공학과) ;
  • 심영환 (강원대학교 식품생명공학과) ;
  • 권기성 (식품의약품안전청 식품미생물팀) ;
  • 한정아 (식품의약품안전청 식품미생물팀) ;
  • 황인균 (식품의약품안전청 식품미생물팀) ;
  • 하상도 (중앙대학교 식품공학과) ;
  • 오덕환 (강원대학교 식품생명공학과)
  • Published : 2007.12.31
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Abstract

This study was conducted to evaluate the selective media listed in currently available Food Code in Korea. The 29 different types of media of five different types of foodborne bacteria including Salmonella spp., Bacillus cereus, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus were tested in the broth and food. The recovery test for five different types of foodborne bacteria was performed in the artificially inoculated into chicken, rice, pork and mackerel. There was no significant differences in isolation capabilities among twenty nine different types of isolation selective media for five different types of foodborne bacteria in broth condition, while there was significantly a little differences in isolation capabilities among those on foods (P<0.05). The higher number of foodborne pathogens were isolated from conventional selective media approved in Food Code than newly developed selective media such as chromogenic media. This results suggest that there was differences of selectivities among currently available isolation selective media in many countries and further studies are needed to be approved by Korean Food and Drug Administration.

본 연구는 현행 식품공전상 제시되어 있는 식중독균 분리배지의 평가를 위해 Salmonella spp., Bacillus cereus, Listeria monocytogenes, Vibrio parahaemolyticus, Staphylococcus aureus의 5가지 식중독세균용 29가지 분리 선택배지를 Broth와 Food에서 시험하였다. Food는 닭고기, 쌀, 돼지고기, 고등어를 대상으로 5가지 식중독균을 접종후 균주의 recovery를 확인하였고. Broth는 5가지 식중독세균용 29가지 분리 선택배지를 사용하였다. Broth에서는 각 세정균에 대한 분리배지들 간에 유의적인 차이가 없었으나(P<0.05), Food에서는 그 차가 크지는 않았으나, 분리선택배지간의 분리능에 통계적 유의차가 존재하였다. 식품공전에 등재된 전통적인 분리 선택배지에서 분리된 균수가 Chromogenic 배지 등 새로운 선택배지에서 분리된 균수보다 많았다. 국제적으로 등재된 배지간의 선택성의 차이가 존재하였으며, 추후 다양한 배지가 식품공전에 등재되기 위해서는 추가 연구가 필요하다.

Keywords

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