Analysis of Benzene in Beverages by Headspace-GC/MS

Headspace-GC/MS를 이용한 음료 중 벤젠 분석

  • Kim, Eun-Ju (New Hazard Chemicals Team, Korea Food & Drug Administration) ;
  • Park, Sang-Aeh (New Hazard Chemicals Team, Korea Food & Drug Administration) ;
  • Choi, Dong-Mi (New Hazard Chemicals Team, Korea Food & Drug Administration)
  • 김은주 (식품의약품안전청 신종유해물질팀) ;
  • 박상애 (식품의약품안전청 신종유해물질팀) ;
  • 최동미 (식품의약품안전청 신종유해물질팀)
  • Published : 2007.12.31

Abstract

A selective analytical method of headspace-GC/MS has been applied to determine levels of benzene in beverages. Food samples were 85 including 2 fruit juices, 6 fruit beverages, 11 carbonated beverages, 55 mixed beverages, and 4 beverage concentrations, and 7 extracted beverages. For phase equilibration of headspace, sample was stirred at $40^{\circ}C$ for 30 min. The oven temperature was $60^{\circ}C$ and elevated to $180^{\circ}C$ at $15^{\circ}C/min$. The internal standard was benzene-d6. The identification was done by the selected target ions such as m/z 51, 77, and 78 and the confirmation was done by the response ratio of m/z 77 to m/z 78 between sample and standard. The overall recoveries were ranged from 91% to 101% and the limit of quantification was $1{\mu}g/kg$. The average level of benzene were $5{\mu}g/kg$ for fruit beverages, $2{\mu}g/kg$ for carbonated beverages, $7{\mu}g/kg$ for mixed beverages and $7{\mu}g/kg$ for extracted beverages.

헤드스페이스-기체크로마토그래프/질량분석기를 이용하여 음료류 중 벤젠을 분석하였다. 대상식품은 시중에 유통되는 과실쥬스 2종, 과실음료 6종, 착향탄산음료 11종, 혼합음료 55종, 추출음료 7종, 음료베이스 4종 등 총 85종이었다. 헤드스페이스의 상평형을 위하여 $40^{\circ}C$에서 30분간 교반하였으며 오븐온도는 초기 온도 $60^{\circ}C$에서 5분간 유지 후 $15^{\circ}C$/분의 비율로 $180^{\circ}C$까지 상승하였다. 내부표준물질로는 벤젠-d6을 사용하였다. 선택이온 m/z 51, 77, 78로 확인하였으며 선택이온 m/z 78에 대한 m/z 77의 반응세기비로 재검증하였다. 벤젠의 선택이온 m/z 78과 내부표준물질 벤젠-d6의 선택이온 m/z 84에 대한 피크 면적비로 정량하였다. 회수율은 $91{\sim}101%$이었으며, 정량한계는 $1{\mu}g/kg$이었다. 벤젠의 평균검출수준은 과실음료의 경우 $5{\mu}g/kg$, 착향탄산음료의 경우 $2{\mu}g/kg$, 혼합음료의 경우 $7{\mu}g/kg$, 추출음료의 경우 $7{\mu}g/kg$이었다.

Keywords

References

  1. 식품저널 편집부 : 제3장 식품산업현황 in 식품유통연감 2005 (2005)
  2. Food and Drug Administration (FDA): Consumer magazine Sept-Oct (2005) www.kfa.gov/fdac/features/2006/506M_benzene.html. Assessed on Agust 31, 2007
  3. Barshick, S., Smith, S., Buchanan, M., Guerin, M. : Determination of benzene contenet in food using a novel blender purge and trap GC/MS method. J. Food Com. Anal., 9, 244-257 (1995)
  4. 식품의약품안전청 : 식품별 기준 및 규격 in 식품공전 pp. 281-296. (2005)
  5. Maltoni, C., Scarnato, C. : First experimental demonstration of the carcinogenic effect of benzene. Med. Lavoro, 70, 352-357 (1979)
  6. Halliwell, B., Gutteridge. J. : The importance of free radical catalytic metal ions in human disease. Mol. Aspects Med., 8, 89-193 (1985) https://doi.org/10.1016/0098-2997(85)90001-9
  7. Kuang, S., Liang. W.: Clinical analysis of 43 cases of cvhronic benzene poisoning. Chemico-Biological Interaction., 153-154, 129-135 (2005)
  8. Brandao. M., Rego, M., Pugliese, L., Clarencio, J., Bastos, C., Ferreira, J., Meyer, R., Neves, M., Freire. S. : Phenotype analysis of lymphocytes of workers with chronic benzene poisoning. Immunology Letters., 101, 65-70 (2005) https://doi.org/10.1016/j.imlet.2005.04.004
  9. International Agency for Research on Cancer (IARC): Summaries & Evaluations-Benzene. 29, p. 93 (1982)
  10. Fabietti, F., Delise, M., Bocca, A. : Investigation into the benzene and toluene content ofg soft drinks. Food Cont., 12, 505-509 (2001) https://doi.org/10.1016/S0956-7135(01)00041-X
  11. US Environmental Protect Agency (EPA): Method 1624 Revision B-Volatile organic compounds by isotope dilution GC/MS (Appendix A to Part 136) (1989)
  12. Przyjazny, A., Kokosa, J. : J. Chromatogr. A, 977, 143-153 (2002) https://doi.org/10.1016/S0021-9673(02)01422-X
  13. Gardner, L., Lawrence, G. : Short communications: Benzene production from decarboxylation of benzoic acid in the presence of ascorbic acid and a transition-metal catalyst. J. Agric. Food Chem. 41, 693-69 (1993) https://doi.org/10.1021/jf00029a001
  14. McNeal, T., Nyman, P., Diachenko, G., Hollifield, H. : Survey of benzene in foods by using headspace concentration techniques and capillary gas chromatography. J. AOAC Intl., 76, 1213-1219 (1993)
  15. Page B., Conacher H., Weber D, Lacroix G (1992) A survey of benzene in fruits and retail fruit juices, fruit drinks, and soft drinks. J. AOAC Intl., 75, 334-340 (1992)
  16. Creaser, C., Weston. D., Wilkins, J., Yorke, C., Irwin, J., Smith, B. : Determination of benzene in aqueous samples by membrane inlet, solid phase microextraction and purge and trap extraction with isotope dilution gas chromatographymass spectrometry. Anal. Commun., 36, 383-386 (1999) https://doi.org/10.1039/a908037f