Viability of Somatic Cell Nuclear Transfer Embryos following Embryo Transfer in Korean Native Striped Cattle (Bos namadicus Falconer, Chikso)

  • Published : 2007.12.31

Abstract

This study was conducted to examine the viability of Korean native striped cattle (Bos namadicus Falconer, Chikso) clone embryos after embryo transfer. Chikso somatic cell nuclear transfer (SCNT) embryos were produced by fusion of ear skin cells derived from a female Chikso with enucleated oocytes matured in vitro for 18-24 hr. After in vitro culture of SCNT embryos for 7 to 8 days, fresh or vitrified blastocysts derived from SCNT were transferred into a uterine horn of recipient cows. Fifteen of total 43 recipients were pregnant at Day 50 and 4 recipients were maintained to term. Three IVF-derived calves and 1 clone Chikso calf were born. Pregnancy rate was higher when fresh embryos were transferred to recipients compared to vitrified embryos, but development to term was not different between both groups. The clone Chikso calf died at 5 days after birth due to the fullness of amniotic fluid in rumen and the infection of umbilical cord. The result of the present study shows that clone Chikso calf can produced from the embryo transfer of SCNT embryos, however, solution of abortion problem is necessary to improve the cloning efficiency.

Keywords

References

  1. Baguisi A, Behboodi E, Melican DT, Pollock JS, Destrempes MM Cammuso C, Williams IL, Nims SD, Porter CA, Midura P, Palacios MJ, Ayres SL, Denniston RS, Hayes ML, Ziomek CA, Meade HM, Godke M Gavin WG, Overstrom EW, Echelard Y (1999): Production of goats by somatic cell nuclear transfer. Nat Biotechnol 17:456-461 https://doi.org/10.1038/8632
  2. Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Abel Ponce de Leon F, Robl JM (1998): Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280:1256-1258 https://doi.org/10.1126/science.280.5367.1256
  3. Dominko T, Chan A, Simerly C, Luetjens CM, Hewitson L, Martinovich C, Schatten G (2000): Dynamic imaging of the metaphase II spindle and maternal chromosomes in bovine oocytes: implications for enucleation efficiency verification, avoidance of parthenogenesis, and successful embryogenesis. Biol Reprod 62:150-154 https://doi.org/10.1095/biolreprod62.1.150
  4. Galli C, Lagutina I, Crotti G, Colleoni S, Turini P, Ponderato N, Duchi R, Lazzari G (2003): Pregnancy: A cloned horse born to its dam twin. Nature 424:635
  5. Kang YK, Koo DB, Park JS, Choi YH, Chung AS, Lee KK, Han YM (2001): Aberrant DNA methylation of donor genome in cloned bovine embryos. Nature Gen 28:173-177 https://doi.org/10.1038/88903
  6. Kato Y, Tani T, Sotomaru Y, Kurokawa K, Kato J, Doguchi H, Yasue H, Tsunoda Y (1998): Eight calves cloned from somatic cells of a single adult. Science 282:2095-2098 https://doi.org/10.1126/science.282.5396.2095
  7. Kubota C, Yamakuchi H, Todoroki J, Mizoshita K, Tabara N, Barber M, Yang X (2000): Six cloned calves produced from adult fibroblast cells after long-term culture. Proc Natl Acad Sci USA 97:990-995
  8. Kwon DJ, Park JH, Park CK, Yang BK, Cheong HI (2007): Survivability of in vitro fertilized and somatic cell nuclear transfer bovine embryos following vitrification. Reprod Dev Biol 31:29-33
  9. Lai L, Kolber-Simonds D, Park KW, Cheong HI, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ, Prather RS (2002): Production of $\alpha$ -1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295:1089-1092 https://doi.org/10.1126/science.1068228
  10. Lanza RP, Cibelli J, Diaz F, Moraes C, Farin PW, Farin CE, Hammer CJ, West MD, Namiani P (2000): Cloning of an endangered species using interspecies nuclear transfer. Cloning 2:79-90 https://doi.org/10.1089/152045500436104
  11. Lee BC, Kim MK, Jang G, Oh HI, Yuda F, Kim HI, Hossein Shamim M, Kim IT, Kang SK, Schatten G, Hwang WS (2005): Dogs cloned from adult somatic cells. Nature 436:641 https://doi.org/10.1038/436641a
  12. Loi P, Ptak G, Barboni B, Fulka J, Cappai P, Ginton M (2001): Genetic rescue of an endangered mammal by cross-species nuclear transfer using postmortem somatic cells. Net Biotechnol 19:962-964 https://doi.org/10.1038/nbt1001-962
  13. Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball S, Dai Y, Boone J, Walker 5, Ayares DL, Colman A, Campbell KHS (2000): Cloned pigs produced by nuclear transfer from adult somatic cells. Nature 407:86-90 https://doi.org/10.1038/35024082
  14. Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scoot AR, Ritchie M, Wilmut I, Colman A, Campbell KHS (1997): Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science 278:2130-2133 https://doi.org/10.1126/science.278.5346.2130
  15. Shiga K, Fujja T, Hirose Y, Sasue Y, Nagai T (1999): Production of calves by transfer of nuclei from culture somatic cells obtained from Japanese black bulls. Theriogenology 52:527-535 https://doi.org/10.1016/S0093-691X(99)00149-1
  16. Shin T, Kraemer D, Pryor J, Liun L, Rugila J, Howe L, Buck 5, Murphy K, Lyons L, Westhusin M (2002): A cat cloned by nuclear transplantation. Nature 415: 859 https://doi.org/10.1038/nature723
  17. Smith LC, Murphy BD (2004): Genetic and epigenetic aspects of cloning and potential effects on offspring of cloned mammals. Cloning Stem Cells 6:126-132 https://doi.org/10.1089/1536230041372319
  18. Vignon X, Chesne P, LeBourhis DJ, Heyman Y, Renard JP (1998): Developmental potential of bovine embryos reconstructed with somatic nuclei from cultured skin and muscle fetal cells. Theriogenology 49:392(abstr)
  19. Wakayama T, Perry ACF, Zuccotti M, Johnson KR, Yanagimachi R (1998): Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394:369-374 https://doi.org/10.1038/28615
  20. Wells DN, Misica PM, Day AM, Tervit HR (1997): Production of cloned lambs from an established embryonic cell line : A comparison between in vivoand in vitro matured cytoplasts. Biol. Reprod. 57: 385-393 https://doi.org/10.1095/biolreprod57.2.385
  21. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS (1997): Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813 https://doi.org/10.1038/385810a0
  22. Woods GL, White KL, Vanderwall DK, Li GP, Aston KI, Bunch TD, Meerdo LN, Pate BJ (2003): A mule cloned from fetal cells by nuclear transfer. Science 301:1063 https://doi.org/10.1126/science.1086743
  23. Xue F, Tian XC, Cubota C, Du F, Taneja M, Dinnyes A, Dai Y, Levine H, Pereira LV, Yang X (2002): Aberrent X-chromosome inactivation in deceased cattle derived from somatic cloning. Nature Gen 31:216-220 https://doi.org/10.1038/ng900
  24. Zakhartchenko V, Schemthaner W, Prelle K, Stojkovic P, Brem G, Wolf E (1999): Nuclear transfer in the bovine embryo: developmental potential of cultured adult cells. Theriogenology 51:218(abstr)