한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제22권4호
  • /
  • Pages.245-249
  • /
  • 2007
  • /
  • 2508-755X(pISSN)
  • /
  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지

Effect of Concentration and Exposure Duration of FBS on Parthenogenetic Development of Porcine Follicular Oocytes

  • Kim, Hyun-Jong (Animal Genetic Resources Station, NLAI, RDA) ;
  • Cho, Sang-Rae (Animal Genetic Resources Station, NLAI, RDA) ;
  • Choe, Chang-Yong (Animal Genetic Resources Station, NLAI, RDA) ;
  • Choi, Sun-Ho (Animal Genetic Resources Station, NLAI, RDA) ;
  • Son, Dong-Soo (Animal Genetic Resources Station, NLAI, RDA) ;
  • Kim, Sung-Jae (Animal Genetic Resources Station, NLAI, RDA) ;
  • Sang, Byung-Don (Animal Genetic Resources Station, NLAI, RDA) ;
  • Han, Man-Hye (Animal Genetic Resources Station, NLAI, RDA) ;
  • Ryu, Il-Sun (Animal Genetic Resources Station, NLAI, RDA) ;
  • Kim, In-Cheul (Animal Genetic Resources Station, NLAI, RDA) ;
  • Kim, Il-Hwa (Department of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Woon-Kyu (Department of Laboratory Animal Medicine, Yonsei University) ;
  • Im, Kyung-Soon (Department of Animal Biotechnology, Seoul National University)
  • 발행 : 2007.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The aim of present experiment was to examine hatching rate as in vitro indicator of viability of porcine embryos before early stage embryo transfer such as zygotes or 2-cell stage embryos. Cumulus-oocyte complexes (COCs) collected from ovaries were matured in North Carolina State University 23 (NCSU-23) containing 10% porcine follicular fluid (pFF), 10 ng/ml epidermal growth factor (EGF), $10{\mu}g/ml$ follicle stimulating hormone (FSH), $35{\mu}g/ml$ luteinizing hormone (LH), and 1mg/ml cysteine. After 24 hours, the COCs were transferred to the same medium without hormones. After 65h of maturation, oocytes were exposed to phosphate buffered saline (PBS) with 7% ethanol (v/v) for 7 minutes, and then the oocytes were washed and cultured in tissue culture medium (TCM) 199 containing 5 ug/ml cytochalasin B for 5h at $38.5^{\circ}C$ in an atmosphere of 5% $CO_2$ and 95% air with high humidity. After cytochalasin B treatment, the presumptive parthenotes were cultured in porcine zygote medium (PZM)-5 and cleavage of the parthenotes was assessed at 72h of activation, Normally cleaved parthenotes were cultured for an additional 8 days to evaluate their ability to develop to blastocyst and hatching stages. The fetal bovine serum (FBS) were added at Day 4 or 5 with concentrations of 2.5, 5 or 10%. The blastocyst rates were ranged within $39.1{\sim}70%$ in each treatment. However hatching rate was dramatically decreased in non-addition group. In this experiment, embryo viability in female reproductive tract may be estimated before embryo transfer with in vitro culture adding FBS by hatching ability.

키워드

참고문헌

  1. Balaban B, Urman B, Sertac A, Alatas C, Aksoy Sand Mercan R. 2000. Blastocyst quality affects the success of blastocyst-stage embryo transfer. Fertil. Steril. 74:282-287 https://doi.org/10.1016/S0015-0282(00)00645-2
  2. Balaban B and Urman B. 2003. Embryo culture as a diagnostic tool. Reprod. Biomed. Online. 7:671-682 https://doi.org/10.1016/S1472-6483(10)62090-1
  3. Bavister BD. 1995. Culture of pre implantation embryos: Facts and artifacts. Hum. Reprod. Update 1:191-148
  4. Carolan C, Lonergan P, Van Langendonckt A and Mermillod P. 1995. Factors affecting bovine embryo development in synthetic oviduct fluid following oocytes maturation and fertilization in vitro. Theriogenology 43: 1115-1128 https://doi.org/10.1016/0093-691X(95)00075-J
  5. Choi YH, Lee BC, Lim JM, Kang SK and Hwang WS. 2002. Optimization of culture medium for cloned bovine embryos and its influence on pregnancy and delivery outcome. Theriogenology 58:1187-1197 https://doi.org/10.1016/S0093-691X(02)00946-9
  6. Cui XS, Jeong YJ, Lee HY, Cheon SH and Kim NH. 2004. Fetal bovine serum influences apoptosis and apoptosis-related gene expression in porcine parthenotes developing in vitro. Reproduction. 127: 125-130 https://doi.org/10.1530/rep.1.00039
  7. Dobrinsky JR, Johnson LA and Rath D. 1996. Development of a culture medium (BECM-3) for porcine embryos: effects of bovine serum albumin and fetal bovine serum on embryo development. Biol. Reprod. 55:1069-1074 https://doi.org/10.1095/biolreprod55.5.1069
  8. Gardner DK and Lane M. 1997. Culture and selection of viable blastocysts: a feasible proposition for human IVF? Hum. Reprod. 3:367-382
  9. Gupta MK, Uhm SJ, Han DW and Lee HT. 2007. Embryo quality and production efficiency of porcine parthenotes is improved by phytohemagglutinin. Mol. Reprod. Dev. 74: 435-44 https://doi.org/10.1002/mrd.20547
  10. Kaaekuahiwi MA and Menino Jr AR. 1990. Relationship between plasminogen activator production and bovine embryo development in vitro. J. Anim. Sci. 68:2009-2014 https://doi.org/10.2527/1990.6872009x
  11. Kim HJ, Choi SH, Han MH, Son DS, Ryu IS, Kim IC, Lee JH, Kim IH, Im KS and Cho SR. 2005. Effects of maturation duration and activation treatments on activation and development of porcine follicular oocytes. Korean J. Emb. Trans. 20:25-33
  12. Kim HJ, Choi SH, Son DS, Cho SR, Choe CY, Kim YK, Han MH, Ryu IS, Kim IC, Kim IH, 1m KS and Nagai T. 2006. Effect of exposure duration of ovaries and oocytes at ambient temperature on parthenogenetic development of porcine follicular oocytes. J. Reprod. Dev. 52:633-8 https://doi.org/10.1262/jrd.17103
  13. Kim HS, Lee GS, Hyun SH, Lee SH, Nam DH, Jeong YW, Kim S, Kang SK, Lee BC, Hwang WS. 2004. Improved in vitro development of porcine embryos with different energy substrates and serum. Theriogenology 61:1381-1393 https://doi.org/10.1016/j.theriogenology.2003.08.012
  14. Men H, Agca Y, Critser ES, Critser JK. 2005. Beneficial effects of serum supplementation during in vitro production of porcine embryos on their ability to survive cryopreservation by open pulled straw vitrification. Theriogenology 64:1340-1349 https://doi.org/10.1016/j.theriogenology.2005.02.013
  15. Menino Jr AR and Williams JS. 1987. Activation of plasminogen by the early bovine embryo. Biol. Reprod. 36:1289-1295 https://doi.org/10.1095/biolreprod36.5.1289
  16. Nomura M, Iwase A, Furui K, Kitagawa T, Matsui Y, Yoshikawa M and Kikkawa F. 2007. Preferable correlation to blastocyst development and pregnancy rates with a new embryo grading system specific for day 3 embryos. J. Assist. Reprod. Genet. 24:23-28 https://doi.org/10.1007/s10815-006-9086-5
  17. Okada K, Krylov V, Kren R and Fulka Jr. J. 2006. Development of pig embryo after electro-activation and in vitro fertilization in PZM-3 or PZM supplemented with fetal bovine serum. J. Reprod. Dev. 52:91-98 https://doi.org/10.1262/jrd.17059
  18. Pinyopummintr T and Bavister BD. 1991. In vitro-matured/in vitro-fertilized bovine oocytes can develop into morulae/ blastocysts in chemically defined, protein-free culture media. Biol. Reprod. 45:736-742 https://doi.org/10.1095/biolreprod45.5.736
  19. Prather RS, Hawley RJ, Carter DB, Lai L and Greenstein JL. 2003. Transgenic swine for biomedicine and agriculture. Theriogenology 59: 115-123 https://doi.org/10.1016/S0093-691X(02)01263-3
  20. Robl JM and Davis DL. 1981. Effects of serum on swine morulae and blastocysts in vitro. J. Anim. Sci. 52:1450-1456 https://doi.org/10.2527/jas1981.5261450x
  21. Thompson JG. 1997. Comparison between in vivo-derived and in vitro-produced pre-elongation embryos from domestic ruminants. Reprod. Fertil. Dev. 9:341-354 https://doi.org/10.1071/R96079
  22. Thompson JG, Allen NW, McGowan LT, Bell ACS, Lambert MG and Tervit HR. 1998. Effect of delayed supplementation of fetal calf serum to culture medium on bovine embryo development in vitro and following transfer. Theriogenology 49: 1239-1249 https://doi.org/10.1016/S0093-691X(98)00071-5
  23. Urman B, Yakin K, Ata B and Balaban B. 2007. How can we improve current blastocyst grading systems? Curro Opin. Obstet. Gynecol. 19:273-278 https://doi.org/10.1097/GCO.0b013e3280bad854
  24. Wang WH, Abeydeera LR, Han YM, Prather RS and Day BN. 1999. Morphological evaluation and actin filament distribution in porcine embryos produced in vitro and in vivo. Biol. Reprod. 60:1020-1028 https://doi.org/10.1095/biolreprod60.4.1020
  25. Wang WH and Day BN. 2002 Development of porcine embryos produced by IVM/IVF in a medium with or without protein supplementation: effects of extracellular glutathione. Zygote 10: 109-115 https://doi.org/10.1017/S0967199402002150