Journal of environmental and Sanitary engineering (환경위생공학)

Korean Society for Environmental Sanitary Engineers (대한환경위생공학회)
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Microscopic observation of Paecilomyces lilacinus that have control over Meloidogyne incognita eggs

Meloidogyne incognita 알을 제어하는 Paecilomyces lilacinus의 현미경적 관찰

  • 이광배 (대구보건대학 임상병리과)
  • Published : 2007.09.30
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Abstract

M. incognita are plant-parasite nematode that cause severe damage to the crops. P. lilacinus are renowned for inhibitation of development of M. incognita's egg. We make a study for enzymatic examining the cause closely that P. lilacinus suppress development of M. incognita's egg by parasiting. The research result is explained the place below. 1. The egg that is exposed to co-enzymes which are cultured in the broth culture starts to change the membrane of egg from 3days. And in 10days, that membrane completely disappear. These are observed through light microscope. Therefore, we know that M. incongnita are controlled by extracellular lytic enzymes that are produced by P. lilacinus. 2. Through scanning electron microscope, we can find that the egg that is attacked by P. lilacinus loses it's membrane gradually, and that loss of the membrane causes transform, which suppresses the development of egg.

Keywords

References

  1. 최영연. 추호열.1978. 경제잘물에 영향을 미치는 뿌리혹선충에 관한 연구
  2. Williams., 'Tbird Generation pesticides'in man and Ecosphere, Reading from Scientific American. W.H. freeman Co., San Francisco, calif.,1971
  3. Kerry, B.R. 1990. An assessment of progress toward microbial control of plant-parasitic nematode. Supplment to J. Nematol. 22(4S) : 621-631
  4. Zari, E. A.and D.S. Bhatti. 1990. In VIVO parasitism of Melodiogyne javanica by an ovipparasitic Fungus, Paecilomyces lilacinus. nematol medict 8 : 141-143
  5. Zaki, F. A.,and D.S bhatti. 1988. Economical method for mass culturing of PiIlacinus A-267. J.Chem.Soc.Comnun., 94-96
  6. Stirling,G.R. 1984, Biological control of with Bacillus penetrans. Phytopathology 74:55-60 Meloidogyne incognita https://doi.org/10.1094/Phyto-74-55
  7. Striling,C.R. and mankau.R 1979.Mode of parasitism of meloidogyne and other nematode eggs by Daetylella oviparasitical.J. Nematol.11.82-288
  8. Dunn, M.T.,R.M Sayre A. Carrell, ang W. P. Wergin 1982. Colonization of nematode egg by P.lilacinus observed with microscope. SEM.111(1)
  9. Caveness, F.E. and Jensen H.J. 1955. Modification of the centrifugal floation technique for the isolation and concentration of nematode and their egg from soil and plant tissue. Proc. Helminth. Soc. Wash
  10. Parasad,S.V.,B.R TIlak and K.G.Gollaakota. 1972. Role of Bacillus thuringiensis var. thuringiensis on the larval surviability and egg hatching of Meloidogyne spp., the causative agent of root-knot disease. J.Inverteber. Pathol.20:377 -378 https://doi.org/10.1016/0022-2011(72)90177-2
  11. Domsch K.H., Gams W. and Anderson T.H., (Eds)1980. Compendium of soil fungi. Vol.1. London Academic Press
  12. Jatala P., Katenback and M. bocangel. 1979. Biological control of Meloidogyne incognita acrita and Globodera pallida on potatoes. j. Nematol. 11 :303
  13. J. S. Price and R Storck 1075. Production, Purification, and Characterization of an Exteracellular Chitosanase from P. lilacinus
  14. Jatala, P., Franco, A. Gonzalez. Journal of Nematology. Vol.17(4) 1985. hatching Stimulation and inhibition of Globodera pallida egg by the enzymatic and expopathic toxic compound of some biocontrol fungi