Animal cells and systems

  • 제11권2호
  • /
  • Pages.183-189
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  • 2007
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  • 1976-8354(pISSN)
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  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Mucosubstance Histochemistry of the Epidermis in Yellowtail, Striped Beakperch, Brown Spotted Grouper, Sea Chub, and Multicolorfin Rainbowfish

  • Jeong, Gil-Nam (Biology Education Major, Division of Science Education, College of Education, Pusan National University) ;
  • Jo, Un-Bock (Biology Education Major, Division of Science Education, College of Education, Pusan National University)
  • 발행 : 2007.12.31
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초록

To investigate the properties of mucosubstances of the epidermis in various teleostean species, conventional histochemical stainings were used on the skin in five species of order Perciformes, i. e., yellowtail, Seriota quinqueradiat, striped beakperch, Oplegnathus fasciatus, brown spotted grouper, Epinephelus chlorostigma, sea chub, Ditrema temmincki and multicolorfin rainbowfish, Halichoeres poecilopterus. The following methods were used: periodic acid Schiff (PAS), alcian blue (AB) pH at 2.5, AB pH at 1.0, AB pH at 2.5-PAS, AB pH at 1.0-PAS, aldehyde fuchsin (AF) pH at 1.7-AB pH at 2.5 and high iron diamine (HID)-AB pH at 2.5. The epidermis of all five species consisted of three layers: superficial, middle, and basal layer. The superficial layer was comprised of rather flattened cells. In particular, the outermost layer of striped beakperch and middle layer of sea chub consisted of mucus-secreting cells. Mucous cells, the unicellular glands, were found in epidermis but varied in number in different body regions and species. Although there was a slight difference in the amount in various species and body regions, the secretory contents of the mucous cells in the five teleostean species contained acidic mucopolysaccharides. In yellowtail, striped beakperch, and multicolorfin rainbowfish, the property of mucosubstances was identified as sialomucin, while it was sulphomucin in brown spotted grouper and sea chub.

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참고문헌

  1. Alexander JB and Ingram GM (1992) Noncellular nonspecific defense mechanisms of fish. Annu Rev Fish Dis 3: 249-279
  2. Blackstock N and Pickering AD (1982) Changes in the concentration and histochemistry of epidermal mucous cells during the alevin and fry stages of the brown trout, Salmo trutta. J Zool 197: 463-471 https://doi.org/10.1111/jzo.1982.197.4.463
  3. Cole AM, Weis P and Diamond G (1997) Isolation and characterization of pleurocidin, and antimicrobial peptide in the skin secretions of winter flounder. J Biol Chem 272: 1208-1213
  4. Fletcher TC and White A (1973a) Lysozyme activity in plaice (Pleuronectes platessa L.). Experientia 29: 1283-1285 https://doi.org/10.1007/BF01935119
  5. Fletcher TC and White A (1973b) Antibody production in the plaice Pleuronectes platessa after oral and parenteral immunisation with Vibrio anguillarum antigens. Aquaculture 1: 417-428 https://doi.org/10.1016/0044-8486(72)90045-2
  6. Ingram GA (1980) Substances involved in the natural resistances offish to infection-a review. J Fish. Biol 16: 23-60 https://doi.org/10.1111/j.1095-8649.1980.tb03685.x
  7. Jeong YK and Moon MJ (1994) Integumental secretory cells in goldfish, Carassius auratus. L. Korean J Electron Microscopy 24: 1-10
  8. Kazuyori Y and Motoyoshi Y (1975) Morphochemical analysis of mucosubstances in some epithelial tissues of the eel (Anguillajaponica). Histochemistry 43: 161-172 https://doi.org/10.1007/BF00492444
  9. Kim JW, Chin P, Jin YG and Lee JS (2002) Ultrastructure on the integumentary epidermis of the bastard halibut, Paralichthys olivaceus (Teleostei: Pleuronectidae). Korean J Electron Microscopy 32(2): 121-129. (in Korean)
  10. Lee JS and Kim JW (1999) Fine structure of the integumentary supporting cell and gland cell of the sea bass, Lateolabrax japonicus (Teleostei: Moronidae). Korean J Electron Microscopy 29(4): 417-426. (in Korean)
  11. Lee JS, An CM and Huh SH (2000a) Integumentary ultrastructure of the blenny, Pholis nebulosa (Teleostei: Pholidae). J Korean Fish Soc 33: 148-152
  12. Lee JS, Kang JC and Baek HJ (2000b) Ultrastructure on the integumentary epidermis of the marbled sole, Limanda yokohamae (Teleostei: Pleuronectidae). Korean J Electron Microscopy 30: 303-310
  13. Lev R and Spicer SS (1964) Specific staining of sulfated group with alcian blue at low pH. J Histochem Cytochem 12: 309 https://doi.org/10.1177/12.4.309
  14. McManus JFA (1968) Periodic acid Schiff's reaction. Stain Techn 23: 99-108, 1948 (AFIP modification). Copyright by manual of histologic staining methods of the armed forces
  15. Mittal AK and Baneljee TK (1974) Structure and keratinization of the skin of a fresh-water teleost Notopterus notopterus. J Zool Lond 174: 341-355 https://doi.org/10.1111/j.1469-7998.1974.tb03162.x
  16. Mittal AK and Munshi JSD (1971) A comparative study of the structure of the skin of certain air-breathing fresh-water teleosts. J Zool Lond 163: 515-532 https://doi.org/10.1111/j.1469-7998.1971.tb04547.x
  17. Mittal AK, Veda T, Fujimori O and Yamada K (1994) Histochemical analysis of glycoproteins in the epidermal mucous cells and sacciform cells of an Indian swamp eel, Monopterus cuchia (Hamilton) (Synbranchiformes, Pisces). Acta Histochem Cytochem 27(3): 193-204 https://doi.org/10.1267/ahc.27.193
  18. Mittal AK, Whitear M and Agarwal SK (1980) Fine structure and histochemistry of the epidermis of the fish, Monopterus cuchia. J Zool Lond 191: 107-125 https://doi.org/10.1111/j.1469-7998.1980.tb01452.x
  19. Moore JD, Ototake M and Nakanishi T (1998) Particulate antigen uptake during immersion immunisation of fish: the effectiveness of prolonged exposure and the roles of skin and gill. Fish Shellfzsh Immunol 8: 393-407 https://doi.org/10.1006/fsim.1998.0143
  20. Mowry RW (1963) The special value or method that color both acidic and vicinal hydrate groups in the histochemical study of mucin. Ann NY A cad Sci 106: 402-423
  21. Ottsen OH and Olafsen JA (1997) Ontogenetic development and composition of the mucous cells and the occurrence of saccular cells in the epidermis of Atlantic halibut. J Fish Biol 50: 620-633 https://doi.org/10.1111/j.1095-8649.1997.tb01954.x
  22. Park IS, Kim JJ, Jo VB, and Park SO (1995) Fine structure changes in the eel epidermis according to sea water adaptation. II. Mucous cell and club cell. Korean J Zool 38: 38-47
  23. Park JY and Kim IS (1999) Structure and histochemistry of skin of mud loach, Migurnus anguillicaudatus (Pisces, Cobitidae), from Korea. Korean J Ichthyol 11: 109-116
  24. Park JY and Kim IS (2000) Structure and cytochemistry of skin in spined loach, Iksookimia longicorpus (Pisces, Cobitidae). Korean J Ichthyol 12: 25-32
  25. Park JY and Kim IS (2003) Skin and its gland cells a nemacheiline loach, Orthrias nudus (Pisces: Balitoridae) in Korea. Korean J IchthyoI 15(1): 19-25
  26. Park JY, Kim IS and Kim SY (2001) Morphology and histochemistry of the skin of the mud loach, Misgurnus mizolepis, in relation to cutaneous respiration. Korean J Biol Sci 5: 303-308 https://doi.org/10.1080/12265071.2001.9647619
  27. Park JY, Kang EJ and Kim YC (2002) Structural and Histochemical study on the skin of a Korean bullhead, Pseudobagrusfulvidraco. Korean J IchthyoI 14(4): 247-253
  28. Park JY, Kim IS, Lee YJ and Baek HA (2005) Mucous cells and their structure on the epidermis of five appendages in the korean flat-headed goby, Luciogobius guttatus(Pisces; Perciformes). Korean J IchthyoI 17(3): 167-172
  29. Sato M (1979) Fine structure of the small and large mucous cells found in the skin epidermis of two cottids, Pseudoblennius cotto ides and Furcina sp. Jap J IchthyoI 26(1): 75-83
  30. Shephard KL (1994) Functions for fish mucus. Rev Fish Biol Fish 4(4): 401-429 https://doi.org/10.1007/BF00042888
  31. Singh SK and Mittal AK (1990) A comparative study of the epidermis of the common carp and the three indian major carp. J Fish Biol 36: 9-19 https://doi.org/10.1111/j.1095-8649.1990.tb03515.x
  32. Solanki TG and Benjamin M (1982) Changes in the mucous cells of the gills, buccal cavity and epidermis of the nine-spined stickleback, Pungitus pungitus L., induced by transferring the fish to sea water. J Fish Biol 21: 563-575 https://doi.org/10.1111/j.1095-8649.1982.tb02860.x
  33. Spicer SS (1965) Diamine methods for differentiating mucosubstances histochemically. J Histochem Cytochem 13(3): 211-234 https://doi.org/10.1177/13.3.211
  34. Spicer SS and Meyer DB (1960) Histochemical differentiation of acid mucopolysaccharides by means of combined aldehyde fuchsin-alcian blue staining. Am J Clin Pathol 33: 454-460
  35. Spicer SS, Horn RG and Leppi TJ (1967) Histochemistry of connective tissue mucopolysaccharides. In: Wagner BM and Smith DE (eds), The connective tissue, Williams & Wilkins, Baltimore, pp 251-303
  36. Whiteal M (1986) The skin of fishes including cyclostomes: epidermis. In: (Bereiter-Hahn J, Matoltsy AG, and Richards KS (eds), Biology of the integument Vol. 2 Vertebrates, Springer Verlag, New York, pp 9-64
  37. Whitear M and Mittal AK (1986) Structure of the skin of Agonus cataphractus (Teleostei). J Zool Lond A 210: 551-574
  38. Zaccone GP, Kapoor BG, Fasulo Sand Ainis L (2001) Structural, histochemical and functional aspects of the epidermis of fishes. Adv Mar Biol 40: 243-248