배무채(xBrassicoraphanus)의 화학성분 분리

Isolation of Chemical Compounds from xBrassicoraphanus

  • 발행 : 2007.12.31

초록

xBrassicoraphanus is an intergenic breed crossed between Brassica campetris L. ssp. pekinensis and Raphanus sativus L. that have been daily consumed. xBrassicoraphanus was known to have good tastes and biological activities. Nevertheless, its constituetnts were not elucidated yet. Thus, in the present study, to indirectly evaluate the biological activity of xBrassicoraphanus, 12 compounds were isolated from leaves and roots of xBrassicoraphanus. On the basis of spectroscopic evidences, the structures of these compounds isolated from leaves of xBrassicoraphanus. were identified as ${\beta}-sitosterol$, indole-3-acetonitrile, ferulic acid, methyl ferulate, linolenic acid methyl ester, linolenic acid and coniferyl alcohol, while the chemical structures of compounds isolated from the roots of were xBrassicoraphanus were characterized as ${\beta}-sitosterol$, indole-3-acetonitrile, ferulic acid, methyl ferulate, linolenic acid methyl ester, 1-methoxyindole-3-acetonitrile, goitrin, 4-hydroxycinnamyl alcohol, coniferyl alcohol, palmitic acid and daucosterol. These can be classified as three steroids, two indole cyanides, two cinnamic acid derivatives, one cinnamyl alcohol derivative, three fatty acid derivatives one isothiocyanate. These results suggest that the compounds isolated from xBrassicoraphanus were almost identical with known components of Brassica campetris L. ssp pekinensis or Raphanus sativus L. However, it is necessary to investigate more about the difference of amounts of constituents according to harvest time and variant species amounts.

키워드

참고문헌

  1. Lee J.M. (2003) Vegetable Horticultures. Hynagmunsa. 324- 347
  2. Park K.W., Ryu K.O. (2000) Functional Vegetables. Herb World Press. 131
  3. Cheigh H.S., Park K.Y, (1994) Biochemical, morphological, and nutritional aspects of kimchi (korean fermaented vegetable products). Critical Reviews in Food Science and Nutrition 34(2): 175-203 https://doi.org/10.1080/10408399409527656
  4. Choi Y.H., Kim J.S., Seo J.H., Lee J.W., Kim Y.S., Ryu S.Y., Lee K.R., Kim Y.K., Kim S.H. (2004) Chemical constituents of Brassica campetris ssp pekinensis. Kor J Pharmacogn. 35(3): 255-258
  5. Lee S.S. (1999) Breeding a fertile intergeneric allotetraploid plant between heading Chinese cabbage and korean radish. Kor J Hort Sci Technol. 10: 653
  6. Ahuja K.L., Batta S.K., Raheja R.K., Labana K.S., Gupta M.L. (1989) Oil content and fatty acid composition of promising Indian Brassica campestris L. (Toria) genotypes. Plant Foods Hum Nutr. 39(2): 155-160 https://doi.org/10.1007/BF01091895
  7. Dias C., Dias M., Borges C., Almoster Ferreira M.A., Paulo A., Nascimento J. (2003) Structural elucidation of natural 2- hydroxy di- and tricarboxylic acids and esters, phenylpropanoid esters and a flavonoid from Autonoe madeirensis using gas chromatographic/electron ionization, electrospray ionization and tandem mass spectrometric techniques. J Mass Spectrometry 38(12): 1240-1244 https://doi.org/10.1002/jms.554
  8. Chowdhury B.K. and Chakraborty D.P. (1971) 3-formylindole from Murraya exotica. Phytochemistry 10: 481-483 https://doi.org/10.1016/S0031-9422(00)94088-3
  9. Harrison L.J., Sia G.L., Sim K.Y., Tan H.T., Connolly J.D., Lavaud C., Massiot G. (1995) A ferulic acid ester of sucrose and other constituents of Bhesa paniculata. Phytochemistry 38(6): 1497-1500 https://doi.org/10.1016/0031-9422(94)00314-J
  10. Pouchert C. (2004) Aldrich Library of $^{13}C$ and $^1H$ FT NMR Spectra, 3, 136A, 136B, 153C
  11. Kim J.S., Choi Y.H., Seo J.H., Lee J.W., Kim Y.S., Ryu S.Y., Kang J.S., Kim Y.K., Kim S.H. (2004) Chemical constituents of Brassica campetris ssp rapa. Kor J Pharmacogn. 35(3): 259-263
  12. Kenji M., Mitsuo T., Akira S. (1995) Three sulphur-containing stress metabolites from Japanese radish. Phytochemistry 39: 581-586 https://doi.org/10.1016/0031-9422(95)00011-U
  13. Loub W. D., Wattenberg L. W., Davis D. W. (1975) Aryl hydrocarbon hydroxylase induction in rat tissues by naturally occurring indoles of cruciferous plants. J Natl Cancer Inst. 54(4): 985-988