The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

The Cellulase and Pectinase Activities Associated with the Virulence of Indigenous Sclerotinia sclerotiorum Isolates in Jordan Valley

  • Asoufi, H. (IPM Program, National Center for Agricultural Research and Technology Transfer (NCARTT)) ;
  • Hameed, K.M. (Department of Plant Production, Jordan University of Science and Technology) ;
  • Mahasneh, A. (Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology)
  • Published : 2007.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Twenty five isolates of Sclerotinia sclerotiorum were recovered from different infested fields of vegetable along the heavily cultivated crops in Jordan valley. Cellulase and pectinase activities of those isolates were detected using CMC and pectin agar media, respectively. Diameter of the clearing zones on those media represented the level of such enzymatic activities, characteristic of each isolate. The virulence of those isolates was studied using a squash (Cucurbita pipo) cultivar under a greenhouse condition. The significance of correlating the enzymatic activity with the virulence of the isolates was ascertained and discussed.

Keywords

References

  1. Collmer, A. and Keen, N. 1986. The role of pectic enzymes in plant pathogenesis. Annu. Rev. Phytopathol. 24:383-409 https://doi.org/10.1146/annurev.py.24.090186.002123
  2. Deena, E. and Kohn, L. 1995. Comparison of pectic zymograms produced by different clones of S. sclerotiorum in culture. Phytopathology 85:292-298 https://doi.org/10.1094/Phyto-85-292
  3. Echandi, E. and Walker, J. 1957. Pectolytic enzymes produced by S. sclerotiorum. Phytopathology 47:303-307
  4. Godoy, G,. Steadman, J., Dickman, M. and Dam, R. 1990. Use of mutants to demonstrate the role of oxalic acid in pathogenicity of S. sclerotiorum on Phaseolus vulgaris. Physiol. and Mol. Plant Pathol. 37:179-191 https://doi.org/10.1016/0885-5765(90)90010-U
  5. Huntur, J., Dickson, M. and Cigha, J. 1981. Limited term inoculation: a method to screen bean plants for partial resistance to white mold. Plant Dis. 65:414-417 https://doi.org/10.1094/PD-65-414
  6. Imolehin, E., Grogan, R. and Duniway, J. 1980. Effect of temperature and moisture tension on growth, sclerotial production, germination, and infection by Sclerotinia minor. Phytopathology 70:1153-1157 https://doi.org/10.1094/Phyto-70-1153
  7. Lumesden, R. 1976. Pectolytic enzymes of Sclerotinia sclerotiarum and their localization of infected bean. Can. J. Bot. 54:2630-2641 https://doi.org/10.1139/b76-283
  8. Lumsden, R. 1979. Histology and physiology of pathogenesis in plant diseases caused by Sclerotinia species. Phytopathology 69:890-895 https://doi.org/10.1094/Phyto-69-890
  9. Mamluk, O., Qasern, S. and Skaria, M. 1980. The distribution and prevalence of fungal and bacterial disease of vegetables in Jordan. Dirasat. 17:203-211
  10. Maxwell, M. and Lumsden, R. 1970. Oxalic acid production by S. sclerotiorum in infected bean and in culture. Phytopathology 60: 1395-1398 https://doi.org/10.1094/Phyto-60-1395
  11. Morall, R., Deczek, J. and Sheard, J. 1972. Variation and correlation within and between morphology, pathogenicity, and pectolytic enzyme activity in Sclerotinia from Saskatchewan. Can. J. Bot. 50:767-786 https://doi.org/10.1139/b72-095
  12. Osofi, H., Hameed, K. and Mahaseneh, A. 2005. Relatedness among Indigenous Member of Sclerotinia sclerotiorum Under Jordan Valley. J. Plant Pathol. 21: 106-110 https://doi.org/10.5423/PPJ.2005.21.2.106
  13. Pudry, L. 1979. Sclerotinia sclerotiorum: history, diseases and symptomology, host range, geographic distribution, and impact. Phytopathology 69:875-880
  14. Tariq, V.. Gutteridge, C. and Jeffries, P. 1985. Comparative studies of cultural and biochemical characteristics used for distinguishing species within Sclerotinia. Trans. Br. Mycol. Soc. 84:381-397 https://doi.org/10.1016/S0007-1536(85)80001-2
  15. Varzea, V., Rodrigues, C. and Lewis, B. 2002. Distinguishing characteristics and vegetative comparability of Colletotrichum kahawein comparison with other related species from coffee. Plant Pathol. 51:202-207

Cited by

  1. Pepper pectin methylesterase inhibitor protein CaPMEI1 is required for antifungal activity, basal disease resistance and abiotic stress tolerance vol.228, pp.1, 2008, https://doi.org/10.1007/s00425-008-0719-z
  2. Molecular Identification of Spoilage Fungi Isolated from Fruit and Vegetables and Their Control with Chitosan vol.29, pp.2, 2015, https://doi.org/10.1080/08905436.2015.1027222
  3. EST profiling of resistant and susceptible Hevea infected by Microcyclus ulei vol.76, pp.2, 2011, https://doi.org/10.1016/j.pmpp.2011.07.006
  4. Extracellular enzymes activity determining the virulence of Rhizoctonia bataticola, causing root rot in soybean vol.100, 2017, https://doi.org/10.1016/j.pmpp.2017.06.003
  5. Invasion of Solanum tuberosum L. by Aspergillus terreus: a microscopic and proteomics insight on pathogenicity vol.7, pp.1, 2014, https://doi.org/10.1186/1756-0500-7-350
  6. The complexity of the Sclerotinia sclerotiorum pathosystem in soybean: virulence factors, resistance mechanisms, and their exploitation to control Sclerotinia stem rot pp.1983-2052, 2018, https://doi.org/10.1007/s40858-018-0259-4