Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
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Preparation and Characterization of Low Molecular Weight Water Soluble Chitosan Gene Carrier Fractioned according to Molecular Weight

저분자량 수용성 키토산이 분급화된 유전자 전달체의 제조 및 특성

  • Jang, Min-Ja (Department of Nano Polymer Science and Engineering, Sunchon National University) ;
  • Kim, Dong-Gon (Department of Nano Polymer Science and Engineering, Sunchon National University) ;
  • Jeong, Young-Il (Department of Nano Polymer Science and Engineering, Sunchon National University) ;
  • Jang, Mi-Kyeong (Department of Nano Polymer Science and Engineering, Sunchon National University) ;
  • Nah, Jae-Woon (Department of Nano Polymer Science and Engineering, Sunchon National University)
  • 장민자 (순천대학교 나노고분자공학과) ;
  • 김동곤 (순천대학교 나노고분자공학과) ;
  • 정영일 (순천대학교 나노고분자공학과) ;
  • 장미경 (순천대학교 나노고분자공학과) ;
  • 나재운 (순천대학교 나노고분자공학과)
  • Published : 2007.11.30
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Abstract

To obtain low molecular weight water soluble chitosan (LMWSC) with various molecular weights, chitosan oligosaccharides (COS) with lactic acid was separated by using ultrafilteration technique and LMWSC with a free amine group was prepared by the novel salts-removal method. The characterization of LMWSC removed the lactic acid and degree of deacetylation (DDA) were identified by FT-IR and $^1H-NMR$ spectra. Polydispersity index (PDI) was $1.278{\sim}1.499$, which indicates a relatively molecular weight distribution. To identify the potential as a gene carrier, we confirmed the transfection efficiency of COS fractioned according to molecular weight successfully and the salt-removed LMWSC using 293T cell. Also, LMWSC derivatives prepared for improvement transfection efficiency were evaluated using Balb/C mice.

다양한 분자량을 가진 저분자량 수용성 키토산을 얻기 위해 젖산염이 결합되어 있는 키토산 올리고당을 한외여과막 장치를 이용하여 분리하였고, 새로운 염 제거법으로 유리 아민기를 가진 LMWSC를 제조하였다. 젖산염이 제거된 LMWSC의 특성과 탈아세틸화도가 적외선 분광기(Infrared spectroscopy, IR) 및 핵자기공명장치($^1H-Nuclear$ Magnetic Resonance, $^1H-NMR$)에 의해 확인되었다. 분자량을 나타내는 다분산지수(PDI)는 $1.278{\sim}1.499$로 비교적 좁은 분자량분포를 나타내었다. 유전자 전달체로서의 가능성을 확인하기 위하여, 성공적으로 분자량에 따라 분리된 키토산 올리고당과 염이 제거된 LMWSC의 유전자 전이효율이 293T cell을 이용하여 확인하였다. 또한 유전자 전이효율을 향상시키기 위해 제조된 LMWSC 유도체가 Balb/C mice를 이용하여 평가되었다.

Keywords

References

  1. L. Huang, M. C. Hung, and E. Wagner, Nonviral vectors for gene therapy, Academic Press, New York, 1999
  2. M. Lee and S. W. Kim, Pharmaceut. Res., 22, 1 (2005) https://doi.org/10.1007/s11095-004-9003-5
  3. M. Ruponen, Y. Herruala, and A. Urtti, Biochimica et Biophysica Acta, 1415, 331 (1999)
  4. K. Y. Lee, I. C. Kwon, Y. H. Kim, W. H. Jo, and S. Y. Jeong, J. Control. Release, 51, 213 (1998)
  5. D. H. Seo, B. C. Shin, and M. S. Kim, Polymer(Korea), 29, 277 (2005)
  6. J. H. Felgner, R. Kumar, and C. N. Sridhar, J. Bio. Chem., 269, 2550 (1994)
  7. J. S. Kim, A. Maruyama, T. Akaike, and S. W. Kim, J. Control. Release, 47, 51 (1997)
  8. G. Y. Wu and C. H. Wu, Biochemistry, 27, 887 (1988)
  9. G. Y. Wu and C. H. Wu, J. Bio. Chem., 262, 4429 (1987)
  10. G. Y. Wu, J. M. Wilson, F. Shalaby, M. Grossman, D. A. Shafritz, and C. H. Wu, J. Bio. Chem., 266, 14338 (1991)
  11. K. Kunath, A. V. Harpe, D. Fisher, and T. Kissel, J. Control. Release, 88, 159 (2003) https://doi.org/10.1016/S0168-3659(02)00458-3
  12. T. Reschel, C. Konak, D. Oupicky, L. W. Seymour, and K. Ulbrich, J. Control. Release, 81, 201 (2002)
  13. R. Hejazi and M. Amiji, J. Control. Release, 89, 151 (2003) https://doi.org/10.1016/S0168-3659(03)00126-3
  14. M. Thanou, J. C. Verhoef, and H. E. Junginger, Adv. Drug Deliver. Rev., 52, 117 (2001) https://doi.org/10.1016/S0169-409X(01)00199-5
  15. K. Ogawa, T. Yui, and K. Okuyama, Int. J. Bio. Macromol., 34, 1 (2004) https://doi.org/10.1016/j.ijbiomac.2003.11.002
  16. W. G. Liu, X. Zhang, S. J. Sun, G. J. Sun, K. D. Yao, D. C. Liang, G. Guo, and J. Y. Zhang, Bioconjugate Chem., 14, 782 (2003) https://doi.org/10.1021/bc020051g
  17. Y. H. Kim, S. H. Gihm, C. R. Park, K. Y. Lee, T. W. Kim, I. C. Kwon, H. Chung, and S. Y. Jeong, Bioconjugste Chem., 12, 932 (2001) https://doi.org/10.1021/bc000176q
  18. J. W. Nah and M. K. Jang, J. Polym. Sci.; Part A: Polym. Chem., 40, 3796 (2002)
  19. C. Choi, M. K. Jang, and J. W. Nah, Polymer(Korea), 30, 279 (2006)
  20. C. Choi, D. G. Kim, M. J. Jang, T. H. Kim, M. K. Jang, and J. W. Nah, J. Appl. Polym. Sci., 102, 3545 (2006) https://doi.org/10.1002/app.24809
  21. M. Huisinga, K. Failing, and M. Reinacher, Vet. Immunol. Immunop., 118, 210 (2007) https://doi.org/10.1016/j.vetimm.2007.05.020