생명과학회지 (Journal of Life Science)

  • 제17권11호
  • /
  • Pages.1555-1561
  • /
  • 2007
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지
DOI QR코드

DOI QR Code

무기물성 및 식물성 생리활성 물질이 반추위 미생물의 성장에 미치는 영향

Effects of Biologically Active Materials Prepared for Several Minerals and Plants on the Growth of Rumen Microbes

  • 신성환 (경상대학교 응용생명과학부) ;
  • 이신자 (경상대학교 응용생명과학부) ;
  • 옥지운 (경상대학교 응용생명과학부) ;
  • 이상민 (경상대학교 응용생명과학부) ;
  • 임정화 (경상대학교 응용생명과학부) ;
  • 김경훈 (축산과학원 축산생명환경부 영양생리과) ;
  • 문여황 (진주산업대학교 동물생명과학과) ;
  • 이성실 (경상대학교 응용생명과학부)
  • Shin, Sung-Whan (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Lee, Shin-Ja (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Ok, Ji-Un (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Lee, Sang-Min (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Lim, Jung-Hwa (Division of Applied Life Science(BK 21), GyeongSang National University) ;
  • Kim, Kyoung-Hoon (National Institute of Animal Science, Animal Nutrition and Physiology Division) ;
  • Moon, Yea-Hwang (Department of Animal Sci. & Biotech, RAIC, Jinju National University) ;
  • Lee, Sung-Sill (Division of Applied Life Science(BK 21), GyeongSang National University)
  • 발행 : 2007.11.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

본 연구는 무기물성 및 식물성 유래 생리활성물질로서 scoria, germanium, charcoal, 생강, stevia 및 CLA(conjugated linoleic acid)가 병원성 미생물 및 반추위 미생물에 대한 작용을 조사하기 위하여 수행되었다. 병원성 균으로서 Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes 및 Staphylococcus aureus에 적용하여 항균활성을 측정하고, in vitro 발효 시 반추위 미생물 성장률, 가스 생성량, 암모니아 농도, CMCase 활성 및 미생물의 수를 측정하였다. 병원성 미생물 배양액에 생강을 0.1% 첨가한 구에서만 항균활성이 나타났으나, paper disc법에 의한 항균활성 시험에서는 stevia 10%첨가구와 CLA 10% 첨가구에서 E. coli에 대해서 항생제 첨가구인 positive control 구와 비슷한 크기의 clear zone을 형성하였다. in vitro 반추위 미생물 발효시험에서는 생강, stevia 및 CLA가 반추위 박테리아와 프로토조아의 증식을 억제하는 것으로 나타났는데, 특히 생강 첨가구의 경우 메탄 생성균의 서식지로 알려져 있는 프로토조아를 크게 억제함으로써 메탄생성 억제제로서 개발 가능성이 있는 것으로 사료된다.

In order to know the effects of scoria, germanium, charcoal, ginger, stevia, and CLA(Conjugated Linoleic Acid) as biologically active materials on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro lumen microbial growth, gas production, ammonia concentration, carboxymethyl-cellulase (CMCase) activity, and microbial populations were investigated. The growth of pathogenic microbes was inhibited by the supplement of 0.10% ginger. Ginger had powerful antimicrobial properties on all the pathogens used in this experiments. Additionally in the antibacterial assay by paper disc method, we could observe the clear zone of similar area with the positive control(antibiotics) for E. coli as applied with the 10% stevia or the 10% CLA only. The supplements of ginger, stevia and CLA in vitro rumen fermentation inhibited populations of rumen bacteria and protozoa. Particularly supplement of ginger resulted in remarkable reduction of the protozoa population, which means it might serve as a source inhibiting material of methane creation in the rumen.

키워드

참고문헌

  1. Abe, M., and F. Kumeno. 1972. Modifying method for counting protozoa. Jpn. J. Zootech. Sci. 43, 535-536.
  2. Baumgard, D. E., B. A. Corl, D. A. Dwyer, A. Saebo and D. E. Bauman. 2000. Identification of the conjugated linoleic acid isomer that inhibits milk fat synthesis. Ame. J. Phys. 278, 179-184.
  3. Belury, M. A., K. P. Nickel, C. E. Bird and Y. Wu. 1996. Dietary conjugated linoleic acid modulation of phorbol ester skin tumor promotion. Nutrition Canc. 26, 149-157. https://doi.org/10.1080/01635589609514471
  4. Benzamin, H., J. M. Strokson, K. Albright and M. W. Pariza. 1990. TPA-mediated induction of ormithine decarboxylase activity in mouse forestomach and its inhibition by conjugated dienoic derivatives of linoleic aicd. FASEB J. 4, A508.
  5. Bryant, M. P. and I. M. Robionson. 1961. An improved nonselective culture medium for ruminal bacteria and its use in determinig diurnal variation in numbers of bacteria in the rumen. J. Dairy Sci. 44, 1446-1456. https://doi.org/10.3168/jds.S0022-0302(61)89906-2
  6. Chaney, A. L. and E. P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8, 130-132.
  7. Dehority, B. A. 1965. Degradation and utilization of isolated hemicellulose by pure cultures of cellulolytic rumen bacteria. J. Bacteriol. 89, 1515-1520.
  8. E. J. Kim and M. S. Ahn. 1993. Antioxidative Effect of Ginger Extracts. Kr. J. Soc. Food Sic. 9, 37-42.
  9. Fedorak, P. M. and S. E. Hrwdey. 1983. A simple apparatus for measuring gas production by methanogenic cultures in serum bottles. Environ. Technol. Lett. 4, 425-432. https://doi.org/10.1080/09593338309384228
  10. Ha, Y. K., J. M. Storkson and M. W. Pariza. 1990. Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasis by conjugated dienoic derivatives of linoleic acid. Cancer Res. 50, 1097-1101.
  11. Ha, Y. L., N. K. Geimm and M. W. Pariza. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcino Genesis 8, 1881-1887. https://doi.org/10.1093/carcin/8.12.1881
  12. Hoffman, P. S., L. Pine, and S. Bell. 1983. Production of superoxide and hydrogen peroxide in medium used to culture leginonella pneymophila : catalystic decomposition by charcoal. Appl. Environ Microbial. 45, 784-791.
  13. Holdman, L. V., E. P. Cato and W. E. C. Moore. 1977. Anaerobic laboratory manual (4th ed.), Virginia Polutech. Inst. And Sate. Univ. Blackburg, Virginia. USA.
  14. Hungate, R. E. 1966. The rumen and it's microbes. pp. 92-446, Academic Press, NY. USA.
  15. Hungate, R. E. 1968. Ruminal fermentation. Hand book of physiology. Allimentary canal. V, 2725-2745.
  16. J. H. Kang, B. W. Ahn, D. H. Lee, H. S. Byun, S. B. Kim and Y. H. Park. 1988. Inhibitory Effects of Ginger and Garlic Extracts on the DNA Damage. Kr. J. Food SCI. Technol. 20, 287-292.
  17. J. K. Ha, S. S. Lee, Y. S. Moon, C. H. Kim. 2005. Ruminant Nutrition and Physiology. pp. 122-239, Seoul National University Press. Seoul, Korea.
  18. J. K. HA. 2001. Study on the Development of Low Pollution Swine Diets and Improving Survival Rate of New Born Piglets. pp. 1-156, Korea Department Ministry of Agriculture and Forestry.
  19. K. S. Yang, J. H. Yu, J. I. Hwang, R. Yang. 1974. Synergistic Effect of Citric Acid on Antioxidant Property of Red Pepper. Kr. J. Food SCI. Technol. 6, 193-198.
  20. Kakano, K., H. Kataoka and M. Matsumura. 1996. High density culture of Propionic-bacterium freudenreichii coupled with propionic acid removal system with activated charcoal. J. Ferment and Boieng. 81, 37-41. https://doi.org/10.1016/0922-338X(96)83117-5
  21. Lee, Y. B., Y. S. Kim and C. R. Ashmore. 1986. Antioxidant property in ginger Rhizome and its application to meat product. Food Sci. 51, 20-23. https://doi.org/10.1111/j.1365-2621.1986.tb10826.x
  22. Lowe, S. E., M. K. Theodorou, A. P. J. Trinci and R. B. Hespell. 1985. Growth of an anaerobic rumen fungi on defind and semi-defind media lacking rumen fluid. J. Gen. Microbiol. 131, 2225-229.
  23. M. L. Kim. 2002. Function of Spice and Herbs. J. East Asian Soc. Dietary Life 12, 431-453.
  24. Miller, G. L., R. Blum, W. E. Glennon and A. L. Burton. 1960. Measurement of carboxylmethylcellulase activity. Anal. Biochem. 23, 257-270.
  25. Morries, J. G., R. L. Baldwin, W. J. Maeng and B. T. Maeda. 1975. Basic characteristics of a computer simulated model of nitrogen in the grazing ruminent. Tracer studies on non-protein nitrogen for ruminant. II. International Atomic Energy Agency. Vienna. Italy.
  26. Park, Y., J. M. Storkson, K. J. Albright, W. Liu and M. W. Pariza. 1999. Evidence that the trans-10,cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids 34, 235-241. https://doi.org/10.1007/s11745-999-0358-8
  27. Parodi, P. W. 1994. Conjugated linoleic acid: An anticarcinogenic fatty acid present in milk fat. Aust. J. Dairy Technol. 49, 93-97.
  28. S. S. Lee, J. K. Ha, Y. J. Choi, In K. Han, T. H. Moon and C. H. Kim. 1995. Studies on the Isolation and Identification of Rumen Fungi Characterization of Cellulolytic Fungal Enzymes, and Its Industrial Utilization. II. Effects of Chemical Treatment of Rice Straw and Animal Species Fed Rice Straw and Animal Species Fed Rice Straw on the Anaerobic Fungal Population in the Rumen. Kor. J. Anim. Nutri. Feed 19, 411-418.
  29. SAS. 1996. SAS Uaer Guide. Release 6.12 edition. SAS Inst. Inc. Cary NC. USA.
  30. Scimeca, J. A. 1998. Toxicological evaluation of dietary conjugated linoleic acid in male Fisher 344 rats. Food Chem. Toxicol. 36, 391-395 https://doi.org/10.1016/S0278-6915(97)00169-5
  31. W. D. Ji, H. C. Chung, M. S. Jeong, S. J. Lee and Y. G. Chung. 1997. Antimicrobial Activity and Distilled Components of Garlic(Allium sativum L .) and Ginger (Zingiber officinale Roscoe). J. Korean Soc. Appl. Biol. Chem. 40, 514-518.

피인용 문헌

  1. Effect of Herbal Extracts Supplementation on Ruminal Methane Production and Fermentation Characteristics In vitro vol.21, pp.9, 2011, https://doi.org/10.5352/JLS.2011.21.9.1315
  2. Effects of Herbal Medicine By-products on Rumen Fermentation Characteristics In vitro vol.48, pp.2, 2014, https://doi.org/10.14397/jals.2014.48.2.89