생명과학회지 (Journal of Life Science)

  • 제17권10호
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  • Pages.1434-1440
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  • 2007
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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경구투여에 의한 버섯균사체 배양액이 넙치의 성장 및 비특이적 면역활성에 미치는 영향

Effect of Culture Broth from Mushroom Mycelium on Growth and Non-specific Immune Parameters in Flounder (Paralichthys olivaceus) by Oral Administration

  • 김만철 (제주대학교 해양과학대학 수산생명의학과) ;
  • 김민주 (제주대학교 해양과학대학 수산생명의학과) ;
  • 김주상 (제주대학교 해양과학대학 수산생명의학과) ;
  • 허문수 (제주대학교 해양과학대학 수산생명의학과)
  • Kim, Man-Chul (Department of Aquatic Life Medicine, Cheju National University) ;
  • Kim, Min-Joo (Department of Aquatic Life Medicine, Cheju National University) ;
  • Kim, Ju-Sang (Department of Aquatic Life Medicine, Cheju National University) ;
  • Heo, Moon-Soo (Department of Aquatic Life Medicine, Cheju National University)
  • 발행 : 2007.10.30
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초록

본 연구는 넙치의 면역반응에서 버섯균사체 (상황버섯, 운지버섯) 혼합배양추출물의 효과에 대해서 연구하였다. 혼합배양추출물은 넙치에 대해서 성장, 혈액, 라이소자임활성, 백혈구, 식세포활성 및 Vibrio anguillarum에 대한 질병저항성에 관해서 평가되었다. 성장에 있어서 버섯균사체 혼합 배양 추출물을 섭이한 그룹의 체중은 52 g 전장은 2.1 cm가 대조구보다도 증가하였다. 혈액학적으로 버섯균사체 혼합 배양추출물을 경구투여 했을 때 글루코오스의 양이 증가하였다. 그러나 각 그룹에 대한 COT (glutamic oxaloacetic transaminase), GPT (glutamlc pyruvic transamlnase), TG, TP, LDH (lactate dehydronase)의 유의적인 차이는 없었다. 라이소자임 활성은 대조구 보다 실험구에서 80%의 높은 결과를 얻을 수 있었으며, 식세포 활성은 실험구에서 대조구 보다 60% 높은 활성을 보였다. 라이소자임 활성과 식세포 활성의 결과 경우에는 12주후에 다소 감소하는 양상을 보였지만, 대조구 보다는 높은 활성을 보였다. 그리고 $7{\times}10^8\;CFU$ 농도로 어류 병원성 세균인 Vibrio anguillarum를 어류 당 투여하여 병원성 테스트를 한 결과 누적 폐사율을 대조구와 비교해 보았을 때 25%의 높은 생존율을 보였다.

We have investigated the effects of mushroom mycelium (Phellinus linteus, Coriolus versicolor) mixed cultural extract on the immune responses of olive flounder, Paralichthys olivaceus. The mixed culture extracts were evaluated for the growth, hematology, lysozyme activity, leukocyte phagocytic activity, and disease resistance against Vibrio anguillarum. In the effect of the growth, the body weight and length gain in the group, which fed with mushroom mycelium mixed cultural extract, were 52 g and 3.4 cm higher than that in the control, respectively. For the hematology, the administration of mushroom mycelium mixed cultural extract resulted in increase of glucose. However, there was no distinct differences in GOT (glutamic oxaloacetic transaminase), GPT (glutamlc pyruvic transamlnase), TG, TP, and LDH (lactate dehydronase) among each group. The activities of lysozyme were 80% higher in the experimental groups than in the control. The activities of leucocyte were 66% higher in the experimental groups than in the control. Although lysozyme activity and leucocyte activity showed somewhat decrease after 12 weeks, these activities were still higher than in the control. The cumulative mortality (%) after an artificial challenge with $7{\times}10^8\;CFU$ of Vibrio anguillarum per fish was 25% higher in the experimental groups than the control.

키워드

참고문헌

  1. Anderson, D. P. 1992. Immunostimumulants, adjuvants, and vaccine carrires in fish: applications to aquaculture. Annu. Rev. Fish Dis. 2, 281-307. https://doi.org/10.1016/0959-8030(92)90067-8
  2. Baulny, M. O., C. Quentel, V. Foundier, F. Lamour and R. L. Gouvello. 1996. Effect of long term oral administration of $\beta$-glucan as an immunomodulant or an adjuvant on some non-specific parameters of the immune response of turbot scophthalmus maximus. Dis. Aquat. Org. 26, 139-147. https://doi.org/10.3354/dao026139
  3. Duncan, P. L. and P. H. Klesius. 1996. Dietary immunomodulants enhance nonspecifis immune responses in channel catifish but not resistance to Edwardsiella ictaluri. T. Aquat. Anim. Health 8, 241-248. https://doi.org/10.1577/1548-8667(1996)008<0241:DIENIR>2.3.CO;2
  4. Ebihara, K. and Y. Minamishima. 1984. Protective effect of biological response modifiers on murine cytomegalovirus infection. J. Virology 51, 117-120.
  5. Ellis, A. E. 1999. Immunity to bacteria in fish. Fish & Shellfish Immunol. 9, 291-308. https://doi.org/10.1006/fsim.1998.0192
  6. Figueras, A., M. M. Santarem and B. Novoa. 1997. In vitro immunostimulation of turbot (Scophthalmus maximus) leukocytes with $\beta$-glucan and/or Photobacterium damsela bacterin. Fish Pathol. 32, 153-157. https://doi.org/10.3147/jsfp.32.153
  7. Hikino, H., C. Kanno. Y. Mirin and T. Hayashi. 1985. Isolation and hypoglycemic activity of Ganoderans A and B, glycans of Ganoderman lucidum fruit bodies. Planta. Med. 51, 339-340. https://doi.org/10.1055/s-2007-969507
  8. Ikekawa, T., M. Nakanishi, N. Uehara, G. Chihara and F. Fukuoka. 1969. Antitumor activitn of aqueous extracts of edible mushroom. Jpn. J. Cancer Res. 29, 734-735.
  9. Jolles, P. and J. Jolles. 1984. What is new in lysozyme research Always a model system, todays as yesterday. Mol. biachem. 63, 165-189.
  10. Jung, I. C., S. Park, K. S. Park and C. H. Ha. 1996. Antioxidative effect of fruit body and mycelia extracts of Pleurotus ostreatus. Korean J. Food Sci. Technol. 28, 464-469.
  11. Kim, B. K., S. Y. Jang and M. J. Shim. 1978. Studies on the higher fungi of korea (VIII), Sterols of coriolus versicolor (Fr), Korean J. Mycology 6, 1-4.
  12. Kim, J. W., S. I. Park and S. K. Chun. 1992 Purification and antibacterial effect of lysozyme from flounder, Paralichthys olivaceus. J. Fish Pathol. 5, 87-92.
  13. Kupka, J., T. Anke and F. Oberwinkler, 1979. Antibiotics from Basidiomycetes. VII. Crinipellin, A New Antibiotic from the Basidiomycetous Fungus Crinipellis stipitaria (Fr.) Pat. J. Antibiotics 32(2), 130-135. https://doi.org/10.7164/antibiotics.32.130
  14. Mau, J. T., H. C. Lin and C. C. Chen. 2001. Non-volatile components of several medicinal mushrooms. Food Research International 3, 521-526.
  15. Park, S. W., J. K. Kwak, J. G. Koo and M. G. Cho. 2001. Effects of $\beta$-glucan from Schizophillum commune on Nonspecific immune parameters in common carp (Cyprinus carpio) and Flounder (Paralichthys olivaceus) by oral administration. J. Korean Fish Soc. 34(4), 412-418.
  16. Park, S. W., Y. G. Kim and D. L. Choi. 1996. Increase in phagocytic activity of peripheral neutrophil and lysozyme activity of blood serum in Korea catfish (Silurus asotus) intraperitoneally injected with $\beta$-glucan. J. Fish Pathol. 9, 87-93.
  17. Robertsen, B., R. E. Engstad and J. B. Jorgensen. 1994. $\beta$- glucans as immunostimulants in fish. pp. 83-99, In: Stolen, J. S., Fletcher, T. C. (Eds.), Modulators of Fish Immune Responses, vol. 1. SOS Publications, Fair Haven, NJ.
  18. Song, C. H., J. H. Kim, B. K. Yang and K. W. Kim. 1996. Anti-complementary polysaccharides produced from submerged mycelial culture of Pleurotus sajocaju. Korea J. Mycology 24, 104-110.
  19. Suzuki, S. and S. Oshima. 1976. Influence of Shitake (Lentinus edodes) on human serum cholesterol. Mushroom Sci. 9, 463-467.
  20. Tsukagoshi, S. and F. Ohashi. 1974. Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma 180 and rat ascites hepatoma AII-13 by oral use. Jpn. J. Cancer Res. 65, 557-560.
  21. Williams, D. L., I. W. Browder and N. R. Luzio. 1983. Immunotherapeutic modification of Escherichia coli-induced experimental peritonitis and bacteremia by glucan. Surgery 93, 448-454.
  22. Wedemeyer, G. A. and W. T. Yasutake. 1977. Clinical methods for the effects of environmental stress on fish health. U.S. pp. 89, Fish and Wildlife Service Technical.
  23. Won, K. M., S. M. Kim and S. I. Park. 2004. The Effects of $\beta$-1,3/1,6-linked Glucan in the Diet on Immune Responses of Olive Flounder, Paralichthys olivaceus by Oral Administration. J. Fish Pathol. 17(1), 29-38.
  24. Yoshida, T., R. Kruger and V. lnglis. 1995. Aguroentation of non-specific in African catfish, Clarias gariepinus (Burchell), by the long-term oral administration of immunostimulants. J. Fish Dis. 18, 195-198. https://doi.org/10.1111/j.1365-2761.1995.tb00278.x

피인용 문헌

  1. Application of phytochemicals as immunostimulant, antipathogenic and antistress agents in finfish culture vol.3, pp.3, 2011, https://doi.org/10.1111/j.1753-5131.2011.01048.x