Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effect of Pimpinella Brachycarpa Ethanol Extract on Chronically Ethanol-Induced Liver Damage in Rats

참나물이 만성적으로 알코올 유도된 흰쥐의 간손상에 미치는 영향

  • 추명희 (조선대학교 식품영양학과) ;
  • 이재준 (조선대학교 식품영양학과) ;
  • 이명렬 (조선대학교 식품영양학과)
  • Published : 2007.10.30
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Abstract

This study was performed to investigate the effect of ethanol extract of Pimpinella brachycarpa(PBE) on chronically ethanol-induced hepatotoxicity in rat liver. Sprague-Dawley rats weighing 90-130 g were divided into 5 groups; normal group(NOR), ethanol(35%, 10 ml/kg) treated group(CON), PBE 200 mg/kg treated group(P1), PBE 200 mg/kg and ethanol treated group(P2), and PBE 400 mg/kg and ethanol treated group(P3). PBE was also fractionated by the following solvent: n-hexane, chloroform, ethylacetate and n-butanol. The antioxidative capacity of the n-hexane fraction was the highest among fractions and was similar to that of butylated hydroxytoluene(BHT). The body weight gain and feed intake of the rats were decreased by ethanol administration, but were gradually increased to the similar levels of the NOR group by administering PBE. The AST activity in serum elevated by ethanol was significantly decreased by administering the high dosage of PBE, but exerted no significant change on serum ALT activity. It was also observed that the hepatic activities of xanthine oxide(XO), catalase and glutathione peroxidase(GSH-Px) increased by ethanol were markedly decreased in the combined ethanol and PBE administered groups(P2 and P3), but not in the activity of superoxide dismutase(SOD) as compared with the CON group. The glutathione(GSH) contents were decreased by ethanol adminstration, however, increased after administering PBE. These results suggest that ethanol extract of Pimpinella brachycarpa has a possible positive effect on the liver function in hepatotoxicity-induced rats by ethanol administration.

본 연구에서는 참나물의 항산화효과를 구명하기 위하여, in vitro에서 Rancimat에 의한 항산화력 측정과 in vivo에서 Sprague Dawley 종 수컷에게 만성적으로 알코올 과량 섭취로 유도된 간조직의 산화적 스트레스가 참나물을 처리함으로써 간조직의 항산화 체계에 어떠한 영향을 미치는지를 비교 관찰하여 다음과 같은 결과를 얻었다. 실험군은 정상군(NOR), 대조군(CON, 35% 알코올 10 ml/kg of b.w./day), 참나물 에탄올 추출물 단독투여군(P1), 알코올과 참나물 에탄올 추출물 저용량(200 mg/kg of b.w./day) 병합투여군(P2) 및 알코올과 참나물 에탄올 추출물 고용량(400 mg/kg of b.w./day) 병합투여군(P2)의 5군으로 나누어 6주간 사육하였다. In vitro 연구 결과는 참나물 에탄올 추출물을 계통 분획하여 Rancimat에 의한 항산화지수를 측정하였는데, 분획 중 n-hexane의 항산화력이 가장 우수하였으며, 합성항산화제인 BHT와 항산화력이 비슷하였다. In vivo에서 항산화 효과를 측정한 결과는 정상식이만을 급여한 정상군과 정상식이와 참나물 에탄올 추출물을 단독투여 한 P1군간에는 유의차가 없었으나, 알코올을 투여한 군(대조군, P2 및 P3)들 간에는 유의차를 보였으며 특히, 고용량 병합투여 시 항산화 효과가 높게 나타났다. 즉 만성 알코올 투여로 감소되어진 체중과 식이섭취량은 참나물 에탄올 추출물 병합투여로 정상식이군 수준으로 회복시켰다. 또한 참나물 에탄올 추출물 병합투여 시 알코올 투여로 증가된 간조직의 유리기 해독계 효소인 XO, catalase 및 GSH-Px 활성 억제와 항산화작용을 나타내는 GSH 함량을 증가시킴으로서 지질과산화물에 대한 방어력이 증가되었다. 이상의 결과 알코올과 참나물 에탄올 추출물을 병행 투여함으로서 항산화 효소 체계가 향상되었으며, 참나물 에탄올 추출물은 알코올 섭취로 유도된 산화적 스트레스와 알코올성 독성으로부터 간을 보호하는 항산화효과가 있는 것으로 추정된다.

Keywords

References

  1. Aebi, H. 1984. Catalase in vitro. Method Enzymol. 105, 121-126. https://doi.org/10.1016/S0076-6879(84)05016-3
  2. Akaike, T., S. Ijiri, K. Sato, T. Katsuki and H. Maeda. 1995. Determination of peroxyl radical-scavenging activity in food by using bacterial action of alkyl peroxyl radical. J. Agri. Food Chem. 43, 1864-1870. https://doi.org/10.1021/jf00055a021
  3. Aykac, G., M. Uysal., A. S. Yalcin, N. Kocak-Toker, A. Sivas and H. Oz. 1985. The effects of chronic ethanol ingestion on hepatic lipid peroxide, glutathione peroxide, and glutathione transferase in rats. Toxicol. 36, 71-76. https://doi.org/10.1016/0300-483X(85)90008-3
  4. Bompart, G. J., D. S. Prevot and J. L. Bascabds. 1990. Rapid automated analysis of glutathione reductase, peroxidase and S-transferase activity: application to cisplatin-induced toxicity. Clin. Biochem. 23, 501-504. https://doi.org/10.1016/0009-9120(90)80039-L
  5. Buege, J. A. and S. D. Aust. 1978. The thiobarbituric acid assay. Methods in Enzymology 5, 306-307.
  6. Cha, G. S. and C. U. Choi. 1990. Determination of oxidation stability of perilla oil by the Rancimat method. Korean J. Food Sci. Technol. 22, 61-65.
  7. Cho, S. H., J. C. Kim and S. W. Kim. 2001. Effect of plant extracts on the activity of alcohol dehydrogenase and the antioxidant in alcohol-treated rat hepatocyte. J. Korean Soc. Food Sci. Nutr. 30, 679-683.
  8. Cho, S. Y., M. K. Lee, E. M. Park, J. Y. Jang and M. J. Kim. 1997. Effect of methionine levels on brain lipid peroxidation in ethanol-treated rats of selenium deficiency. J. Korean Soc. Food Sci. Nutr. 26, 109-115.
  9. Crapo, J. D., J. M. McCord and I. Fridovich. 1978. Preparation and assay of superoxide dismutase. Methods enzymol. ed. Fleischer, S. and Packer, L. Academic press., New York, 53. 382-393.
  10. Dolphin, D., A. Forman, D. C. Borg, J. Fajer and R. H. Felton. 1971. Compounds I of catalase and horeradish peroxidase. Proc. Natl. Acad. Sci. USA 68, 614-618. https://doi.org/10.1073/pnas.68.3.614
  11. Downey, J. M., Y. Miura, L. J. Eddy, D. E. Chambers, T. Mellert, D. J. Hearse and D. M. Yellon. 1987. Xanthine oxidase is not a source of free radicals in the ischemic rabbit heart. J. Mol. Cell Cardiol. 19. 1053-1060. https://doi.org/10.1016/S0022-2828(87)80350-4
  12. Duke, E. J., P. Joyce and J. P. Ryan. 1973. Characterization of alternative molecular forms of xanthine oxidase in the mouse. Biochem. J. 131, 187-190. https://doi.org/10.1042/bj1310187
  13. Esquivel, M. M., M. A. Ribeiro and M. G. BermardoGil. 1999. Supercritical extraction of savory oil: study of antioxidant activity and extract characterization. J. Supercritical Fluids 14, 129-138. https://doi.org/10.1016/S0896-8446(98)00115-6
  14. Ferrali, M., L. Ciccoli and M. Comporti. 1989. Ally alcohol induced hemolysis and its relation to iron release and lipid peroxidation. Biochem. Pharmacol. 38, 1819-1825. https://doi.org/10.1016/0006-2952(89)90417-6
  15. Flohe, L., A. Wolfgang and W. A. Gunzler. 1984. Assay of glutathione peroxidase. Methods in enzymatic analysis. Packer, L, eds., Academic Press., New York, 105, 114-130.
  16. Fritche, K. and P. V. Johnston. 1988. Rapid autooxidation of fish oil in diets without added antioxidants. J. Nutr. 118, 425-426. https://doi.org/10.1093/jn/118.4.425
  17. Fujji, H., T. Ohmachi, I. Sagami and M. Watanabe. 1985 Liver microsomal drug metabolism in ethanol-treated hamsters. Biochem. Pharmacol. 34, 3881-3884. https://doi.org/10.1016/0006-2952(85)90438-1
  18. Girotti, A. W., J. P. Thomas and J. E. Jordan. 1985. Inhibitory effect of Zinc(II) on free radical lipid peroxidation in erythrocyte membranes. J. Free Radic. Biol. Med. 1, 395-401. https://doi.org/10.1016/0748-5514(85)90152-7
  19. Glueck, C. J., E. Hogg, C. Allen and P. S. Gartside. 1980. Effects of alcohol ingestion on lipid and lipoprotein in normal man. Am. J. Clin. Nutr. 33, 2287-2293. https://doi.org/10.1093/ajcn/33.11.2287
  20. Halliwell, B. and J. M. C. Gutteridge. 1989. Lipid peroxidation a radical chain reaction. pp. 189-233. In Free radicals in Biology and Medicine, 2nd ed., Oxford University Press., London.
  21. Ham, S. S., S. W. Kim and Y. M. Kim. 1990. Studies on antimutagenic effects and gene repair of enzymatic browning reaction products. Korean J. Food Sci. Technol. 22, 632-639.
  22. Joo, K. L. and J. J. Kim. 2002. Oxidative stability and compounds of sesame oils blended with vegetable oils. Korean J. Food Sci. Technol. 34, 499-502.
  23. Jung, G. T., I. O. Ju, J. S. Choi and J. S. Hong. 2000. The antioxidative, antimicrobial and nitrite scavenging effects of Schizandra chinensis RUPRECHT(Omija) seed. Korean J. Food Sci. Technol. 32, 928-935.
  24. Keshavarzian, A., J. Z. Fields, J. Vaeth and E. W. Holmes. 1994. The differing effects of acute and chronic alcoholic on gastric and intestinal permeability. Am. J. Gastro. 8, 2205-2212.
  25. Kim, J. D., S. Y. Lee and S. W. Kim. 1997. Modulation of hepatic lipid peroxidation and antioxidant defenses by wild plants extracts. Kor. J. Pharmacogn. 28, 48-53.
  26. Ko, K. S. and E. S. Jeon. 2003. Ferns, fern-allies and seed beating plant of Korea. pp. 481, Iljinsa., Seoul.
  27. Koo, B. K., J. M. Chung and H. S. Lee. 1998. Biochemical evaluation of nutritional status of protein and lipid in patients with alcoholic liver disease. Korean J. Food Sci. Nutr. 27, 1236-1243.
  28. Lee, J. J., M. H. Choo and M. Y. Lee. 2006. Effect of Pimpinella brachycarpa extract on lipid metabolism in rats fed high cholesterol diet. J. Korean Soc. Food Sci. Nutr. 35, 1151-1158. https://doi.org/10.3746/jkfn.2006.35.9.1151
  29. Lee, J. J., M. H. Choo and M. Y. Lee. 2007. Physicochemical compositions of Pimpinella brachycarpa. J. Korean Soc. Food Sci. Nutr. 36, 327-331. https://doi.org/10.3746/jkfn.2007.36.3.327
  30. Lee, S. H. 1998 Studies on antimutagenicity and cytotoxicity of edible mountain herbs extracts. Master's thesis, Kangwon University.
  31. Lieber, C. S. 1991. Hepatic, metabolic and toxic effects of ethanol. Alcohol Clin. Exp. Res. 15, 573-592. https://doi.org/10.1111/j.1530-0277.1991.tb00563.x
  32. Lieber, C. S. 1994. Alcohol and liver. Gastro. 106, 1085-1180. https://doi.org/10.1016/0016-5085(94)90772-2
  33. Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275.
  34. McCord, J. M. and I. Fridovich. 1969. Superoxide dismutase: An enzymatic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244, 6049-6055.
  35. Mezey, E. 1980. Alcoholics liver disease: roles of alcohol and malnutrition. Am. J. Clin. Nutr. 33, 2709-2718. https://doi.org/10.1093/ajcn/33.12.2709
  36. Munday, R and C. C. Winterbourm. 1989. Reduced glutathione in combination with superoxide dismutase as an important biological antioxidant defence mechanism. Biochem. Pharmacol. 38, 4349-4352. https://doi.org/10.1016/0006-2952(89)90641-2
  37. Oh, D., S. S. Ham, S. Y. Lee, B. K. Park, S. H. Kim, C. K. Chung and I. J. Kang. 1998. Effect of irradiation and blanching on the quality of juices of Spuriopinella brachycarpa during storage. Korean J. Food Sci. Technol. 30, 333-340.
  38. Oh, S. I., C. I. Kim and S. C. Park. 1998. Chronic ethanol consumption affects glutathiones status in rat liver. J. Nutr. 128, 758-763. https://doi.org/10.1093/jn/128.4.758
  39. Okeefe, J. H., C. J. Lavie and B. D. McCallister. 1995. Insights into the pathogenesis and prevention of coronary artery disease. Mayo Clin. Proc. 70, 69-79. https://doi.org/10.4065/70.1.69
  40. Plaa, G. L. and H. Witscri. 1976. Chemicals, drugs and lipid peroxidations. Annu. Rev. Pharmacol. Toxicol. 16, 125-141. https://doi.org/10.1146/annurev.pa.16.040176.001013
  41. Prasad, K. and J. Kalra. 1993. Oxygen free radicals and hypercholesterolemic atherosclerosis: effect of vitamin E. Am. Heart J. 125, 958-973. https://doi.org/10.1016/0002-8703(93)90102-F
  42. Reeves, P. G., F. H. Nielson and G. C. Fahey Jr. 1993. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 123, 1939-1951. https://doi.org/10.1093/jn/123.11.1939
  43. Reitman, S. and S. Frankel. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic determination and glutamic pyruvic transaminase. Am. J. Clin. Pathol. 28, 56-63. https://doi.org/10.1093/ajcp/28.1.56
  44. Rya, S. Y. and Kim. J. H. 1995. Effect of chronic alcohol feeding and 2-acetylaminofluorence treatment on hepatic mitochondrial ATPase activity and membrane lipid composition in rats. J. Korean Soc. Food Nutr. 24, 867-873.
  45. Shaw, S. and C. S. Lieber. 1983. Nutrition and alcohol, A clicnical perpective, In : Weininger J. Briggs GM, eds. Nutrition Update John Wiley & Sons, New York, 1, 79-104.
  46. Speisky, H., Y. Kera, K. E. Penttila, Y. Israel and K. O. Lindros. 1988. Depletion of hepatic glutathione by ethanol occurs independently of ethanol metabolism. Alcohol Clin. Exp. Res. 12, 224-228. https://doi.org/10.1111/j.1530-0277.1988.tb00184.x
  47. Sun, B. Y. 2002. The palnts of the Umbelliferae family distributed in Korea. Korean J. Pl. Taxon. 30, 93-104.
  48. Tietze, F. 1969. Enzymatic methods for quantitative determination of nanogram amounts of total and oxidized glutathione. Anal. Biochem. 27, 502-522. https://doi.org/10.1016/0003-2697(69)90064-5
  49. Vina, J., J. M. Estrella, C. Guerro and F. J. Romero. 1980. Effects of ethanol on glutathione concenturation in isolated hepatocytes. Biochem. J. 188, 549-552. https://doi.org/10.1042/bj1880549

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