생명과학회지 (Journal of Life Science)

  • 제17권10호
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  • Pages.1374-1380
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  • 2007
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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유전자 검색법을 이용한 상수원수와 정수처리 공정중의 병원성 미생물 검출 및 분포특성

Detection and Distribution of Bacterial Pathogens in Raw Water and During Water Treatment Process by Polymerase Chain Reaction

  • 박홍기 (부산광역시 상수도사업본부 수질연구소) ;
  • 정은영 (부산광역시 상수도사업본부 수질연구소) ;
  • 정종문 (부산광역시 상수도사업본부 수질연구소) ;
  • 유평종 (부산광역시 상수도사업본부 수질연구소)
  • Park, Hong-Ki (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Jung, Eun-Young (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Jung, Jong-Moon (Water Quality Institute, Water Works HQ of Busan Metropolitan City) ;
  • Yu, Pyung-Jong (Water Quality Institute, Water Works HQ of Busan Metropolitan City)
  • 발행 : 2007.10.30
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초록

유전자 검색법(PCR)은 환경시료중의 병원성 미생물을 검출하는데 있어서 전통적인 배양법이 가지고 있는 문제점을 보완할 수 있는 장점을 지니고 있다. 이러한 PCR을 이용하여 부산시 취수원과 정수공정별 지점을 대상으로 병원성 미생물의 검출과 분포특성에 대해 조사하였다. 2004년 8월에서 2005년 10월까지의 실험 결과에 의하면 상수원수의 92개 시료중 80개 시료에서(87.0%) 병원성 미생물이 검출되었고, Shigella spp. 46.2%, Yersinia spp. 40.7%, Legionella spp. 17.6% 그리고 Salmonella spp. 9.9%의 검출율을 각각 보였다. 원수중의 병원성 미생물은 늦은 가을에서 겨울에 걸쳐 주로 분포하는 특징을 보였으며, 지점별로는 매리 원수가 물금 원수보다 높게 검출되었다. 정수처리 공정별 실험에서는 Shigella의 검출빈도가 높았으나 모든 병원성 미생물은 후오존 처리 공정 단계에서 완전히 제거되는 것으로 나타났다. 따라서 부산시 정수장은 병원성 미생물에 대해 안전함을 알 수 있었다.

The development of polymerase chain reaction (PCR) technology has the potential to solve for isolating pathogenic microorganisms from environmental samples than traditional plate counting methods. We have been detected pathogenic bacteria from raw water and water treatment process in Busan metropolitan city by PCR method. According to the result of survey from July 2004 to October 2005, 80 out of 92(87.0%) were positive for bacterial pathogens in raw water samples and positive rate of Shigella spp., Yersinia spp., Salmonella spp. and Legionella spp. were 46.2%, 40.7%, 17.6% and 9.9%, respectively. Pathogenic bacteria in raw water was mainly distributed through the lately Autumn to the winter and more highly detected Maeri than Mulgum region. During the period of survey in water treatment process, Shigella spp. was highly detected but all of bacterial pathogens were entirely removed after in post-ozonation step. These suggest that waters supplied in Busan metropolitan city may be safe against the pathogenic bacteria.

키워드

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  2. Analysis of Waterborne Pathogenic Bacteria among Total Coliform Positive Samples in the Groundwater of Chungcheongnam-do Province, Korea vol.42, pp.3, 2016, https://doi.org/10.5668/JEHS.2016.42.3.189
  3. Analysis of Bacterial Diversity in Water from the Han River Water Source Protection Area via a Pyrosequencing Assay vol.42, pp.4, 2016, https://doi.org/10.5668/JEHS.2016.42.4.274