Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Antioxidative Properties of Extract/Fractions of Suaeda asparagoides and Salicornia herbacea Extracts(I)

함초(나문재 및 퉁퉁마디) 추출물의 항산화 작용에 관한 연구(I)

  • Park, Soo-Nam (Department of Fine Chemistry, College of Nature & Life Science, Seoul National University of Technology) ;
  • Jeon, So-Mi (Department of Fine Chemistry, College of Nature & Life Science, Seoul National University of Technology) ;
  • Kim, So-I (Department of Fine Chemistry, College of Nature & Life Science, Seoul National University of Technology) ;
  • Ahn, Jeung-Youb (Saeng Green Cosmetics Co., Ltd)
  • 박수남 (서울산업대학교 자연생명과학대학 정밀화학과) ;
  • 전소미 (서울산업대학교 자연생명과학대학 정밀화학과) ;
  • 김소이 (서울산업대학교 자연생명과학대학 정밀화학과) ;
  • 안정엽 ((주)생그린)
  • Published : 2007.09.30
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Abstract

In this study, the antioxidative effects of Sueada asparagoides and Salicornia herbacea extracts were investigated. The free radical(1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity($FSC_{50}$) of extract/fractions of Sueada asparagoides was in the order: 100 % ethanol extract(329.33 ${\mu}g/mL$) < 50 % ethanol extract(40.73) < ethylacetate fraction(13.87) < deglycosylated aglycone fraction (7.80). In case of Salicornia herbacea, the free radical scavenging activities of ethylacetate fraction and aglycone fraction were 13.87 and 7.80 ${\mu}g/mL$, respectively. Reactive oxygen species(ROS) scavenging activities($OSC_{50}$) of Sueada asparagoides and Solicornia herbacea extracts on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The order of ROS scavenging activity of Sueada asparagoides extracts was 50 % ethanol extract($OSC_{50}$, $0.99{\mu}g/mL$) < ethylacetate fraction (0.05) < aglycone fraction (0.03). Aglycone fraction showed the most prominent scavenging activity. In case of Salicornia herbacea, the ROS scavenging activities of ethylacetate fraction and aglycone fraction were 0.10 and 0.20 ${\mu}g/mL$, respectively. The protective effects of extract/fractions of Sueada asparagoides and Salicornia herbacea on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The ethanol extract(100%) of Sueada asparagoides diminished photohemolysis in a concentration dependent manner($1{\sim}100{\mu}g/mL$). Particularly deglycosylated aglycone fraction exhibited the most prominent celluar protective effect($\tau_{50}$, 310 min at 50 ${\mu}g/mL$). In case of Salicornia herbacea, ethylacetate fraction exhibited more potent protective effect. These results indicate that extract/fractions of Sueada asparagoides can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS.

본 연구에서는 함초 추출물(나문재 및 퉁퉁마디)의 항산화능을 조사하였다. 나문재 추출물의 free radical(1,1-diphenyl-2-picrylhyazyl, DPPH) 소거활성($FSC_{50}$)은 100 % ethanol 추출물(329.33 ${\mu}g/mL$) < 50% ethanol 추출물(40.73) < ethylacetate 분획(13.87) < ethylacetate 분획에서 당을 제거시킨 aglycone 분획(7.80) 순으로 증가하였다. 퉁퉁마디의 경우, free radical 소거활성은 ethylacetate 분획 및 aglycone 분획이 각각 23.21 및 28.50 ${\mu}g/mL$이었다. Luminol-의존성 화학발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 나문재 추출물의 총항산화능은 50 % ethanol 추출물($OSC_{50}$, $0.99{\mu}g/mL$) < ethylacetate 분획(0.05) < aglycone 분획(0.03)순으로, aglycone 분획에서 가장 큰 활성을 나타내었다. 퉁퉁마디의 경우, ethylacetate 분획 및 당을 제거시킨 aglycone 분획의 ROS 소거활성은 각각 0.10 및 0.20 ${\mu}g/mL$이었다. 나문재 및 퉁퉁마디 추출물에 대하여 rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 억제 효과를 측정하였다. 100 % ethanol 나문재 추출물의 경우 농도 의존적($1{\sim}100{\mu}g/mL$)으로 광용혈을 억제하였다. 특히 ethylacetate 분획에서 당을 제거시킨 aglycone 분획은 50${\mu}g/mL$ 농도에서 $\tau_{50}$이 310 min으로 매우 큰 세포보호 효과를 나타내었다. 퉁퉁마디의 경우는 ethylacetate 분획에서 비교적 큰 세포보호 활성을 나타내었다. 이상의 결과들은 나문재 추출물이 $^1O_2$ 혹은 다른 ROS를 소광시키거나 소거함으로써 그리고 ROS에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로서 작용할 수 있음을 가리킨다.

Keywords

References

  1. J. C. Fantone and P. A. Ward, Role of oxygenderived free radicals and metabolites in leukocyte dependent inflammatory reaction, Am J. Path, 107, 397 (1982)
  2. K. J. A. Davies, Protein damage and degradation by oxygen radical, J. Biol. Chem, 262, 9895 (1987)
  3. C. S. Foote, Photosensitized oxidation and singlet oxygen; consequences in biological systems, ed. W. A. Pryor, 2, 85, Acdemic press, New York (1976)
  4. S. N. Park, Ph. D. Dissertation, Seoul National Univ., Seoul, Korea (1989)
  5. S. N. Park, Skin aging and antioxidant, J. Soc Cosmet Scientists Korea, 23, 75 (1997)
  6. S. N. Park, Protective effect of isoflavone, genistein from soybean on singlet oxygen induced photohemolysis of human erythrocytes, Korean J. Food Sci. Technol., 35(3), 510 (2003)
  7. S. N. Park, Antioxidative properties of baicalein, component from Scutellaria baicalensis Georgi and its application to cosmetics (1), J. Korean Ind. Eng. Chem, 14(5), 657 (2003)
  8. K. Scharffetter-Kochanek, Photoaging of the connective tissue of skin: its prevention and therapy, antioxidants in disease mechanism and therapy, ed. H. Sies, 38, 639 (1997)
  9. R. M. Tyrrell and M. Pidoux, Singlet oxygen involvement in the inactivation of cultured human fibroblast by UVA and near visible radiations, Photochem Photobiol., 49, 407 (1989) https://doi.org/10.1111/j.1751-1097.1989.tb09187.x
  10. G. F. Vile and R. M. Tyrrell, UV A radiationinduced oxidative damage to lipid and protein in vitro and in human skin fibroblasts is dependent on iron and singlet oxygen, Free Radical Biology & Medicine, 18, 721 (1995) https://doi.org/10.1016/0891-5849(94)00192-M
  11. K. Scharffetter-Kochanek, M. Wlaschek, K. Briviba, and H. Sies, Singlet oxygen induces collagenase expression in human skin fibroblasts, FEBS Lett., 331, 304 (1993) https://doi.org/10.1016/0014-5793(93)80357-Z
  12. M. Wlaschek, K. Briviba, G. P. Stricklin, H. Sies, and K. Scharffetter-Kochanek, Singlet oxygen may mediate the ultraviolet A in induced synthesis of interstitial collagenase, J. Invest. Dermatol., 104, 194 (1995) https://doi.org/10.1111/1523-1747.ep12612751
  13. A. Oikarinen, J. Karvonen, J. Uitto, and M. Hannuksela, Connective tissue alterations in skin exposed to natural and therapeutic UV-radiation, Photodermatology, 2, 15 (1985)
  14. A. Oikarinen and M. Kallioinen, A biochemical and immunohistochemical study of collagen in sunexposed and protected skin, Photodermatology, 6, 24 (1989)
  15. L. H. Kligman, UV A induced biochemical changes in hairless mouse skin collagen: a contrast to UVB effects, ed. F. Urbach, 209, Valdemar, Overland Park (1992)
  16. J. W. Choi, S. I. Kim, J Y. Kim, H. J Yang, K. H. Lee, and S. N. Park, Antioxidative and cellular protective effects of Jeju native plant extracts against reactive oxygen species (1), J. Soc. Cosmet. Scientists Korea, 32(3), 181 (2006)
  17. B. H. Lee, Y. H. Moon, B. C. Jeong, K. S. Kim, and S. N. Ryu, Growth characteristics and it's potentiality of use of halophyte, Suaeda asparagoides MIQ, Korean J. Intl. Agri., 14(2), 87 (2002)
  18. Y. C. Jo, J H. Ahn, S. M. Chon, K. S. Bae, T. J and D. S. Kang, Studies on phannacological effects of glasswort (Salicomia herbacea L.), Korean J. Med. Crop. Sci., 10, 93 (2002)
  19. J. G. Min, K. T. Kim, J. H. Kim, T. J. Kim, and J. H. Park, Physiological and functional properities of Salicomia herbacea leaf extracts, Nutraceut. Food, 7, 261 (2002) https://doi.org/10.3746/jfn.2002.7.3.261
  20. S. H. Park and K. S. Kim, Isolation and identification of antioxidant flavonoids from Salicomia herbacea L, J. Korean Soc. Appl. Biol. Chem, 47(1), 120 (2004)