Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

In Vitro Propagation Through Nodal Explants in Helicteres isora L., a Medicinally Important Plant

  • Shriram, Varsha (Department of Botany, University of Pune) ;
  • Kumar, Vinay (Department of Botany, University of Pune) ;
  • Shitole, M.G. (Department of Botany, University of Pune)
  • Published : 2007.09.29
Download PDF
⟨ Previous Next ⟩

Abstract

Helicteres isora is medicinally important plant effective against asthma, diabetes, hypolipidemia, HIV, besides a good source of diosgenin. Seed dormancy and low rate of natural fruit production make this plant a perfect candidate for developing an in vitro method useful for its clonal propagation and further biotechnological developments. This is the first report on in vitro production of this plant. Nodal explants obtained from aseptically germinated seedlings were cultured on MS medium (Murashige and Skoog 1962) fortified with indole-3-acetic acid (IAA) ($0.57-22.83\;{\mu}M$), indole-3-butyric acid (IBA) ($0.41-16.58\;{\mu}M$), 6-benzylaminopurine (BA) ($0.44-17.75\;{\mu}M$) and kinetin (Kin) ($0.46-13.94\;{\mu}M$) either singly or in combinations of IAA + BA, IAA + Kin and BA + Kin. Combinations of cytokinins (BA and Kin) were most suitable for multiple shoot induction and $13.94\;{\mu}M\;Kin\;+\;13.31\;{\mu}M\;BA$ was optimum (79% frequency) associated with high number of microshoots (7.1 shoots per explant) after 20 days of culture. Maximum shoot elongation and proliferation (10 shoots per explant with 4.8 cm average height) was achieved on MS media containing $2.32\;{\mu}M\;Kin\;+\;2.22\;{\mu}M\;BA\;+\;2.85\;{\mu}M\;IAA$. High rooting frequency (70%) was achieved on MS medium (1/2 basal strength) fortified with $4.14\;{\mu}M$ IBA, while activated charcoal showed inhibitory effects on rooting. Hardening was done with 76% survival rate and these plants were growing without any visual defects and morphologically mimicking the naturally growing plants.

Keywords

References

  1. Al-Khayri JM, Al-Bahrany AM (2001) Micropropagation of Citrus aurantifolia (lime). Curr Sci 81: 1242-1246
  2. Anonymous (1995) Indian medicinal plants: A compendium of 500 species. Orient Longman, Vol. 3: p 132
  3. Atluri JB, Rao PS, Reddi CS (2000) Pollination ecology of Helicteres isora Linn. (Sterculiaceae). Curr Sci 78: 713-719
  4. Badave GN, Jadhav SC (1998) Germination studies in local plants from Koyana Valley, I: Murudsheng- Helicteres isora L. Ayurveda Update 1: 10
  5. Barik B, Dey AK, Das PC (1981) Helicteres isora L., a new source of diosgenin. Indian J Chem 20: 938
  6. Chakrabarti R, Vikramadithyan RK, Mullangi R, Sharma VM, Jagadheshan H, Rao YN, Sairam P, R. Rajagopalan (2002) Antidiabetic and hypolipidemic activity of HeJicteres isora in animal models. J Ethnopharmacol 81: 343-349 https://doi.org/10.1016/S0378-8741(02)00120-4
  7. Joshy MK, Mathew L, Joseph R (2006) Studies on short isora fiber-reinforced polyester composites. Composite Interfaces 13: 377-390 https://doi.org/10.1163/156855406777408566
  8. Kaur K, Kant U (2000) Clonal propagation of Acacia catechu Willd. by shoot tip culture. Plant Growth Reg 31: 143-145 https://doi.org/10.1023/A:1006362318265
  9. Komalavalli N, Rao MV (2000) In vitro micropropagation of Gymnema sylvestre-A multipurpose medicinal plant. Plant Cell Tissue Organ Cult 61: 97-105 https://doi.org/10.1023/A:1006421228598
  10. Kumar G, Murugesan AG, Pandian MR (2006) Effect of Helicteres isora bark extract on blood glucose and hepatic enzymes in experimental diabetes. Pharmazie 61: 353-355
  11. Kusumoto IT, Shimada I, Kakiuchi N, Hattori N, Namba T, Supriyatna S (1992) Inhibitory effects of Indonesian plant extracts of reverse transcriptase of an RNA tumor virusI. Phytother Res 6: 241-244 https://doi.org/10.1002/ptr.2650060504
  12. Maene L, Debergh P (1985) Liquid medium additions to established tissue cultures to improve elongation and rooting in vivo. Plant Cell Tissue Organ Cult 5: 23-33 https://doi.org/10.1007/BF00033566
  13. Makunga NP, Jager AK, Staden JV (2003) Micropropagation of Thapsia garganiccr a medicinal plant. Plant Cell Rep 21: 967-973 https://doi.org/10.1007/s00299-003-0623-8
  14. Martin KP, Beena MR, Joseph O (2003) High frequency axillary bud multiplication and ex vitro rooting of WedeJia chinensis (Osbeck) Merr.- a medicinal plant. Indian J Exp Biol 41: 262-266
  15. Meng-Iing HE, Shu-yuan Q, Lan-juan HU (2005) Rapid in vitro propagation of medicinally important Aquilaria agallocha. J Zhejiang Univ Sci 6: 849-852
  16. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473-479 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  17. Otake T, Mori H, Morimoto M, Ueba N, Sutardjo S, Kusomoto I, Hattori M, Namba T (1995) Screening of Indonesian plant extracts for anti human immuno deficiency virus type 1 (HIV-l) activity. Phytother Res 9: 6-10 https://doi.org/10.1002/ptr.2650090103
  18. Pohocha N, Grampurohit ND (2001) Antispasmodic activity of the fruits of Helicteres isora Linn. Phytother Res 15: 49-52 https://doi.org/10.1002/1099-1573(200102)15:1<49::AID-PTR729>3.0.CO;2-E
  19. Purohit, SD, Dave A (1996) Micropropagation of Sterculia urens Roxb.: An endangered tree species. Plant Cell Rep 15: 704-706 https://doi.org/10.1007/BF00231929
  20. Soniya EV, Das MR (2002) In vitro micropropagation of Piper longum- an important medicinal plant. Plant Cell Tissue Organ Cult 70: 325-327 https://doi.org/10.1023/A:1016561521050
  21. Venkatesh S, Reddy GD, Reddy YS, Sathyavathy D, Reddy BM (2004) Effect of Helicteres isora root extracts on glucose tolerance in glucose-induced hyperglycemic rats. Fitoterapia 75: 364-367 https://doi.org/10.1016/j.fitote.2003.12.025