Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Rapid Propagation through Tissue Culture of Cudrania tricuspidata, Medicinal Plant

  • Lee, Cheul-Ho (Department of Plant Conservation, Korea National Arboretum) ;
  • Min, Ji-Yun (Division of Environmental Forest Science, Gyeongsang National University) ;
  • Jung, Ha-Na (Division of Environmental Forest Science, Gyeongsang National University) ;
  • Kim, Kyu-Sick (Department of Plant Conservation, Korea National Arboretum) ;
  • Choi, Myung-Suk (Division of Environmental Forest Science, Gyeongsang National University)
  • Published : 2007.10.31
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Abstract

An effective rapid propagation method was established through in vitro cultures of the medicinal plant, Cudrania tricuspidata. In vitro plantlets were obtained from in vitro germinated seeds. The various levels of cytokinins (BAP, Kinetin and TDZ) were tested on multiple shoot formation from plantlets. BAP (1.0 mg/l) treatment induced highest number of multiple shoots. Single shoot cultures gave higher initial shoot numbers than 5 shoots per culture. Among the various culture media, the shoot elongation was optimal on 2 MS basal medium without growth regulators. The IAA (2.0 mg/l) treatment induced highest number of roots. IBA (2.0 mg/l) treatment more promoted in vitro root growth than other concentrations. Rooted shoots were transferred directly to small pots with an artificial soil and successfully acclimatized.

Keywords

References

  1. Choi MS, Kwak SS, Liu JR, Lee IK, Yoo ID (2001) Production of novel flavonohds in cell cultures of Cudrania tricuspidata. Korean J. Plant Tissue Cult.28(3):159-164
  2. Fujimoto T, Hano Y, Nomura T (1984) Components of Root Bark of Cudrania tricuspidata 1.1,2 Structures of Four New Isoprenylated Xanthones, Cudraxanthones A, B, C and D. Planta Med 50:218-221 https://doi.org/10.1055/s-2007-969682
  3. Gamborg OL, Miller RA, Ojimaki K (1968) Nutrient require¬ments of suspension cultures of soybean root cells. Exp. Cell Res. 50:51-158
  4. Gomez MP, Segura J (1995) Axillary shoot proliferation in cultures of explants from mature Juniperus oxycedrus trees. Tree Physiol. 15:625-628 https://doi.org/10.1093/treephys/15.9.625
  5. Gurel S, Gulsen Y (1998) The effects of different sucrose, agar and pH levels on in vitro shoot production of almond (Amygdalus communis L.). Tr. J. Botany 22:363-373
  6. He SS, Liu CZ, Saxena PK (2007) Plant regeneration of an endangered medicinal plant Hydrastis canadensis L. Scientia Horticulturae 113(5):82-86 https://doi.org/10.1016/j.scienta.2007.01.014
  7. Kang DG, Hur TY, Lee GM, Oh H, Kwon TO, Sohn EJ, Lee HS. (2002) Effects of Cudrania tricuspidata water extract on blood pressure and renal functions in NO-dependent hypertension. Life Sciences, 70(22):2599-2609 https://doi.org/10.1016/S0024-3205(02)01547-3
  8. Lee IK, Kim CJ, Song KS, Kim HM, Koshino H, Uramoto M, Yoo ID (1996) Cytotoxic benzyl dihydroflavonols from Cudrania tricuspidata. Phytochem 41:213-216 https://doi.org/10.1016/0031-9422(95)00609-5
  9. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant 18:100-127 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x
  10. Lloyed GB, McCown BH (1981) Commercially feasible micropropagation of mountain laurel (Kalmia laticolia) by used of shoot tip culture. Proc. Int. Plant Propagators Soc. 30:421-437
  11. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  12. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85-87 https://doi.org/10.1126/science.163.3862.85
  13. Ostrolucka MG, Libiakova G, Ondruskova E, Gajdosova A (2004) In vitro propagation of Vaccinium species. Acta Universitatis Latviensis, Biology 676:207-212
  14. Quoirin M, Lepoivre P (1977) Improved media for in vitro culture of Prunus sp. Acta Hort. 78:437-442
  15. Rao MS, Purohit SD (2006) In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd. Biol. Plantarum 50:501-506 https://doi.org/10.1007/s10535-006-0079-0
  16. Schenk RU, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyle-donous plant cell cultures. Can. J. Bot. 50:199-204 https://doi.org/10.1139/b72-026
  17. Skirvin RM, Chu MC, Young HJ (1990) Rose. In: Ammirato, P.Y., Evans, D.R., Sharp, W.R. and Bajaj, Y.P.S., Editors, Handbook of Plant Cell Cultures, Vol. 5, MacMillan, New York p. 716-43
  18. Vuylsteke D (1989) Shoot-tip culture for the propagation, conservation and exchange of Musa germplasm. IBPGR, Rome
  19. White PR (1963) The cultivation of animal and plant cells. Ronald Press, New York. p. 199-203