DOI QR코드

DOI QR Code

Changes in Physiochemical Properties during the Fermentation of Doenjang Prepared with Black Soybeans

  • Park, Sung-Sun (Department of Food and Nutrition, Sungshin Women's University) ;
  • Oh, Sung-Hoon (Department of Food Science and Biotechnology, Ansan College of Technology) ;
  • Choi, Won-Dai (Agricultural Cooperative College) ;
  • Ra, Kyung-Soo (Department of Food and Nutrition, Daegu Technical College) ;
  • Suh, Hyung-Joo (Department of Food and Nutrition, Korea University)
  • Published : 2007.12.31

Abstract

A physicochemical assessment of Doenjang (traditional fermented soyfood) prepared with Korean black soybeans (Glycine max) was carried out. The T-N rate increased slowly during storage up to 120 days and the A-N rate increased up to 80 days of ripening and then decreased slightly. The caseinolytic activity increased slowly during storage up to 80 days and then decreased after 80 days. In addition, the fibrinolytic and ${\beta}-glucosidase$ activities increased up to 80 and 30 days and then decreased. Genistin and daidzin concentrations gradually decreased with increased fermentation time. However, genistein and daidzein slowly increased with fermentation time. Genistein and daidzein reached maximum concentrations (316.8 and $305.2{\mu}g/g$, respectively) and plateaued thereafter. The anthocyanins increased greatly during fermentation up to 50 days and then remained constant between 50 and 90 days. Polyphenol contents showed a slight increase up to 80 days and then slowly decreased. The DPPH and ABTS radical scavenging activities increased linearly during storage up to 50 days, reached about 28.9% and 2.17 mg/g, respectively, and then slowly decreased. At 20 days of fermentation, macrophage-stimulating activity of the extract showed a maximum activity.

Keywords

References

  1. Kwon TW, Song YS, Kim JS, Moon GS, Kim JI, Hong JH. 1998. Current research on the bioactive function of soyfoods in Korea. Korea Soybean Digest 15: 1-12
  2. Choung MG, Baek IY, Kang ST, Han WY, Shin DC, Moon HP, Kang KH. 2001. Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.). J Agric Food Chem 49: 5848-5851 https://doi.org/10.1021/jf010550w
  3. Rao AV, Sung MK. 1995. Saponins as anticarcinogens. J Nutr 125: s717-s724
  4. Miyazawa M, Sakano K, Nakamura S, Kosaka H. 1999. Antimutagenic activity of isoflavones from soybean seeds (Glycine max Merrill). J Agric Food Chem 47: 1346-1349 https://doi.org/10.1021/jf9803583
  5. Cardador-Martinez A, Castano-Tostado E, Loarca-Pina G. 2002. Antimutagenic activity of natural phenolic compounds present in the common bean (Phaseolus vulgaris) against aflatoxin B1. Food Addit Contam 19: 62-69 https://doi.org/10.1080/02652030110062110
  6. Aparicio-Fernandez X, Manzo-Bonilla L, Loarca-Pina G. 2005. Comparison of antimutagenic activity of phenolic compounds in newly harvested and stored common beans Phaseolus vulgaris against aflatoxin B1. J Food Sci 70: S73-S78 https://doi.org/10.1111/j.1365-2621.2005.tb09068.x
  7. Su YC. 1980. Traditional fermented food in Taiwan. In Proceedings of the Oriental Fermented Foods. Food industry research and development institute, Taipei, Taiwan. p 15
  8. Li ST. 1990. Ben-Tsao Gong-Mu (Chinese Botanical Encyclopedia). Great Taipei publishing Co., Taipei, Taiwan
  9. Harerkate F, Traas DW. 1974. Dose response curves in the fibrin plate assay to determined the fibrinolytic activity of proteases. Thromb Haemost 32: 357-365
  10. Murata Y, Satake M, Suzuki T. 1963. Studies on snake venom: XII. Distribution of protease activities among Japanese and Formosan snake venoms. J Biochem 53: 431-437 https://doi.org/10.1093/oxfordjournals.jbchem.a127719
  11. Peralta RM, Kadowaki MK, Terenzi HF, Jorge JA. 1997. A highly thermostable $\beta$-glucosidase activity from the thermophilic fugus humicola-grisea var. thermopedia: purification and biochemical characterization. FEMS Microbiol Letter 146: 291-295 https://doi.org/10.1016/S0378-1097(96)00490-9
  12. Adler-Nissen J. 1979. Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzenosulfonic acid. J Agric Food Chem 27: 1256-1262 https://doi.org/10.1021/jf60226a042
  13. AOAC. 1975. Official Methods of Analysis. Association of Official Analytical Chemists, Washington, DC
  14. Coward L, Barnes NC, Setchell KDR, Barnes S. 1993. Genistein, daidzein, and their β-glucoside conjugates: antitumor isoflavones in soybean foods from American and Asian diets. J Agric Food Chem 41: 1961-1967 https://doi.org/10.1021/jf00035a027
  15. Waterman PG, Mole S. 1994. Analysis of phenolic plant metabolites. Blackwell Scientific Publication, Oxford. p 83-91
  16. Somers TC, Evans ME. 1977. Spectral evaluation of young red wines: Anthocyanin equilibria, total phenolics, free and molecular SO2 chemical age. J Sci Food Agric 28: 279-287 https://doi.org/10.1002/jsfa.2740280311
  17. Zoecklein BW, Fugelsang KC, Gump BH, Nury FS. 1990. Production wine analysis. Van Nostrand Reinhold Publication, New York. p 129-168
  18. Conrad RE. 1981. Induction and collection of peritoneal exudates macrophages. In Manual of Macrophage Methodology. Herscowitz BH, Holden HT, Bellanti JA, Ghaffar A, eds. Marcel Dekker, New York, NY. p 5-11
  19. Suzuki I, Tanaka H, Kinoshita A, Oikawa S, Osawa M, Yadomae T. 1990. Effect of orally administered $\beta$-glucan on macrophage function in mice. Int J Immunopharmacol 12: 675-684 https://doi.org/10.1016/0192-0561(90)90105-V
  20. Rhee CH, Kim WC, Rhee IK, Lee OS, Park HD. 2006. Changes in the physicochemical property, angiotensin converting enzyme inhibitory effect and antimutagenicity during the fermentation of Korean traditional soy paste (Doenjang). Korean J Food Preserv 13: 603-610
  21. Neurath H. 1989. Proteolytic enzymes. In Proteolytic Enzymes - A Practical Approach. Beynon RJ, Bond JS, eds. IRL Press, Oxford. p 1-13
  22. Bode C, Runge M, Samlling RW. 1996. The future of thrombolysis in the treatment of acute myocardial infarction. Eur Heart J 17: 55-60 https://doi.org/10.1093/eurheartj/17.suppl_E.55
  23. Lee IH, Chou CC. 2006. Distribution profiles of isoflavone isomers in black bean kojis prepared with various filamentous fungi. J Agric Food Chem 54: 1309-1314 https://doi.org/10.1021/jf058139m
  24. McCue P, Shetty K. 2003. Role of carbohydrate-cleaving enzymes in phenolic antioxidants mobilization from whole soybean fermented with Rhizopus oligosporus. Food Biotech 17: 27-37 https://doi.org/10.1081/FBT-120019982
  25. Hung YH. 2006. Antimutagenic activity and mechanism of black soybean koji. MS Thesis. National Taiwan University, Taipei, Taiwan
  26. Tikkanen MJ, Wahala K, Ojala S, Vihma V, Adlercreutz H. 1998. Effect of soybean phytoestrogen intake on low density lipoprotein oxidation resistance. P Natl Acad Sci USA 95: 3106-3110 https://doi.org/10.1073/pnas.95.6.3106
  27. Tikkanen MJ, Adlecreutz H. 2000. Dietary soy-derived isoflavone phytoestrogens. Could they have a role in coronary heart disease prevention? Biochem Pharmacol 60: 1-5 https://doi.org/10.1016/S0006-2952(99)00409-8
  28. Barnes S, Coward L, Kirk M, Sfakianos J. 1998. HPLC–mass spectrometry analysis of isoflavones. Proc Soc Exp Biol Med 217: 254-262
  29. Allred CD, Ju YH, Allred KF, Cang J, Helferich WG. 2001. Dietary genistin stimulates growth of estrogen-dependent breast cancer tumors similar to that observed with genistein. Carcinogenesis 22: 1667-1673 https://doi.org/10.1093/carcin/22.10.1667
  30. Piskula MK, Yamakoshi J, Iwai Y. 1999. Daidzein and genistein but not their glucosides are absorbed from the rat stomach. FEBS Lett 47: 287-291 https://doi.org/10.1016/S0014-5793(99)00307-5
  31. Setchell DRK, Brown NM, Zimmer-Nechemias L, Brashear WT, Wolfe BE, Kirschner AS, Heubi JE. 2002. Evidence for lack of absorption of soy isoflavone glycosides in humans, supporting the crucial role of intestinal metabolism for bioavailability. Am J Clin Nutr 76: 447-453 https://doi.org/10.1093/ajcn/76.2.447
  32. Markham KR, Ternai B, Stanley R, Geiger H, Mabry TJ. 1978. Carbon-13 NMR studies of flavonoids-III naturally occurring flavonoid glycosides and their acylated derivatives. Tetrahedron 34: 1389-1397 https://doi.org/10.1016/0040-4020(78)88336-7
  33. McCue P, Shetty K. 2003. Role of carbohydrate-cleaving enzymes in phenolic antioxidants mobilization from whole soybean fermented with Rhizopus oligosporus. Food Biotech 17: 27-37 https://doi.org/10.1081/FBT-120019982
  34. Randhir R, Vattem D, Shetty K. 2004. Solid-state bioconversion of fava bean by Rhizopus oligosorus for enrichment of phenolic antioxidants and L-DOPA. Innovat Food Sci Emerg Tech 5: 235-244 https://doi.org/10.1016/j.ifset.2004.01.003
  35. Ruiz-Larrea MB, Mohan AR, Paganga G, Miller NJ, Bolwell GP, Rice-Evans CA. 1997. Antioxidant activity of phytoestrogenic isoflavones. Free Radical Res 26: 63-70 https://doi.org/10.3109/10715769709097785
  36. Stoll BA. 1997. Eating to beat breast cancer: potential role for soy supplements. Annals Oncology 8: 223-225 https://doi.org/10.1023/A:1008237505645
  37. Arora A, Nair MG, Strasburg GM. 1998. Antioxidant activities of isoflavones and their biological metabolites in a liposomal system. Arch Biochem Biophys 356: 133-141 https://doi.org/10.1006/abbi.1998.0783
  38. Lee CH, Yang L, Xu JZ, Yeung SYV, Huang Y, Chen ZY. 2005. Relative antioxidant activity of soybean isoflavones and their glycosides. Food Chem 90: 735-741 https://doi.org/10.1016/j.foodchem.2004.04.034
  39. Mitchell JH, Gardner PT, McPhail DB, Morrice PC, Collins AR, Duthie GG. 1998. Antioxidant efficacy of phytoestrogens in chemical and biological model systems. Arch Biochem Biophys 360: 142-148 https://doi.org/10.1006/abbi.1998.0951
  40. Wang H, Nair MG, Strasburg GM, Chang YC, Booren AM, Gray JI, Dewitt DL. 1999. Antioxidant and anti-inflammatory activities of anthocyanins and their aglycon, cyaniding, from tart cherries. J Nat Prod 62: 294-296 https://doi.org/10.1021/np980501m
  41. Cignarella A, Nastasi M, Cavalli E, Puglisi L. 1996. Novel lipidlowering properties of Vaccinium myrtillus L. leave, a traditional antidiabetic treatment, in several models of rat dyslipidaemia: a comparison with ciprofibrate. Thromb Res 84: 311-322 https://doi.org/10.1016/S0049-3848(96)00195-8
  42. Brouillard R. 1988. Flavonoids and flower color. In The Flavonoids. Harborne JB, ed. Chapman & Hall, London. p 525-538
  43. Sunderkotter C, Steinbrink K, Goebeler M, Bhardwaj R, Sorg C. 1994. Macrophages and angiogenesis. J Leukocyte Biol 55: 410-422 https://doi.org/10.1002/jlb.55.3.410
  44. Morel F, Doussiere J, Vignais PV. 1991. The superoxide generating oxidase of phagocytic cells. Physiological, molecular and pathological aspects. Eur J Biochem 201: 523-546 https://doi.org/10.1111/j.1432-1033.1991.tb16312.x
  45. Ramesh HP, Yamaki K, Tsushida T. 2002. Effect of fenugreek (Trigonella foenum-graecum L.) galactomannan fractions on phagocytosis in rat macrophages and on pro liferation and IgM secretion in HB4C5 cells. Carbohydr Polym 50: 79-83 https://doi.org/10.1016/S0144-8617(01)00377-0
  46. Ferencik M, Stvrtinova V. 1995. Endogenous control and modulation of inflammation. Folia Biol (Praha) 42: 47- 55

Cited by

  1. Quality Characteristics of Black Soybean Paste (Daemacjang) with Mixture Ratio of Black Soybean, Barley and Salt Concentration vol.26, pp.2, 2013, https://doi.org/10.9799/ksfan.2013.26.2.266
  2. Evaluation of Quality Characteristics and Antioxidant Activities from Doenjang Ripened for 30 Years vol.48, pp.4, 2014, https://doi.org/10.14397/jals.2014.48.4.253
  3. Optimization of Processing Condition for Antioxidant Activities from Aged Black Ginger Using Response Surface Methodology vol.54, pp.6, 2020, https://doi.org/10.14397/jals.2020.54.6.99