Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Antinociceptive profile of the ethanolic extract of andrographis paniculata in mice

  • Sulaiman, MR (Laboratory of Natural Products, Institute of Biosciences, Universiti Putra Malaysia) ;
  • Sainan, S (Laboratory of Natural Products, Institute of Biosciences, Universiti Putra Malaysia) ;
  • Zakaria, ZA (Department of Biomedical Sciences, Faculty of Medicine Health Sciences, Universiti Putra Malaysia) ;
  • Somchit, MN (Laboratory of Natural Products, Institute of Biosciences, Universiti Putra Malaysia) ;
  • Israf, DA (Laboratory of Natural Products, Institute of Biosciences, Universiti Putra Malaysia) ;
  • Moin, S (Laboratory of Natural Products, Institute of Biosciences, Universiti Putra Malaysia) ;
  • Mohamad, TA Tengky (Department of Biomedical Sciences, Faculty of Medicine Health Sciences, Universiti Putra Malaysia)
  • Published : 2007.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was conducted to evaluate the analgesic activity of ethanolic extract of Andrographis paniculata (AP) in mice. The analgesic investigations were carried out using the acetic acid-induced abdominal writhing and the hot-plate tests. It was demonstrated that intraperitoneal (i.p.) administration of the extract at a dose of 30, 100, 300, 500 mg/kg, produced significant inhibition of abdominal constriction induced with 0.6% (v/v) acetic acid in dosedependent manner. It also demonstrated that the extract produced significant dose-dependent increase in the time of latency to a discomfort reaction in the hot-plate model. In addition, the analgesic effect of the ethanolic extract of AP was significantly reversed by a non-specific opioid receptor antagonist, naloxone. These results indicate that AP has an analgesic effect that was mediated through opioid receptors.

Keywords

References

  1. Collier HOJ, Dinneen LC, Johnson CA, Schneider C. (1968) The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br. J. Pharmacol. 32, 295-310
  2. Gabrielian ES, Shukarian AK, Goukasova GI, Chandanian GL, Panossian AG, Wikman G, Wagner H. (2002) A double blind, placebo-controlled study of Andrographis paniculata fixed combination Kan Jang in the treatment of acute upper respiratory tract Infections including sinusitis. Phytomedicine 9, 589-597 https://doi.org/10.1078/094471102321616391
  3. Gene RM, Segura L, Adzet T, Marin E, Iglesias J. (1998) Heterotheca inoloides: anti-inflammatory and analgesic effect. J. Ethnopharmacol. 60, 157-160 https://doi.org/10.1016/S0378-8741(97)00155-4
  4. Goh SH, Liew SL, Soepadmo E. (1988) Malaysian Medicinal Plants Used For Treatment of Hypertension. Proceedings: Malaysian Traditional Medicine, Kuala Lumpur. pp. 104-110
  5. Handa SS, Sharma A. (1990) Hepatoprotective activity of andrographolide against galactosamine & paracetamol intoxication in rats. Indian J. Med. Res. 92, 284-292
  6. Hosseinzabeh H, Ramezani M, Salmani G. (2000) Antinociceptive, anti-inflammatory and acute toxicity effects of Zataria multiflora Boiss extracts in mice and rats. J. Ethanopharmacol. 73, 379-385 https://doi.org/10.1016/S0378-8741(00)00238-5
  7. Jain SR, Sharma SN. (1967) Hypoglycaemia of India Indigenous Origin. Planta Med. 15, 439-442
  8. Koster R, Anderson M, De Beer EJ. (1959) Acetic acid for analgesic screening. Fed. Proc. 18, 418-420
  9. Leelarasamee A, Trakulsomboon S, Sittisomwong N. (1990) Undetectable anti-bacterial activity of Andrographis paniculata (Burma) wall. ex ness. J. Med. Assoc. Thai. 73, 299-304
  10. Shen YC, Chen CF, Chiou WF. (2002) Andrographolide prevents oxygen radical production by human neutrophils: possible mechanism(s) involved in its anti-inflammatory effect. Br. J. Pharmacol. 135, 399-406 https://doi.org/10.1038/sj.bjp.0704493
  11. Somchit MN, Reezal I, Nur IE, Mutalib AR. (2003) In vitro antimicrobial activity of ethanol and water extracts of Cassia alata. J. Ethnopharmacol. 84, 1-4 https://doi.org/10.1016/S0378-8741(02)00146-0
  12. Thamlikitkul V, Dechatiwongse T, Theerapong S, Chantrakul C, Boonroj P, Punkrut W, Ekpalakorn W, Boontaeng N, Taechaiya S, Petcharoen S. (1991) Efficacy of Andrographis paniculata, Nees for pharyngotonsillitis in adults. J. Med. Assoc. Thai. 74, 437-442
  13. Vogel HG, Vogel WH. (1997) Drug Discovery and Evaluation: Pharmacological Assays. Springer- Verlaag, Berlin, Heidelberg; pp. 360-418
  14. Visen PK, Shukla B, Patnaik GK, Dhawan BN. (1993) Andrographolide protects rat hepatocytes against paracetamol-induced damage. J. Ethnopharmacol. 40, 131-136 https://doi.org/10.1016/0378-8741(93)90058-D
  15. Zhang CY, Tan BK. (1996). Hypotensive activity of aqueous extract of Andrographis paniculata in rats. Clin. Exp. Pharmacol. Physiol. 23, 675-678 https://doi.org/10.1111/j.1440-1681.1996.tb01756.x
  16. Zhang XF, Tan BK. (2000) Anti-diabetic property of ethanolic extract of Andrographis paniculata in streptozotocin-diabetic rats. Acta Pharmacol. Sin. 21, 1157-1164
  17. Zimmermann M. (1983) Ethical guidelines for investigation of experimental pain in conscious animals. Pain 16, 109-110 https://doi.org/10.1016/0304-3959(83)90201-4