이묘산(二妙散)에 의한 대장암 세포주 HCT116의 Caspases 활성화를 매개로 한 세포사멸

Imyosan induces caspases-mediated apoptosis in human colorectal cancer HCT116 cells

  • 김선모 (동국대학교 한의과대학 방제학교실) ;
  • 윤현정 (동국대학교 한의과대학 방제학교실) ;
  • 이현우 (동국대학교 한의과대학 방제학교실) ;
  • 김판준 (동국대학교 한의과대학 방제학교실) ;
  • 이창현 (동국대학교 한의과대학 방제학교실) ;
  • 박원환 (동국대학교 한의과대학 방제학교실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실)
  • Kim, Sun-Mo (Department of Prescriptionology, College of Oriental Medicine, Dongguk University) ;
  • Yun, Hyun-Jeung (Department of Prescriptionology, College of Oriental Medicine, Dongguk University) ;
  • Lee, Hyun-Woo (Department of Prescriptionology, College of Oriental Medicine, Dongguk University) ;
  • Kim, Pan-Jun (Department of Prescriptionology, College of Oriental Medicine, Dongguk University) ;
  • Lee, Chang-Hyun (Department of Prescriptionology, College of Oriental Medicine, Dongguk University) ;
  • Park, Won-Hwan (Department of Prescriptionology, College of Oriental Medicine, Dongguk University) ;
  • Park, Sun-Dong (Department of Prescriptionology, College of Oriental Medicine, Dongguk University)
  • 발행 : 2006.12.30

초록

The purpose of this study was to investigate the effect of Imyosan on apoptosis in human colorectal cancer HCT116 cells. Phellodendron amurense Rupr. and Atratylodes lancea D.C. compose Imyosan. First of all, to study the cytotoxic effect of methanol extract of Imyosan (IMS-MeOH) on HCT116 cells, the cells were treated with various concentrations of IMS-MeOH and then cell viability was determined by XTT reduction method. IMS-MeOH reduced viability of HCT116 cells in a dose and time-dependent manner. To confirm the induction of apoptosis, the c1eavage of poly ADP-ribose polymerase (PARP), a substrate for caspase-3 and a typical sign of apoptosis, and the activation of caspase-3, procaspase-8 and procaspase-9 were examined by western blot analysis. IMS-MeOH decreased procaspase-3, procaspase-8 and procaspase-9 levels in a dose-dependent manner and induced the clevage of PARP. IMS-MeOH triggered the mitochondrial apoptotic signaling by increasing the release of cytochrome c from mitochondria to cytosol. Furthermore, IMS-MeOH also downregulated the anti-apoptotic Bcl-2 and upregulated the pro-apoptotic-Bax. Therefore, these results suggest that IMS-MeOH induced HCT1l6 cell death through the mitochondrial pathway. To explore whether the activities of caspases was required for induction of apoptosis by IMS-MeOH, caspase-3, -8, -9 activity measured by using substrates, respectively. IMS-MeOH increased caspase-3, -8, -9 activity. Co-treatment with inhibitors of caspase-3, -8, -9 and IMS-MeOH significantly blocked IMS-MeOH-triggered apoptosis in HCT1l6 cells. These results suggest that IMS-MeOH induces caspases-mediated apoptosis.

키워드