한국한의학연구원논문집 (Korean Journal of Oriental Medicine)
- 제12권3호통권18호
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- Pages.131-141
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- 2006
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- 1229-3377(pISSN)
자하거(紫河車) 약침액(藥鍼液)이 Lipopolysaccharide로 처리된 RAW 264.7 대식세포주(大食細胞柱)의 유전자(遺傳子) 발현(發顯)에 미치는 영향(影響)
Microarray Analysis of Gene Expression in Raw 264.7 Cells Treated with Hominis Placenta Herbal-Acupuncture Solution
- 장현석 (대구한의대학교 한의과대학) ;
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이경민
(대구한의대학교 한의과대학) ;
- 임성철 (대구한의대학교 한의과대학) ;
- 엄동명 (한국한의학연구원) ;
- 서정철 (대구한의대학교 한의과대학)
- Jang, Hyun-Seok (College of Oriental Medicine, Daegu Haany University) ;
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Lee, Kyung-Min
(College of Oriental Medicine, Daegu Haany University) ;
- Lim, Sung-Chul (College of Oriental Medicine, Daegu Haany University) ;
- Eom, Dong-Myung (Korea Institute of Oriental Medicine) ;
- Seo, Jung-Chul (College of Oriental Medicine, Daegu Haany University)
- 발행 : 2006.12.30
초록
Hominis Placenta has a broad array of clinical applications in Korean medicine, including treatment of inflammatory conditions such as rheumatoid arthritis. The purpose of this study is to explore the global gene expression profiles in human RAW 264.7 cell lines treated with Hominis Placenta herbal-acupuncture solution (HPHAS) using microarray analysis. The RAW 264.7 cells were treated with lipopolysaccharide (LPS), HPHAS, or both. Of the 8,170 genes profiled in this study, with a cut-off level of two-fold change in the expression, 72 genes (CTD1, regulating synaptic membrane exocytosis 2, etc.) were upregulated and 135 genes(splicing factor, arginine/serine-rich 1, actinin, alpha 1, etc.) downregulated following LPS treatment. One gene (acrosin) was upregulated and 12 genes (phospholipase A2, group IB, neurofilament, heavy polypeptide 200kDa, etc.) were downregulated following HPHAS treatment. Eleven genes (RAB27A, member RAS oncogene family, eosinophil peroxidase, etc.) were upregulated and 16 genes (V-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian), RW1 protein, etc.) were downregulated following co-stimulation of HPHAS and LPS. It is thought that microarrays will play an ever-growing role in the advance of our understanding of the pharmacological actions of HPHAS in the treatment of arthritis. Further studies, however, are required to concretely prove the effectiveness of HPHAS.