Parasites, Hosts and Diseases

  • 제44권3호
  • /
  • Pages.233-237
  • /
  • 2006
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지

Cloning of the novel putative apoptosis-related gene of Spirometra erinacei (Order Pseudophyllidea)

  • Lee Soo-Ung (Department of Parasitology, College of Medicine, Hallym University) ;
  • Huh Sun (Department of Parasitology, College of Medicine, Hallym University)
  • 발행 : 2006.09.01
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초록

We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.

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참고문헌

  1. Ameisen JC, Idziorek T, Billaut-Mulot O, Loyens M, Tissier JP, Potentier A, Ouaissi A (1995) Apoptosis in a unicellular eukaryote (Trypanosoma cruzi): implication for the evolutionary origin and role of programmed cell death in the control of cell proliferation, differentiation and survival. Death and Differentiation 2: 285-300
  2. Bendtsen JD, Nielsen H, von Heijne G, Brunak S (2004) Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340: 783-795 https://doi.org/10.1016/j.jmb.2004.05.028
  3. Van de Craen M, Vandenabeele P, Declercq W, Van den Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W, Beyaert R, Fiers W (1997) Characterization of seven murine caspase family members. FEBS Letter 403: 61-69 https://doi.org/10.1016/S0014-5793(97)00026-4
  4. Grand RJ (1989) Acylation of viral and eukaryotic proteins. Biochem J 258: 625-638 https://doi.org/10.1042/bj2580625
  5. Hengartner MO, Horvitz HR (1994) C. elegans cell survival gene ced-9 encodes a functional homolog of the mammalian proto-oncogene bcl-2. Cell 76: 665-676 https://doi.org/10.1016/0092-8674(94)90506-1
  6. Hulo N, Sigrist CJ, Le Saux V, Langendijk-Genevaux PS, Bordoli L, Gattiker A, De Castro E, Bucher P, Bairoch A (2004) Recent improvements to the PROSITE database. Nucleic Acids Res 32: D134-137
  7. Kerr JF, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26: 239-257 https://doi.org/10.1038/bjc.1972.33
  8. Nielsen H, Engelbrecht J, Brunak S, von Heijne G (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 10: 1-6 https://doi.org/10.1093/protein/10.1.1
  9. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, New York, USA
  10. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467
  11. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680 https://doi.org/10.1093/nar/22.22.4673
  12. Thornberry NA, Bull HG, Calaycay JR, Chapman KT, Howard AD, Kostura MJ, Miller DK, Molineaux SM, Weidner JR, Aunins J (1992) A novel heterodimeric cysteine protease is required for interleukin-1${\beta}$, processing in monocytes. Nature 356: 768-774 https://doi.org/10.1038/356768a0
  13. Yuan JY, Horvitz HR (1990) The Caenorhabditis elegans genes ced-3 and ced-4 act cell autonomously to cause programmed cell death. Dev Biol 138: 33-41 https://doi.org/10.1016/0012-1606(90)90174-H
  14. Yuan JY, Shaham S, Ledoux S, Ellis HM, Horvitz HR (1993) The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1${\beta}$,-converting enzyme. Cell 75: 641-652 https://doi.org/10.1016/0092-8674(93)90485-9