The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Identification and Sequence Analysis of RNA3 of a Resistance-Breaking Cucumber mosaic virus Isolate on Capsicum annuum

  • Lee Mi-Yeon (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University) ;
  • Lee Jang-Ha (Biotechnology Institute, Nong Woo Bio Co. Ltd.) ;
  • Ahn Hong-Il (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University) ;
  • Yoon Ju-Yeon (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University) ;
  • Her Nam-Han (Biotechnology Institute, Nong Woo Bio Co. Ltd.) ;
  • Choi Jang-Kyung (Division of Biological Environment, Kangwon National University) ;
  • Choi Gug-Seon (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim Do-Sun (National Horticultural Research Institute, Rural Development Administration) ;
  • Harn Chee-Hark (Biotechnology Institute, Nong Woo Bio Co. Ltd.) ;
  • Ryu Ki-Hyun (Plant Virus GenBank, Division of Environmental & Life Sciences, Seoul Women's University)
  • Published : 2006.09.01
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Abstract

Cultivated hot pepper crops showing severe mosaic symptom were found in Korea in 2004 and their causal agent was identified as Cucumber mosaic virus (CMV). These pepper crops was resistant to the virus in the filled, and they belonged to pathotype 0 (P0) resistant pepper. Resistance screening of selected pepper plants showed that a pepper isolate of CMV was the P0 resistance-breaking virus. This P0 resistance-breaking isolate of CMV, named as Ca-P1, was isolated from leaves of the virus-infected Capsicum annuum cv. Manidda that showed systemic severe mosaic symptom. Ca-P1-CMV could induce systemic mosaic symptoms on P0-susceptible (P0-S) and P0-resistant (P0-R) cultivars whereas an ordinary strain (Fny-CMV) could not infect P0-R. This result suggests that Ca-P1-CMV can overcome P0 resistant pepper cultivars. To analyze its genome sequence, the complete nucleotide sequence of RNA3 of Ca-P1-CMV was determined from the infectious full-length cDNA clone of the virus. RNA3 of Ca-P1-CMV consisted of 2,219 nucleotides. Overall sequence homology of RNA3-encoded two viral proteins (movement protein and coat protein) revealed high similarity (75.2-97.2%) with the known CMV strains. By sequence analysis with known representative strains of CMV, Ca-P1-CMV belongs to a typical member of CMV subgroup IB. The resistance and resistance-breaking mechanisms of pepper and counterpart CMV, respectively, remain to be investigated, which will enrich the genetic resources and accelerate CMV-resistant pepper breeding programs.

Keywords

References

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