Nutritional Sciences

The Korean Nutrition Society (한국영양학회)
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Inhibitory Effects of (-)Epigallocatechin Gallate and Quercetin on Phorbol 12-Myristate 13-Acetate-Induced Secretion of Metalloproteinase-2 and Metalloproteinase-9

  • Kang Sang-Wook (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University) ;
  • Choi Yean-Jung (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University) ;
  • Choi Jung-Suk (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University) ;
  • Kwon Hyang-Mi (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University) ;
  • Bae Ji-Young (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University) ;
  • Park Eun-Hee (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University) ;
  • Ji Geun-Eog (Department of Food and Nutrition, Seoul National University) ;
  • Kang Il-Jun (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University) ;
  • Kang Young-Hee (Department of Food and Nutrition and Institute of Nutrition Research, Hallym University)
  • Published : 2006.08.01
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Abstract

Matrix metalloproteinases (MMP) play an important role in the extracellular matrix (ECM) degradation undetphysiological and pathological conditions. The present study examined the influence of (-)epigallocatechin gallate and quercetin on phorbol-12-myristate 13-acetate (PMA)-induced secretion of MMP-2 and MMP-9, when human umbilical vein endothelial cells (HUVEC) were treated with (-)epigallocatechin gallate and quercetin at supraphysiological concentrations of $25{\mu}mol/L$. No cytotoxicity was observed by MIT assay in response to a treatment with PMA in the presence of (-)epigallocatechin gallate and quercetin. Western blot analysis and gelatin zymography revealed that exposure of HUVEC to PMA enhanced the levels and gelatinolytic activities of pro and active forms of MMP-2 and active form of MMP-9. (-)Epigallocatechin gallate attenuated PMA-stimulated secretion of active forms of MMP-2 and MMP-9 concomitantly with a loss of activities of these enzymes, which was related to the decreased mRNA levels of MMP. Quercetin was more potent than (-)epigallocatechin gallate in alleviating MMP-9 protein secretion and activity with a decrease in MMP-9 mRNA accumulation. Taken together, the results indicated that (-)epigallocatechin gallte and quercetin exhibited inhibitory effects on MMP activity and may qualify as chemopreventive and cardiovascular protective agents.

Keywords

References

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