Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Stimulation of Platelet-Activating Factor (PAF) Synthesis in Human Intestinal Epithelial Cell Line by Aerolysin from Aeromonas encheleia

  • Nam In-Young (Departments of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Cho Jae-Chang (Departments of Environmental Science, Hankuk University of Foreign Studies) ;
  • Myung Hee-Joon (Departments of Bioscience and Biotechnology, Hankuk University of Foreign Studies) ;
  • Joh Ki-Seong (Departments of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
  • Published : 2006.08.01
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Abstract

Aeromonas encheleia, a potential human intestinal pathogen, was shown to infect a human intestinal epithelial cell line (Caco-2) in a noninvasive manner. The transcriptional profile of the Caco-2 cells after infection with the bacteria revealed an upregulated expression of genes involved in chloride secretion, including that of phospholipase A2 (PLA2) and platelet-activating factor (PAF) acetylhydrolase (PAFAH2). This was also confirmed by a real-time RT-PCR analysis. As expected from PLA2 induction, PAF was produced when the Caco-2 cells were infected with the bacteria, and PAF was also produced when the cells were treated with a bacterial culture supernatant including bacterial extracellular proteins, yet lacking lipopolysaccharides. Bacterial aerolysin was shown to induce the production of PAF.

Keywords

References

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