Journal of Microbiology and Biotechnology

  • 제16권7호
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  • Pages.1017-1025
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  • 2006
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Generation and Segregation of Hantaviral RNA Genomic Diploid; Implications of Reassortant Generation Mechanism

  • Park Sun-Whan (Department of Microbiology, College of Medicine,Korea University) ;
  • Chung Dong-Hoon (Southern Research Institute 2000) ;
  • Ahn Byung-Yoon (School of Life Sciences and Biotechnology, Korea University) ;
  • Lee Pyung-Woo (Department of Microbiology, College of Medicine, Korea University)
  • 발행 : 2006.07.01
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초록

Hantaviruses possess three RNA segments of negative sense. Co-infection of closely related hantaviruses may result in generation of a progeny virus with genomic polyploidy, containing a partial or complete set of genome originated from more than one parental virus. To characterize the formation of viral genomic polyploidy, cultured Vero-E6 cells were co-infected with two closely related hantaviruses, Hantaan and Maaji, and the progeny viruses examined. The genotype of plaque-purified viruses was analyzed by a virus-specific RT-PCR. Seventy percent (67/96) of the progeny virus was categorized as Hantaan and 3.3% (2/96) was classified as Maaji, whereas 20% (21/96) was considered polyploidy as they contained both types of the S RNA segment. Most of the polyploidy progeny viruses were unstable and gave rise to either one of the parental viruses or a reassortant after several rounds of plaque purification. No recombination between the heterologous pair of S RNA was observed for those polyploid viruses during three consecutive plaque-to-plaque passages. These data suggest that the viral polyploidy formation constitutes a primary mechanism underlying the generation of a newly emerged hantavirus.

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