Protective Effects of Hemerocallis Fulva Extracts on Amyloid $\beta$-Protein-Induced Death in Neuronal Cells

아밀로이드 베타 단백질에 의해 유도된 신경세포 독성에 대한 원추리의 억제 효과 탐색

  • Kim Eun-Sook (Department of Food Science & Technology, College of Engineering, Kyungsung University) ;
  • Choi Soo-Jin (Department of Food Science & Technology, College of Engineering, Kyungsung University) ;
  • Ryu Beung-Ho (Department of Food Science & Technology, College of Engineering, Kyungsung University) ;
  • Choi Jin-Ho (Division of Food Science and Biotechnology, College of Fisheries Sciences, Pukyong National University) ;
  • Oh Myung-Sok (Department of Life Science and National Research Laboratory of Proteolysis, Kwangju Institute of Science and Technology) ;
  • Park Woo-Jin (Department of Life Science and National Research Laboratory of Proteolysis, Kwangju Institute of Science and Technology) ;
  • Choi Young-Whan (Department of Horticultural Bioscience, College of Natural Resources and Life Science, Pusan National University) ;
  • Paik Do-Hyeon (Institute of NeoHerb Oriental Medicine Biotechnology) ;
  • Ha Kwon-Chul (Institute of NeoHerb Oriental Medicine Biotechnology) ;
  • Kang Dae-Ook (Institute of NeoHerb Oriental Medicine Biotechnology) ;
  • Cho Yong-Kweon (Institute of NeoHerb Oriental Medicine Biotechnology) ;
  • Park Ki-Tae (Institute of NeoHerb Oriental Medicine Biotechnology) ;
  • Moon Ja-Young (Department of Biochemistry and Health Sciences, Changwon National University)
  • 김은숙 (경성대학교 공과대학 식품공학과) ;
  • 최수진 (경성대학교 공과대학 식품공학과) ;
  • 류병호 (경성대학교 공과대학 식품공학과) ;
  • 최진호 (부경대학교 수산과학대학 식품생명공학부) ;
  • 오명석 (광주과학기술원 생명과학과) ;
  • 박우진 (광주과학기술원 생명과학과) ;
  • 최영환 (부산대학교 생명자원과학대학 생명자원과학부 원예학과) ;
  • 백도현 ((주)네오허브 한방생명공학연구소) ;
  • 하권철 ((주)네오허브 한방생명공학연구소) ;
  • 강대욱 ((주)네오허브 한방생명공학연구소) ;
  • 조용권 ((주)네오허브 한방생명공학연구소) ;
  • 박기태 ((주)네오허브 한방생명공학연구소) ;
  • 문자영 (창원대학교 자연과학대학 보건의과학과)
  • Published : 2006.06.30

Abstract

Objectives : The amyloid $\beta$-protein ($A\beta$) is the principal component of the senile plaques characteristic of Alzheimer's disease (AD) and elicits a toxic effect on neurons in vitro and in vivo. Many environmental factors including antioxidants and proteoglycans modify $A{\beta}toxicity$. In this study, we have investigated the protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on $A\beta$-induced oxidative cell death in cultured rat pheochromocytoma (PC12) cells. Methods : For this study, we used MTT reduction assay for detection of protective effects of water- and organic solvent-extracts of Hemerocallis fulva root fractions pre-extracted with methanol on $A{\beta}_{25-35}$-induced cytotoxicity to PC12 cells. We also used cell-based $\beta$-secretase assay system to investigate the inhibitory effect of water- and organic solvent-extracts of Hemerocallis fulva root on $\beta$-secretase activity. Results : We previously reported that methanol extracts of Hemerocallis fulva root strongly attenuated cytotoxicity induced by the three $A\beta$ fragments ($A{\beta}_{25-35},\;A{\beta}_{1-42}\;A{\beta}_{1-43}$) to both SK-N-MC and PC12 cells. In the present study, we found that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol had strong protective effects against $A{\beta}_{25-35}$-induced cytotoxicity to PC12 cells and inhibitory potency to $\beta$-secretase activity. Conclusion : These results suggest that butanol-, ethylacetate-, chloroform-, and water-extracts of Hemerocallis fulva root fractions pre-extracted with methanol may contain the protective component(s) against $A\beta$-induced cell death in PC12 cells as well as inhibitory component(s) to $\beta$-secretase activity.

Keywords