Development and Reproduction (한국발생생물학회지:발생과생식)
- Volume 10 Issue 2
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- Pages.115-125
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- 2006
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- 2465-9525(pISSN)
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- 2465-9541(eISSN)
Effect of Selenium on Oocyte Maturation and Viability in vitro in Mouse
체외 배양시 생쥐난자의 성숙과 생존에 미치는 Selenium의 영향
- Choi, Eun-Jin (Department of Life Science, Chungbuk National University) ;
- Hong, Soon-Gab (Department of Life Science, Chungbuk National University) ;
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Kim, Hae-Kwon
(Department of Biotechnology, Seoul Women's University) ;
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Yoon, Yong-Dal
(Department of Life Science, College of Natural Science, Hanyang University) ;
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Lee, Joon-Yeong
(Department of Life Science, Chungbuk National University)
- Published : 2006.10.01
Abstract
The present experiment was performed to confirm the effects of selenium on maturation and viability of mouse oocyte. Maturation of oocytes was observed by microscope, Germinal vesicle breakdown(GVBD) and polar body formation(PB) were confirmed at 2.5, 13 hours after in vitro culture. Viability of oocytes was observed by microscope. Normal and abnormal oocytes were distinguished by morphological change in vitro culture for 72 hours. Glutathione(GSH) content of collected oocytes from individual stage also was measured by glutathione assay using spectrophotometer. The results obtained were as follows; The low concentration of selenium(
본 실험은 생쥐 난자의 성숙과 생존에 미치는 selenium의 영향을 알아보고자 수행하였다. 난자의 성숙은 현미경을 통해 관찰하였으며, 핵막 붕괴(germinal vesicle breakdown, GVBD)와 극체 형성(polar body formation, PB)은 체외 배양 시작 후 각각 2.5, 13시간에 확인하였다. 난자의 생존은 72 시간동안 체외 배양하면서 형태학적 차이로 정상 난자와 비정상 난자를 판별하였다. 또한 각 단계별로 수집된 난자의 glutathione(GSH) 함량은 spectrophotometer를 사용하여 glutathione assay로 측정하였다. 결과는 다음과 같다; 저농도의 selenium(