Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Protective Effects of Activated Charcoal on the Mouse Hepatotoxicity Induced by Lead

납으로 유발된 생쥐 간독성에 대한 활성탄의 보호효과

  • Cheong, Min-Ju (Department of Biology, College of Natural Science, Chosun University) ;
  • Roh, Young-Bok (Department of Biology, College of Natural Science, Chosun University)
  • 정민주 (조선대학교 자연과학대학 생물학과) ;
  • 노영복 (조선대학교 자연과학대학 생물학과)
  • Published : 2006.12.31
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Abstract

A protective effect of activated charcoal against the acute lead poisoning of kidney was studied in mile. Mice approximately 30 gm in weight were grouped into the control, lead acetate-treated, and activated charcoal-treated after lead acetate groups. Lead acetate (60 mg/kg) and activated charcoal (40mg/kg) were delivered orally. Serum AST, ALT and glucose were measured and the ultrastructural alteration of liver was examined by electron microscopy. Activated charcoal decreased the increase of serum AST, ALT and glucose levels induced by lead. Lead acetate-treated hepatic cells characterized by irregular nuclei, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of riboscomes. Cells treated with activated charcoal were similar to those of the control group. In conclusion, activated charcoal may protect the lead-induced toxicity on liver.

본 연구는 급성 납 독성에 대한 활성탄의 방어효과를 알아보기 위하여 시도되었다. 30g내외의 생쥐를 대상으로 대조군, 납(60mg/kg) 투여군, 납 투여 후 활성탄(40mg/kg) 투여군으로 구분하여 구강투여 한 후 간장, 신장손상 억제 효과를 알아보기 위하여 생화학적 및 조직학적 실험을 실시하였다. 혈액중 aspartate amiotransferase(AST). alanine amino-transferase(ALT)그리고 glucose(Glu)의 수치는 활성탄 투여군이 납 투여군보다 감소되었다. 전자현미경적 관찰 결과, 간장에서 납 투여군은 간세포의 핵이 함입되어 불규칙했으며 미토콘드리아와 조면소포체의 수조가 팽대되고, 리보솜의 탈락이 관찰되었다. 활성탄 투여군은 핵이 대조군과 같은 형태가 관찰되었고, 전자밀도가 높은 미토콘드리아가 분포되어 있었으며, 리보솜이 부착된 상태로 층판구조를 형성하는 조면소포체가 관찰되었다. 이상과 같은 결과, 활성탄이 납 중독된 생쥐에서 납을 흡착시킴으로서 손상된 간장에 보호 효과가 있는 것으로 사료된다.

Keywords

References

  1. Alvares AP, Leigh S, Cohn J, Kappas A: Lead and methyl mercury: Effects of acute exposure on cytochrome P-450 and the mixed function oxidase system in the liver. J Exp Med 135 : 1406-1409, 1972
  2. Arimori K, Nakano M: Dose-dependency in the exsorption of theopylline and the intestinal dialysis of theophylline by oral ativated charcoal in rats. J Pharm Pharmacol 40(2) : 101-105, 1988 https://doi.org/10.1111/j.2042-7158.1988.tb05190.x
  3. Arimori K, Nakano M: Study on transport of disopyramide into the intestinal lumen aimed at gastrointestinal dialysis by activated charcoal in rats. J Pharm Pharmacol 41(7) : 445-449, 1989 https://doi.org/10.1111/j.2042-7158.1989.tb06498.x
  4. Batra N, Nehru B, Bansal MP: The effect of zinc supplementation on the effects of lead on the rat testis. Rspro Toxicol 12(5) : 535-540, 1998 https://doi.org/10.1016/S0890-6238(98)00030-6
  5. Chan O, Inouye K, Vranic M, Natthews SG: Hyperactivation of the hypothalamo-pituitary-adrenocortical axis in streptozotocindiabetes is associated with reduced stress responsiveness and decreased pituitary and adrenal sensitivity. Endocrinology 143(5) : 1761-1768, 2002 https://doi.org/10.1210/en.143.5.1761
  6. Chaurasia SS, Kar A: Protective effects of vitamin E against lead-induced deterioration of membrane associated type-I iodothyronine $5^{\prime}$-monodeiodinase $(5^{\prime}D-I)$ activity in male mice. Toxicol 124 : 203-209, 1997 https://doi.org/10.1016/S0300-483X(97)00155-8
  7. Chaurasia SS, Panda S, Kar A: Withania somnifera root extract in tje regulation of lead-induced oxidative damage in male mouse. Pharma Res 41(6) : 663-666, 2000 https://doi.org/10.1006/phrs.1999.0634
  8. Chung DL: ffects of garlic juice on the toxicity of lead in rat. Chosun University, 1991. (Korea)
  9. Corpas I, Antonio MT: Study of alterations produced by cadmium and cadmium/lead administration during gestational and early lactation periods in the reproductive organs of the rat. Ecotoxicol Environ Safety 41 : 180-188, 1998 https://doi.org/10.1006/eesa.1998.1690
  10. Corpas I, Beniro MJ, Marquina D, Castillo M, Lopez N, Antonio MT: Gestational and lactational lead intoxication produces alterations in the hepatic system of rat pups. Ecotoxi Enviro Sefety 51 : 35-43, 2002 https://doi.org/10.1006/eesa.2001.2102
  11. Curd-Sneed CD, Bordelon JG, Parks KS, Stewart JJ: Effects of activated charcoal and sorbitol on sodium pentobarbital absorption in the rat. J Toxicol Clin Toxicol 25(7) : 555-566, 1987 https://doi.org/10.3109/15563658708992657
  12. Daggett DA, Oberley TD, Nelson SA, Wright LS, Kornguth SE, Siegel FL: Effect of lead on rat kidney and liver: GST expression and oxidative stress. Toxicol 128 : 191-206, 1998 https://doi.org/10.1016/S0300-483X(98)00080-8
  13. Davis DR, Yeary RA, Lee K: Activated charcoal decreases plama bilirubin levels in the hyperbilirubinemic rat. Pediatr Res 17(3) : 208-209, 1983 https://doi.org/10.1203/00006450-198303000-00008
  14. Ernesto OH, Nicholas RD, Klaus H, Lawrence B, Joan C: Ultrastructural changes in the liver of Baboons following lead and endotoxin administration. Int Aca Path 30 : 311-319, 1974
  15. Ernesto OH, Rafael AT, Luzio JL: Ultrastructural alterations of liver and spleen following acute lead administruction in rat. Esp Mol Path 17 : 159-170, 1972 https://doi.org/10.1016/0014-4800(72)90065-2
  16. Flora SJS, Pande M, Mehta A: Beneficial effect of combined administration of some naturally occurring antioxidants (vitamins) and thiol chelators in the treatment of chronic lead intoxication. Chemico-Biololgical Interaction 145 : 267-280, 2003 https://doi.org/10.1016/S0009-2797(03)00025-5
  17. Fonia O: Down-regulation of hepatic peripheral-type benzodiazepine receptors caused by acute lead intoxication. Eur J Pharmacol Environ Toxicol Pharmarcol Section 293 : 335-339, 1995 https://doi.org/10.1016/0926-6917(95)90053-5
  18. Han SH, Shin MK, Kim YW, Lim SJ: Effects of Methanol Extracts of Black Soybean on Enzymes Activities of Serum in Rats Fed Pb and Cd Solution. J Korea Soc Food Nutr 29(2) : 193-197, 2000. (Korea)
  19. Han SH: Effects of Extracts of Pueraria radix on Enzymes Activities of Serum and Lead Level of the Tissues of the Pb-administered Rats. Korean J Food Sci Technol 32(4) : 914-919, 2000. (Korea)
  20. Im JG, Rhee SJ: Effect of Dietary Selenium Levels on Antioxidative Defense System and Oxidatvie Damage of Liver Tissue in Lead Administered Rats. J East Asian Soc Dietary Life 11(4) : 259-267, 2001. (Korea)
  21. Johnson JB, Sparberg M: Try activated charcoal for ostomates. Patient Care 21 : 152, 1977
  22. Jung EH, Jo SJ: Moderation of the bitter taste of extracts from Pueraria Radix by charcoal powder. Korea J Liv Sci Assoc 9(2) : 215-221, 2000. (Korea)
  23. Karmakar N, Saxena R, Anand S: Histopathological changes induced in rat tissues by oral intake of lead acetate. Enviro Res 41 : 23-28, 1986 https://doi.org/10.1016/S0013-9351(86)80164-5
  24. Ku Y, Fukumoto T, Tominaga M, Iwasaki T, Maeda I: Single catheter technique of hepatic venous isolation and extracorporeal charcoal hemoperfusion for malignant liver tumors. Am J Surg 173(2) : 103-109, 1997 https://doi.org/10.1016/S0002-9610(96)00422-9
  25. Lee SI: Effect of extract of Lycii Fructus on Hematological properties and Pb levels of tissues of the Pb-administered rats. DongA University, 1997. (Korea)
  26. Leuchtenberger C, Leuchtenberger R: Cytologic and cytochemical effects on primary mouse kidney tissue and lung organ cultures after exposure to whole' fresh smoke and its gas phase from unfiltered' charcoal-filtered' and ciger tobacco cigarettes. Cancer Res 29(4) : 862-872, 1969
  27. Mahaffey KR, Capar SG, Gladen BC, Fowler BA: Concurrent exposure to lead, cadmium, and arsenic. Effects on toxicity and tissue metal concentrations in the rat. J Lab Clin Med 98(4) : 463-481, 1981
  28. Merino GE, Jetzer T, Doizki WM, Najarian JS: Methionineinduced hepatic coma in dogs. Am J Surg 130(1) : 41-46, 1975 https://doi.org/10.1016/0002-9610(75)90454-7
  29. Milosevic N, Maier P: Lead stimulates intercellular singalling between hepatocytes and kuffer cells. Euro J Phama 401 : 317-328, 2000 https://doi.org/10.1016/S0014-2999(00)00473-8
  30. Moon SS, Shin CW, Kang GH, Joo ST, Park GB: Effects of Dietary Activated Carbon on Physico-Chemical Characteristics and Fatty Acid Composition of Pork. Korean J Food Sci Ani Resources 22(2) : 145-150, 2002. (Korea)
  31. Oberley TD, Friedman AL, Moser R, Siegel FL: Effect of lead administration on developing rat kidney. Toxicol Appl Pharmacol 131 : 94-107, 1995 https://doi.org/10.1006/taap.1995.1051
  32. Orisakwe OE, Ilondu NA, Afonne JO, Ofoefule S, Orish CN: Acceleration of body clearance of diethylcarbamazine by oral activated charcoal. Pharmacol Res 42(2) : 167-170, 2000 https://doi.org/10.1006/phrs.2000.0667
  33. Pande M, Flora SJS: Lead induced oxidative damage and its response to combined administration of $\alpha$-lipoic acid and succimers in rats. Toxicology 177 : 187-196, 2002 https://doi.org/10.1016/S0300-483X(02)00223-8
  34. Park CI, Kim YJ: Effect of Additions of Supplemental Activated Carbon on the Fatty Acid, Meat Color and Minerals of Chicken Meat. Korean J Food Sci Ani Resources 21(4) : 285-291, 2001. (Korea)
  35. Patra RC, Swarup D, Dwivedi SK: Antioxidant effects of $\alpha$ tocopherol, ascorbic acid and L-methionine on lead induced oxidative stress to the liver, kidney and branin in rats. Toxicology 162 : 81-88, 2001 https://doi.org/10.1016/S0300-483X(01)00345-6
  36. Sastry KV, Subhadra K: In vivo effects of cadmium on some enzyme activities in tissues of the freshwater catfish, Heteropneustes fossilis. Environ Res 36(1) : 32-45, 1985 https://doi.org/10.1016/0013-9351(85)90005-2
  37. Schroeder HA, Brettleboro V, Tipton IH: The human body burden of lead. Arch Environ Health 17 : 986-978, 1968 https://doi.org/10.1080/00039896.1968.10665357
  38. Senapati SK, Dey S, Dwivedi SK, Swarup D: Effect of gralic (Allium sativum L.) extract on tissue lead level in rats. J Ethnopharm 76 : 229-232, 2001 https://doi.org/10.1016/S0378-8741(01)00237-9
  39. Sheehan JE, Pitot HC, Kasper CB: Transcriptional regulation and localization of the tissue-specific induction of epoxide hydrolase by lead acetate in rat kidney. J Biol Chem 266(8) : 5122-5127, 1991
  40. Sheo HJ, Kim YS, Kin KS, Jung DL: Effect of gralic on toxicity of mercury in rat. J Korean Soc Food Nutr 23 : 908-915, 1994. (Korea)
  41. Sheo HJ, Lim HJ, Jung DL: Effects of Onion Juice on Toxicity of Lead in Rat. J Korea Soc Food Nutr 22(2) : 138-143, 1993. (Korea)
  42. Traeger SM, Haug MT: Reduction of diazepam serum half life and revesal of coma by activated charcoal in a patient with severe liver cisease. J Toxicol Clin Toxicol 24(4) : 329-337, 1986 https://doi.org/10.3109/15563658608992597
  43. Yum KS, Park KH, Yang NK, Ahn LT, Ko JS: Ultrastructural influence of heavy metallic agents on the proximal convoluted tubule of mice. J Soonchunhyang Med 1(2) : 719- 733, 1995. (Korea)